Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration
In this study, we investigated the effect of supplementing a non-dispersive dicalcium phosphate-rich calcium phosphate bone cement (DCP-rich CPC) with type I collagen on in vitro cellular activities and its performance as a bone graft material. Varying amounts of type I collagen were added during th...
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doaj-7fe7a2b8b08e4b359ec18d59f3c9b40c2020-11-25T00:56:09ZengMDPI AGMaterials1996-19442017-08-0110891010.3390/ma10080910ma10080910Incorporation of Collagen in Calcium Phosphate Cements for Controlling OsseointegrationMing-Hsien Hu0Pei-Yuan Lee1Wen-Cheng Chen2Jin-Jia Hu3Department of Biomedical Engineering, National Cheng Kung University, Tainan 701, TaiwanDepartment of Biomedical Engineering, National Cheng Kung University, Tainan 701, TaiwanDepartment of Fiber and Composite Materials, College of Engineering, Feng Chia University, Taichung 40724, TaiwanDepartment of Biomedical Engineering, National Cheng Kung University, Tainan 701, TaiwanIn this study, we investigated the effect of supplementing a non-dispersive dicalcium phosphate-rich calcium phosphate bone cement (DCP-rich CPC) with type I collagen on in vitro cellular activities and its performance as a bone graft material. Varying amounts of type I collagen were added during the preparation of the DCP-rich CPC. In vitro cell adhesion, morphology, viability, and alkaline phosphatase (ALP) activity were evaluated using progenitor bone cells. Bone graft performance was evaluated via a rat posterolateral lumbar fusion model and osteointegration of the implant. New bone formations in the restorative sites were assessed by micro-computed tomography (micro-CT) and histological analysis. We found that the incorporation of collagen into the DCP-rich CPC was associated with increased cell adhesion, cell viability, and ALP activity in vitro. The spinal fusion model revealed a significant increase in bone regeneration. Additionally, better osseointegration was observed between the host bone and graft with the DCP-rich CPC supplemented with collagen than with the collagen-free DCP-rich CPC control graft. Furthermore, compared to the control graft, the results of micro-CT showed that a smaller amount of residual material was observed with the collagen-containing DCP-rich CPC graft compared with the control graft, which suggests the collagen supplement enhanced new bone formation. Of the different mixtures evaluated in this study (0.8 g DCP-rich CPC supplemented with 0.1, 0.2, and 0.4 mL type I collagen, respectively), DCP-rich CPC supplemented with 0.4 mL collagen led to the highest level of osteogenesis. Our results suggest that the DCP-rich CPC supplemented with collagen has potential to be used as an effective bone graft material in spinal surgery.https://www.mdpi.com/1996-1944/10/8/910posterolateral lumbar fusionnon-dispersive calcium phosphate bone cementcollagenosteogenesis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ming-Hsien Hu Pei-Yuan Lee Wen-Cheng Chen Jin-Jia Hu |
spellingShingle |
Ming-Hsien Hu Pei-Yuan Lee Wen-Cheng Chen Jin-Jia Hu Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration Materials posterolateral lumbar fusion non-dispersive calcium phosphate bone cement collagen osteogenesis |
author_facet |
Ming-Hsien Hu Pei-Yuan Lee Wen-Cheng Chen Jin-Jia Hu |
author_sort |
Ming-Hsien Hu |
title |
Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration |
title_short |
Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration |
title_full |
Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration |
title_fullStr |
Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration |
title_full_unstemmed |
Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration |
title_sort |
incorporation of collagen in calcium phosphate cements for controlling osseointegration |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2017-08-01 |
description |
In this study, we investigated the effect of supplementing a non-dispersive dicalcium phosphate-rich calcium phosphate bone cement (DCP-rich CPC) with type I collagen on in vitro cellular activities and its performance as a bone graft material. Varying amounts of type I collagen were added during the preparation of the DCP-rich CPC. In vitro cell adhesion, morphology, viability, and alkaline phosphatase (ALP) activity were evaluated using progenitor bone cells. Bone graft performance was evaluated via a rat posterolateral lumbar fusion model and osteointegration of the implant. New bone formations in the restorative sites were assessed by micro-computed tomography (micro-CT) and histological analysis. We found that the incorporation of collagen into the DCP-rich CPC was associated with increased cell adhesion, cell viability, and ALP activity in vitro. The spinal fusion model revealed a significant increase in bone regeneration. Additionally, better osseointegration was observed between the host bone and graft with the DCP-rich CPC supplemented with collagen than with the collagen-free DCP-rich CPC control graft. Furthermore, compared to the control graft, the results of micro-CT showed that a smaller amount of residual material was observed with the collagen-containing DCP-rich CPC graft compared with the control graft, which suggests the collagen supplement enhanced new bone formation. Of the different mixtures evaluated in this study (0.8 g DCP-rich CPC supplemented with 0.1, 0.2, and 0.4 mL type I collagen, respectively), DCP-rich CPC supplemented with 0.4 mL collagen led to the highest level of osteogenesis. Our results suggest that the DCP-rich CPC supplemented with collagen has potential to be used as an effective bone graft material in spinal surgery. |
topic |
posterolateral lumbar fusion non-dispersive calcium phosphate bone cement collagen osteogenesis |
url |
https://www.mdpi.com/1996-1944/10/8/910 |
work_keys_str_mv |
AT minghsienhu incorporationofcollagenincalciumphosphatecementsforcontrollingosseointegration AT peiyuanlee incorporationofcollagenincalciumphosphatecementsforcontrollingosseointegration AT wenchengchen incorporationofcollagenincalciumphosphatecementsforcontrollingosseointegration AT jinjiahu incorporationofcollagenincalciumphosphatecementsforcontrollingosseointegration |
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