A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO<sub>2</sub> Revealed Different Responses in the Polyploid <i>Coffea Arabica</i> and Its Diploid Progenitor <i>C. Canephora</i>
Understanding the effect of extreme temperatures and elevated air (CO<sub>2</sub>) is crucial for mitigating the impacts of the coffee industry. In this work, leaf transcriptomic changes were evaluated in the diploid <i>C. canephora</i> and its polyploid <i>C. arabica&l...
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doaj-7f5b3e56b010415794bc90c0129a1a9b2021-03-19T00:06:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01223125312510.3390/ijms22063125A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO<sub>2</sub> Revealed Different Responses in the Polyploid <i>Coffea Arabica</i> and Its Diploid Progenitor <i>C. Canephora</i>Isabel Marques0Isabel Fernandes1Octávio S. Paulo2Fernando C. Lidon3Fábio M. DaMatta4José C. Ramalho5Ana I. Ribeiro-Barros6Plant-Environment Interactions and Biodiversity Lab (PlantStress&Biodiversity), Forest Research Centre (CEF), Instituto Superior de Agronomia (ISA), Universidade de Lisboa, 2784-505 Oeiras and Tapada da Ajuda, 1349-017 Lisboa, PortugalComputational Biology and Population Genomics Group, Centre for Ecology, Evolution and Environmental Changes (cE3c), Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, PortugalComputational Biology and Population Genomics Group, Centre for Ecology, Evolution and Environmental Changes (cE3c), Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, PortugalGeoBioSciences, GeoTechnologies and GeoEngineering (GeoBioTec), Faculdade de Ciências e Tecnologia (FCT), Universidade NOVA de Lisboa (UNL), 2829-516 Monte de Caparica, PortugalDepartamento de Biologia Vegetal, Universidade Federal Viçosa (UFV), Viçosa 36570-090, MG, BrazilPlant-Environment Interactions and Biodiversity Lab (PlantStress&Biodiversity), Forest Research Centre (CEF), Instituto Superior de Agronomia (ISA), Universidade de Lisboa, 2784-505 Oeiras and Tapada da Ajuda, 1349-017 Lisboa, PortugalPlant-Environment Interactions and Biodiversity Lab (PlantStress&Biodiversity), Forest Research Centre (CEF), Instituto Superior de Agronomia (ISA), Universidade de Lisboa, 2784-505 Oeiras and Tapada da Ajuda, 1349-017 Lisboa, PortugalUnderstanding the effect of extreme temperatures and elevated air (CO<sub>2</sub>) is crucial for mitigating the impacts of the coffee industry. In this work, leaf transcriptomic changes were evaluated in the diploid <i>C. canephora</i> and its polyploid <i>C. arabica</i>, grown at 25 °C and at two supra-optimal temperatures (37 °C, 42 °C), under ambient (aCO<sub>2</sub>) or elevated air CO<sub>2</sub> (eCO<sub>2</sub>). Both species expressed fewer genes as temperature rose, although a high number of differentially expressed genes (DEGs) were observed, especially at 42 °C. An enrichment analysis revealed that the two species reacted differently to the high temperatures but with an overall up-regulation of the photosynthetic machinery until 37 °C. Although eCO<sub>2</sub> helped to release stress, 42 °C had a severe impact on both species. A total of 667 photosynthetic and biochemical related-DEGs were altered with high temperatures and eCO<sub>2</sub>, which may be used as key probe genes in future studies. This was mostly felt in <i>C. arabica</i>, where genes related to ribulose-bisphosphate carboxylase (RuBisCO) activity, chlorophyll a-b binding, and the reaction centres of photosystems I and II were down-regulated, especially under 42°C, regardless of CO<sub>2</sub>. Transcriptomic changes showed that both species were strongly affected by the highest temperature, although they can endure higher temperatures (37 °C) than previously assumed.https://www.mdpi.com/1422-0067/22/6/3125climate changescoffeeelevated air (CO<sub>2</sub>)functional analysishigh temperaturesleaf RNAseq |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Isabel Marques Isabel Fernandes Octávio S. Paulo Fernando C. Lidon Fábio M. DaMatta José C. Ramalho Ana I. Ribeiro-Barros |
