Genotyping of <i>MSP3</i>β gene in Indian <i>Plasmodium vivax</i>
<b>Background & objectives:</b> The search for effective polymorphic markers in <i>Plasmodium vivax</i> is highly demanding to understand its transmission in a population. Due to the limited knowledge existing for <i>P. vivax</i>, the search for polymorphic ma...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2013-08-01
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Series: | Journal of Vector Borne Diseases |
Subjects: | |
Online Access: | http://www.mrcindia.org/journal/issues/503197.pdf |
Summary: | <b>Background & objectives:</b> The search for effective polymorphic markers in <i>Plasmodium vivax</i> is highly demanding to understand its transmission in a population. Due to the limited knowledge existing for <i>P. vivax</i>, the search for polymorphic markers for population studies is ongoing. The <i>MSP</i> gene family of <i>Plasmodia</i> has been linked with immune evasion. To study the circulating parasite population <i>P. vivax</i> merozoite surface protein 3β (<i>PvMSP3</i>β) polymorphic marker was used to investigate the genetic diversity of <i>P. vivax</i> in natural infections.
<b>Methods:</b> Polymorphism of <i>PvMSP3</i>β gene was determined in 46 <i>P. vivax</i> blood samples from six different regions of India by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) with <i>PstI</i>.
<b>Results:</b> Two different parasite genotypes, viz. type-A and type-B were detected among 46 samples that were positive for PCR, based on the size of the amplification. RFLP analysis with PstI showed 22 allelic groups and 15.2% samples revealed mixed infections on analysis.
<b>Conclusion:</b> PvMSP3β was found to be an effective molecular marker for <i>P. vivax</i> as it shows high diversity in India and multiple genotypes easily distinguishable without the need for sequencing. |
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ISSN: | 0972-9062 0972-9062 |