Map-Based Cloning, Phylogenetic, and Microsynteny Analyses of <i>ZmMs20</i> Gene Regulating Male Fertility in Maize
Genic male sterility (GMS) mutant is a useful germplasm resource for both theory research and production practice. The identification and characterization of GMS genes, and assessment of male-sterility stability of GMS mutant under different genetic backgrounds in <i>Zea may</i> (maize)...
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doaj-7f85be8beef046d1b289c257126ed4ad2020-11-24T21:21:35ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-03-01206141110.3390/ijms20061411ijms20061411Map-Based Cloning, Phylogenetic, and Microsynteny Analyses of <i>ZmMs20</i> Gene Regulating Male Fertility in MaizeYanbo Wang0Dongcheng Liu1Youhui Tian2Suowei Wu3Xueli An4Zhenying Dong5Simiao Zhang6Jianxi Bao7Ziwen Li8Jinping Li9Xiangyuan Wan10Biology and Agriculture Research Center, University of Science and Technology Beijing, Beijing 100024, ChinaBiology and Agriculture Research Center, University of Science and Technology Beijing, Beijing 100024, ChinaBiology and Agriculture Research Center, University of Science and Technology Beijing, Beijing 100024, ChinaBiology and Agriculture Research Center, University of Science and Technology Beijing, Beijing 100024, ChinaBiology and Agriculture Research Center, University of Science and Technology Beijing, Beijing 100024, ChinaBiology and Agriculture Research Center, University of Science and Technology Beijing, Beijing 100024, ChinaBiology and Agriculture Research Center, University of Science and Technology Beijing, Beijing 100024, ChinaBiology and Agriculture Research Center, University of Science and Technology Beijing, Beijing 100024, ChinaBiology and Agriculture Research Center, University of Science and Technology Beijing, Beijing 100024, ChinaBeijing Engineering Laboratory of Main Crop Bio-Tech Breeding, Beijing International Science and Technology Cooperation Base of Bio-Tech Breeding, Beijing Solidwill Sci-Tech Co. Ltd., Beijing 100192, ChinaBiology and Agriculture Research Center, University of Science and Technology Beijing, Beijing 100024, ChinaGenic male sterility (GMS) mutant is a useful germplasm resource for both theory research and production practice. The identification and characterization of GMS genes, and assessment of male-sterility stability of GMS mutant under different genetic backgrounds in <i>Zea may</i> (maize) have (1) deepened our understanding of the molecular mechanisms controlling anther and pollen development, and (2) enabled the development and efficient use of many biotechnology-based male-sterility (BMS) systems for hybrid breeding. Here, we reported a complete GMS mutant (<i>ms20</i>), which displays abnormal anther cuticle and pollen development. Its fertility restorer gene <i>ZmMs20</i> was found to be a new allele of <i>IPE1</i> encoding a glucose methanol choline (GMC) oxidoreductase involved in lipid metabolism in anther. Phylogenetic and microsynteny analyses showed that ZmMs20 was conserved among gramineous species, which provide clues for creating GMS materials in other crops. Additionally, among the 17 maize cloned GMS genes, <i>ZmMs20</i> was found to be similar to the expression patterns of <i>Ms7</i>, <i>Ms26</i>, <i>Ms6021</i>, <i>APV1</i>, and <i>IG1</i> genes, which will give some clues for deciphering their functional relationships in regulating male fertility. Finally, two functional markers of <i>ZmMs20/ms20</i> were developed and tested for creating maize <i>ms20</i> male-sterility lines in 353 genetic backgrounds, and then an artificial maintainer line of <i>ms20</i> GMS mutation was created by using <i>ZmMs20</i> gene, <i>ms20</i> mutant, and BMS system. This work will promote our understanding of functional mechanisms of male fertility and facilitate molecular breeding of <i>ms20</i> male-sterility lines for hybrid seed production in maize.https://www.mdpi.com/1422-0067/20/6/1411genic male sterility (GMS)anther developmentglucose methanol choline (GMC) oxidoreductasehybrid breeding<i>Zea may</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yanbo Wang Dongcheng Liu Youhui Tian Suowei Wu Xueli An Zhenying Dong Simiao Zhang Jianxi Bao Ziwen Li Jinping Li Xiangyuan Wan |
