Expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm
Abstract Evaluation of transcriptome data in combination with QTL information has been applied in many crops to study the expression of genes responsible for specific phenotypes. In oil palm, the mesocarp oil extracted from E. oleifera × E. guineensis interspecific hybrids is known to have lower pal...
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doaj-7f82132b81d7401081c8d63c587ed9d52021-10-03T11:24:37ZengNature Publishing GroupScientific Reports2045-23222020-10-0110111510.1038/s41598-020-73170-5Expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palmNgoot-Chin Ting0Katrina Sherbina1Jia-Shiun Khoo2Katialisa Kamaruddin3Pek-Lan Chan4Kuang-Lim Chan5Mohd Amin Ab Halim6Kandha Sritharan7Zulkifli Yaakub8Sean Mayes9Festo Massawe10Peter L. Chang11Sergey V. Nuzhdin12Ravigadevi Sambanthamurthi13Rajinder Singh14Advanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board (MPOB)Quantitative and Computational Biology Section, University of Southern CaliforniaCodon Genomics Sdn. Bhd. MalaysiaAdvanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board (MPOB)Advanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board (MPOB)Advanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board (MPOB)Advanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board (MPOB)United Plantations BhdAdvanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board (MPOB)Plant and Crop Sciences, Sutton Bonington Campus, Sutton Bonington, University of NottinghamBiotechnology Research Centre, School of Biosciences, University of Nottingham Malaysia CampusMolecular and Computational Biology Section, University of Southern CaliforniaMolecular and Computational Biology Section, University of Southern CaliforniaAdvanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board (MPOB)Advanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board (MPOB)Abstract Evaluation of transcriptome data in combination with QTL information has been applied in many crops to study the expression of genes responsible for specific phenotypes. In oil palm, the mesocarp oil extracted from E. oleifera × E. guineensis interspecific hybrids is known to have lower palmitic acid (C16:0) content compared to pure African palms. The present study demonstrates the effectiveness of transcriptome data in revealing the expression profiles of genes in the fatty acid (FA) and triacylglycerol (TAG) biosynthesis processes in interspecific hybrids. The transcriptome assembly yielded 43,920 putative genes of which a large proportion were homologous to known genes in the public databases. Most of the genes encoding key enzymes involved in the FA and TAG synthesis pathways were identified. Of these, 27, including two candidate genes located within the QTL associated with C16:0 content, showed differential expression between developmental stages, populations and/or palms with contrasting C16:0 content. Further evaluation using quantitative real-time PCR revealed that differentially expressed patterns are generally consistent with those observed in the transcriptome data. Our results also suggest that different isoforms are likely to be responsible for some of the variation observed in FA composition of interspecific hybrids.https://doi.org/10.1038/s41598-020-73170-5 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ngoot-Chin Ting Katrina Sherbina Jia-Shiun Khoo Katialisa Kamaruddin Pek-Lan Chan Kuang-Lim Chan Mohd Amin Ab Halim Kandha Sritharan Zulkifli Yaakub Sean Mayes Festo Massawe Peter L. Chang Sergey V. Nuzhdin Ravigadevi Sambanthamurthi Rajinder Singh |
spellingShingle |
Ngoot-Chin Ting Katrina Sherbina Jia-Shiun Khoo Katialisa Kamaruddin Pek-Lan Chan Kuang-Lim Chan Mohd Amin Ab Halim Kandha Sritharan Zulkifli Yaakub Sean Mayes Festo Massawe Peter L. Chang Sergey V. Nuzhdin Ravigadevi Sambanthamurthi Rajinder Singh Expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm Scientific Reports |
author_facet |
Ngoot-Chin Ting Katrina Sherbina Jia-Shiun Khoo Katialisa Kamaruddin Pek-Lan Chan Kuang-Lim Chan Mohd Amin Ab Halim Kandha Sritharan Zulkifli Yaakub Sean Mayes Festo Massawe Peter L. Chang Sergey V. Nuzhdin Ravigadevi Sambanthamurthi Rajinder Singh |
author_sort |
Ngoot-Chin Ting |
title |
Expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm |
title_short |
Expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm |
title_full |
Expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm |
title_fullStr |
Expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm |
title_full_unstemmed |
Expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm |
title_sort |
expression of fatty acid and triacylglycerol synthesis genes in interspecific hybrids of oil palm |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2020-10-01 |
description |
Abstract Evaluation of transcriptome data in combination with QTL information has been applied in many crops to study the expression of genes responsible for specific phenotypes. In oil palm, the mesocarp oil extracted from E. oleifera × E. guineensis interspecific hybrids is known to have lower palmitic acid (C16:0) content compared to pure African palms. The present study demonstrates the effectiveness of transcriptome data in revealing the expression profiles of genes in the fatty acid (FA) and triacylglycerol (TAG) biosynthesis processes in interspecific hybrids. The transcriptome assembly yielded 43,920 putative genes of which a large proportion were homologous to known genes in the public databases. Most of the genes encoding key enzymes involved in the FA and TAG synthesis pathways were identified. Of these, 27, including two candidate genes located within the QTL associated with C16:0 content, showed differential expression between developmental stages, populations and/or palms with contrasting C16:0 content. Further evaluation using quantitative real-time PCR revealed that differentially expressed patterns are generally consistent with those observed in the transcriptome data. Our results also suggest that different isoforms are likely to be responsible for some of the variation observed in FA composition of interspecific hybrids. |
url |
https://doi.org/10.1038/s41598-020-73170-5 |
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