Optimization of chromatographic conditions and comparison of extraction efficiencies of four different methods for determination and quantification of pesticide content in bovine milk by UFLC-MS/MS
This paper describes the optimization of a multiresidue chromatographic analysis for the identification and quantification of 20 pesticides in bovine milk, including three carbamates, a carbamate oxime, six organophosphates, two strobilurins, a pyrethroid, an oxazolidinedione, an aryloxyphenoxypropi...
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Sociedade Brasileira de Química
2014-01-01
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doaj-7fba0823f2294075ad11fdb78486e9402020-11-24T21:20:02ZengSociedade Brasileira de QuímicaQuímica Nova1678-70642014-01-0137101699170610.5935/0100-4042.20140264S0100-40422014001000020Optimization of chromatographic conditions and comparison of extraction efficiencies of four different methods for determination and quantification of pesticide content in bovine milk by UFLC-MS/MSFabiano A. S. Oliveira0Fernando D. Madureira1Renata P. Lopes2Marina G. Ferreira3Benito Soto-Blanco4Marília M. Melo5Ministério da Agricultura, Pecuária e AbastecimentoMinistério da Agricultura, Pecuária e AbastecimentoMinistério da Agricultura, Pecuária e AbastecimentoUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisThis paper describes the optimization of a multiresidue chromatographic analysis for the identification and quantification of 20 pesticides in bovine milk, including three carbamates, a carbamate oxime, six organophosphates, two strobilurins, a pyrethroid, an oxazolidinedione, an aryloxyphenoxypropionate acid/ester, a neonicotinoid, a dicarboximide, and three triazoles. The influences of different chromatographic columns and gradients were evaluated. Furthermore, four different extraction methods were evaluated; each utilized both different solvents, including ethyl acetate, methanol, and acetonitrile, and different workup steps. The best results were obtained by a modified QuEChERS method that lacked a workup step, and that included freezing the sample for 2 hours at -20 ºC. The results were satisfactory, yielding coefficients of variation of less than 20%, with the exception of the 50 µg L-1 sample of famoxadone, and recoveries between 70 and 120%, with the exception of acephate and bifenthrin; however, both analytes exhibited coefficients of variation of less than 20%.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014001000020&lng=en&tlng=enpesticidesmilkmultiresidue analysismass spectrometer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fabiano A. S. Oliveira Fernando D. Madureira Renata P. Lopes Marina G. Ferreira Benito Soto-Blanco Marília M. Melo |
spellingShingle |
Fabiano A. S. Oliveira Fernando D. Madureira Renata P. Lopes Marina G. Ferreira Benito Soto-Blanco Marília M. Melo Optimization of chromatographic conditions and comparison of extraction efficiencies of four different methods for determination and quantification of pesticide content in bovine milk by UFLC-MS/MS Química Nova pesticides milk multiresidue analysis mass spectrometer |
author_facet |
Fabiano A. S. Oliveira Fernando D. Madureira Renata P. Lopes Marina G. Ferreira Benito Soto-Blanco Marília M. Melo |
author_sort |
Fabiano A. S. Oliveira |
title |
Optimization of chromatographic conditions and comparison of extraction efficiencies of four different methods for determination and quantification of pesticide content in bovine milk by UFLC-MS/MS |
title_short |
Optimization of chromatographic conditions and comparison of extraction efficiencies of four different methods for determination and quantification of pesticide content in bovine milk by UFLC-MS/MS |
title_full |
Optimization of chromatographic conditions and comparison of extraction efficiencies of four different methods for determination and quantification of pesticide content in bovine milk by UFLC-MS/MS |
title_fullStr |
Optimization of chromatographic conditions and comparison of extraction efficiencies of four different methods for determination and quantification of pesticide content in bovine milk by UFLC-MS/MS |
title_full_unstemmed |
Optimization of chromatographic conditions and comparison of extraction efficiencies of four different methods for determination and quantification of pesticide content in bovine milk by UFLC-MS/MS |
title_sort |
optimization of chromatographic conditions and comparison of extraction efficiencies of four different methods for determination and quantification of pesticide content in bovine milk by uflc-ms/ms |
publisher |
Sociedade Brasileira de Química |
series |
Química Nova |
issn |
1678-7064 |
publishDate |
2014-01-01 |
description |
This paper describes the optimization of a multiresidue chromatographic analysis for the identification and quantification of 20 pesticides in bovine milk, including three carbamates, a carbamate oxime, six organophosphates, two strobilurins, a pyrethroid, an oxazolidinedione, an aryloxyphenoxypropionate acid/ester, a neonicotinoid, a dicarboximide, and three triazoles. The influences of different chromatographic columns and gradients were evaluated. Furthermore, four different extraction methods were evaluated; each utilized both different solvents, including ethyl acetate, methanol, and acetonitrile, and different workup steps. The best results were obtained by a modified QuEChERS method that lacked a workup step, and that included freezing the sample for 2 hours at -20 ºC. The results were satisfactory, yielding coefficients of variation of less than 20%, with the exception of the 50 µg L-1 sample of famoxadone, and recoveries between 70 and 120%, with the exception of acephate and bifenthrin; however, both analytes exhibited coefficients of variation of less than 20%. |
topic |
pesticides milk multiresidue analysis mass spectrometer |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014001000020&lng=en&tlng=en |
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