spellingShingle |
Yanbo Wang Dongcheng Liu Youhui Tian Suowei Wu Xueli An Zhenying Dong Simiao Zhang Jianxi Bao Ziwen Li Jinping Li Xiangyuan Wan Map-Based Cloning, Phylogenetic, and Microsynteny Analyses of <i>ZmMs20</i> Gene Regulating Male Fertility in Maize International Journal of Molecular Sciences genic male sterility (GMS) anther development glucose methanol choline (GMC) oxidoreductase hybrid breeding <i>Zea may</i> |
author_facet |
Yanbo Wang Dongcheng Liu Youhui Tian Suowei Wu Xueli An Zhenying Dong Simiao Zhang Jianxi Bao Ziwen Li Jinping Li Xiangyuan Wan |
author_sort |
Yanbo Wang |
title |
Map-Based Cloning, Phylogenetic, and Microsynteny Analyses of <i>ZmMs20</i> Gene Regulating Male Fertility in Maize |
title_short |
Map-Based Cloning, Phylogenetic, and Microsynteny Analyses of <i>ZmMs20</i> Gene Regulating Male Fertility in Maize |
title_full |
Map-Based Cloning, Phylogenetic, and Microsynteny Analyses of <i>ZmMs20</i> Gene Regulating Male Fertility in Maize |
title_fullStr |
Map-Based Cloning, Phylogenetic, and Microsynteny Analyses of <i>ZmMs20</i> Gene Regulating Male Fertility in Maize |
title_full_unstemmed |
Map-Based Cloning, Phylogenetic, and Microsynteny Analyses of <i>ZmMs20</i> Gene Regulating Male Fertility in Maize |
title_sort |
map-based cloning, phylogenetic, and microsynteny analyses of <i>zmms20</i> gene regulating male fertility in maize |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-03-01 |
description |
Genic male sterility (GMS) mutant is a useful germplasm resource for both theory research and production practice. The identification and characterization of GMS genes, and assessment of male-sterility stability of GMS mutant under different genetic backgrounds in <i>Zea may</i> (maize) have (1) deepened our understanding of the molecular mechanisms controlling anther and pollen development, and (2) enabled the development and efficient use of many biotechnology-based male-sterility (BMS) systems for hybrid breeding. Here, we reported a complete GMS mutant (<i>ms20</i>), which displays abnormal anther cuticle and pollen development. Its fertility restorer gene <i>ZmMs20</i> was found to be a new allele of <i>IPE1</i> encoding a glucose methanol choline (GMC) oxidoreductase involved in lipid metabolism in anther. Phylogenetic and microsynteny analyses showed that ZmMs20 was conserved among gramineous species, which provide clues for creating GMS materials in other crops. Additionally, among the 17 maize cloned GMS genes, <i>ZmMs20</i> was found to be similar to the expression patterns of <i>Ms7</i>, <i>Ms26</i>, <i>Ms6021</i>, <i>APV1</i>, and <i>IG1</i> genes, which will give some clues for deciphering their functional relationships in regulating male fertility. Finally, two functional markers of <i>ZmMs20/ms20</i> were developed and tested for creating maize <i>ms20</i> male-sterility lines in 353 genetic backgrounds, and then an artificial maintainer line of <i>ms20</i> GMS mutation was created by using <i>ZmMs20</i> gene, <i>ms20</i> mutant, and BMS system. This work will promote our understanding of functional mechanisms of male fertility and facilitate molecular breeding of <i>ms20</i> male-sterility lines for hybrid seed production in maize. |
topic |
genic male sterility (GMS) anther development glucose methanol choline (GMC) oxidoreductase hybrid breeding <i>Zea may</i> |
url |
https://www.mdpi.com/1422-0067/20/6/1411 |
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