Genome Mining of Non-Conventional Yeasts: Search and Analysis of MAL Clusters and Proteins
Genomic clustering of functionally related genes is rare in yeasts and other eukaryotes with only few examples available. Here, we summarize our data on a nontelomeric MAL cluster of a non-conventional methylotrophic yeast Ogataea (Hansenula) polymorpha containing genes for α-glucosidase M...
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doaj-7f55dcbdaf0043e69de4bb396aad926d2020-11-24T23:25:20ZengMDPI AGGenes2073-44252018-07-019735410.3390/genes9070354genes9070354Genome Mining of Non-Conventional Yeasts: Search and Analysis of MAL Clusters and ProteinsKatrin Viigand0Kristina Põšnograjeva1Triinu Visnapuu2Tiina Alamäe3Department of Genetics, Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, EstoniaDepartment of Genetics, Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, EstoniaDepartment of Genetics, Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, EstoniaDepartment of Genetics, Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010 Tartu, EstoniaGenomic clustering of functionally related genes is rare in yeasts and other eukaryotes with only few examples available. Here, we summarize our data on a nontelomeric MAL cluster of a non-conventional methylotrophic yeast Ogataea (Hansenula) polymorpha containing genes for α-glucosidase MAL1, α-glucoside permease MAL2 and two hypothetical transcriptional activators. Using genome mining, we detected MAL clusters of varied number, position and composition in many other maltose-assimilating non-conventional yeasts from different phylogenetic groups. The highest number of MAL clusters was detected in Lipomyces starkeyi while no MAL clusters were found in Schizosaccharomyces pombe and Blastobotrys adeninivorans. Phylograms of α-glucosidases and α-glucoside transporters of yeasts agreed with phylogenesis of the respective yeast species. Substrate specificity of unstudied α-glucosidases was predicted from protein sequence analysis. Specific activities of Scheffersomycesstipitis α-glucosidases MAL7, MAL8, and MAL9 heterologously expressed in Escherichia coli confirmed the correctness of the prediction—these proteins were verified promiscuous maltase-isomaltases. α-Glucosidases of earlier diverged yeasts L. starkeyi, B. adeninivorans and S. pombe showed sequence relatedness with α-glucosidases of filamentous fungi and bacilli.http://www.mdpi.com/2073-4425/9/7/354α-glucosidasemaltaseisomaltaseα-glucoside permeasegene clusterprotein evolutionmethylotrophic yeastMAL-locus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katrin Viigand Kristina Põšnograjeva Triinu Visnapuu Tiina Alamäe |
spellingShingle |
Katrin Viigand Kristina Põšnograjeva Triinu Visnapuu Tiina Alamäe Genome Mining of Non-Conventional Yeasts: Search and Analysis of MAL Clusters and Proteins Genes α-glucosidase maltase isomaltase α-glucoside permease gene cluster protein evolution methylotrophic yeast MAL-locus |
author_facet |
Katrin Viigand Kristina Põšnograjeva Triinu Visnapuu Tiina Alamäe |
author_sort |
Katrin Viigand |
title |
Genome Mining of Non-Conventional Yeasts: Search and Analysis of MAL Clusters and Proteins |
title_short |
Genome Mining of Non-Conventional Yeasts: Search and Analysis of MAL Clusters and Proteins |
title_full |
Genome Mining of Non-Conventional Yeasts: Search and Analysis of MAL Clusters and Proteins |
title_fullStr |
Genome Mining of Non-Conventional Yeasts: Search and Analysis of MAL Clusters and Proteins |
title_full_unstemmed |
Genome Mining of Non-Conventional Yeasts: Search and Analysis of MAL Clusters and Proteins |
title_sort |
genome mining of non-conventional yeasts: search and analysis of mal clusters and proteins |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2018-07-01 |
description |
Genomic clustering of functionally related genes is rare in yeasts and other eukaryotes with only few examples available. Here, we summarize our data on a nontelomeric MAL cluster of a non-conventional methylotrophic yeast Ogataea (Hansenula) polymorpha containing genes for α-glucosidase MAL1, α-glucoside permease MAL2 and two hypothetical transcriptional activators. Using genome mining, we detected MAL clusters of varied number, position and composition in many other maltose-assimilating non-conventional yeasts from different phylogenetic groups. The highest number of MAL clusters was detected in Lipomyces starkeyi while no MAL clusters were found in Schizosaccharomyces pombe and Blastobotrys adeninivorans. Phylograms of α-glucosidases and α-glucoside transporters of yeasts agreed with phylogenesis of the respective yeast species. Substrate specificity of unstudied α-glucosidases was predicted from protein sequence analysis. Specific activities of Scheffersomycesstipitis α-glucosidases MAL7, MAL8, and MAL9 heterologously expressed in Escherichia coli confirmed the correctness of the prediction—these proteins were verified promiscuous maltase-isomaltases. α-Glucosidases of earlier diverged yeasts L. starkeyi, B. adeninivorans and S. pombe showed sequence relatedness with α-glucosidases of filamentous fungi and bacilli. |
topic |
α-glucosidase maltase isomaltase α-glucoside permease gene cluster protein evolution methylotrophic yeast MAL-locus |
url |
http://www.mdpi.com/2073-4425/9/7/354 |
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