spellingShingle |
Isabel Marques Isabel Fernandes Octávio S. Paulo Fernando C. Lidon Fábio M. DaMatta José C. Ramalho Ana I. Ribeiro-Barros A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO<sub>2</sub> Revealed Different Responses in the Polyploid <i>Coffea Arabica</i> and Its Diploid Progenitor <i>C. Canephora</i> International Journal of Molecular Sciences climate changes coffee elevated air (CO<sub>2</sub>) functional analysis high temperatures leaf RNAseq |
author_facet |
Isabel Marques Isabel Fernandes Octávio S. Paulo Fernando C. Lidon Fábio M. DaMatta José C. Ramalho Ana I. Ribeiro-Barros |
author_sort |
Isabel Marques |
title |
A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO<sub>2</sub> Revealed Different Responses in the Polyploid <i>Coffea Arabica</i> and Its Diploid Progenitor <i>C. Canephora</i> |
title_short |
A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO<sub>2</sub> Revealed Different Responses in the Polyploid <i>Coffea Arabica</i> and Its Diploid Progenitor <i>C. Canephora</i> |
title_full |
A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO<sub>2</sub> Revealed Different Responses in the Polyploid <i>Coffea Arabica</i> and Its Diploid Progenitor <i>C. Canephora</i> |
title_fullStr |
A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO<sub>2</sub> Revealed Different Responses in the Polyploid <i>Coffea Arabica</i> and Its Diploid Progenitor <i>C. Canephora</i> |
title_full_unstemmed |
A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO<sub>2</sub> Revealed Different Responses in the Polyploid <i>Coffea Arabica</i> and Its Diploid Progenitor <i>C. Canephora</i> |
title_sort |
transcriptomic approach to understanding the combined impacts of supra-optimal temperatures and co<sub>2</sub> revealed different responses in the polyploid <i>coffea arabica</i> and its diploid progenitor <i>c. canephora</i> |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-03-01 |
description |
Understanding the effect of extreme temperatures and elevated air (CO<sub>2</sub>) is crucial for mitigating the impacts of the coffee industry. In this work, leaf transcriptomic changes were evaluated in the diploid <i>C. canephora</i> and its polyploid <i>C. arabica</i>, grown at 25 °C and at two supra-optimal temperatures (37 °C, 42 °C), under ambient (aCO<sub>2</sub>) or elevated air CO<sub>2</sub> (eCO<sub>2</sub>). Both species expressed fewer genes as temperature rose, although a high number of differentially expressed genes (DEGs) were observed, especially at 42 °C. An enrichment analysis revealed that the two species reacted differently to the high temperatures but with an overall up-regulation of the photosynthetic machinery until 37 °C. Although eCO<sub>2</sub> helped to release stress, 42 °C had a severe impact on both species. A total of 667 photosynthetic and biochemical related-DEGs were altered with high temperatures and eCO<sub>2</sub>, which may be used as key probe genes in future studies. This was mostly felt in <i>C. arabica</i>, where genes related to ribulose-bisphosphate carboxylase (RuBisCO) activity, chlorophyll a-b binding, and the reaction centres of photosystems I and II were down-regulated, especially under 42°C, regardless of CO<sub>2</sub>. Transcriptomic changes showed that both species were strongly affected by the highest temperature, although they can endure higher temperatures (37 °C) than previously assumed. |
topic |
climate changes coffee elevated air (CO<sub>2</sub>) functional analysis high temperatures leaf RNAseq |
url |
https://www.mdpi.com/1422-0067/22/6/3125 |
work_keys_str_mv |
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