NMDA Receptor-Dependent Synaptic Activity in Dorsal Motor Nucleus of Vagus Mediates the Enhancement of Gastric Motility by Stimulating ST36
Previous studies have demonstrated the efficacy of electroacupuncture at ST36 for patients with gastrointestinal motility disorders. While several lines of evidence suggest that the effect may involve vagal reflex, the precise molecular mechanism underlying this process still remains unclear. Here w...
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doaj-7ff613079fbf4c4cb0dedcd5b34aaeda2020-11-24T22:30:25ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882012-01-01201210.1155/2012/438460438460NMDA Receptor-Dependent Synaptic Activity in Dorsal Motor Nucleus of Vagus Mediates the Enhancement of Gastric Motility by Stimulating ST36Xinyan Gao0Yongfa Qiao1Baohui Jia2Xianghong Jing3Bin Cheng4Lei Wen5Qiwen Tan6Yi Zhou7Bing Zhu8Haifa Qiao9Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, 16 Nanxiaojie Street, Dongzhimennei, Beijing 100700, ChinaQingdao Haici Medical Group, 4 Renmin Road, Qingdao 266033, ChinaGuanganmen Hospitals, China Academy of Chinese Medical Science, Beijing 100053, ChinaInstitute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, 16 Nanxiaojie Street, Dongzhimennei, Beijing 100700, ChinaThe Affiliated Hospital, Shandong University of Traditional Chinese Medicine, Jinan 250014, ChinaDepartment of Pharmacology, Southern Medical University, Guangzhou 510515, ChinaThe Affiliated Hospital, Shandong University of Traditional Chinese Medicine, Jinan 250014, ChinaDepartment of Biomedical Sciences, Florida State University College of Medicine, Tallahassee, FL 32306, USAInstitute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, 16 Nanxiaojie Street, Dongzhimennei, Beijing 100700, ChinaInstitute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, 16 Nanxiaojie Street, Dongzhimennei, Beijing 100700, ChinaPrevious studies have demonstrated the efficacy of electroacupuncture at ST36 for patients with gastrointestinal motility disorders. While several lines of evidence suggest that the effect may involve vagal reflex, the precise molecular mechanism underlying this process still remains unclear. Here we report that the intragastric pressure increase induced by low frequency electric stimulation at ST36 was blocked by AP-5, an antagonist of N-methyl-D-aspartate receptors (NMDARs). Indeed, stimulating ST36 enhanced NMDAR-mediated, but not 2-amino-3-(5-methyl-3-oxo-1,2-oxazol-4-yl)propanoic-acid-(AMPA-) receptor-(AMPAR-) mediated synaptic transmission in gastric-projecting neurons of the dorsal motor nucleus of the vagus (DMV). We also identified that suppression of presynaptic μ-opioid receptors may contribute to upregulation of NMDAR-mediated synaptic transmission induced by electroacupuncture at ST36. Furthermore, we determined that the glutamate-receptor-2a-(NR2A-) containing NMDARs are essential for NMDAR-mediated enhancement of gastric motility caused by stimulating ST36. Taken together, our results reveal an important role of NMDA receptors in mediating enhancement of gastric motility induced by stimulating ST36.http://dx.doi.org/10.1155/2012/438460 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xinyan Gao Yongfa Qiao Baohui Jia Xianghong Jing Bin Cheng Lei Wen Qiwen Tan Yi Zhou Bing Zhu Haifa Qiao |
spellingShingle |
Xinyan Gao Yongfa Qiao Baohui Jia Xianghong Jing Bin Cheng Lei Wen Qiwen Tan Yi Zhou Bing Zhu Haifa Qiao NMDA Receptor-Dependent Synaptic Activity in Dorsal Motor Nucleus of Vagus Mediates the Enhancement of Gastric Motility by Stimulating ST36 Evidence-Based Complementary and Alternative Medicine |
author_facet |
Xinyan Gao Yongfa Qiao Baohui Jia Xianghong Jing Bin Cheng Lei Wen Qiwen Tan Yi Zhou Bing Zhu Haifa Qiao |
author_sort |
Xinyan Gao |
title |
NMDA Receptor-Dependent Synaptic Activity in Dorsal Motor Nucleus of Vagus Mediates the Enhancement of Gastric Motility by Stimulating ST36 |
title_short |
NMDA Receptor-Dependent Synaptic Activity in Dorsal Motor Nucleus of Vagus Mediates the Enhancement of Gastric Motility by Stimulating ST36 |
title_full |
NMDA Receptor-Dependent Synaptic Activity in Dorsal Motor Nucleus of Vagus Mediates the Enhancement of Gastric Motility by Stimulating ST36 |
title_fullStr |
NMDA Receptor-Dependent Synaptic Activity in Dorsal Motor Nucleus of Vagus Mediates the Enhancement of Gastric Motility by Stimulating ST36 |
title_full_unstemmed |
NMDA Receptor-Dependent Synaptic Activity in Dorsal Motor Nucleus of Vagus Mediates the Enhancement of Gastric Motility by Stimulating ST36 |
title_sort |
nmda receptor-dependent synaptic activity in dorsal motor nucleus of vagus mediates the enhancement of gastric motility by stimulating st36 |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2012-01-01 |
description |
Previous studies have demonstrated the efficacy of electroacupuncture at ST36 for patients with gastrointestinal motility disorders. While several lines of evidence suggest that the effect may involve vagal reflex, the precise molecular mechanism underlying this process still remains unclear. Here we report that the intragastric pressure increase induced by low frequency electric stimulation at ST36 was blocked by AP-5, an antagonist of N-methyl-D-aspartate receptors (NMDARs). Indeed, stimulating ST36 enhanced NMDAR-mediated, but not 2-amino-3-(5-methyl-3-oxo-1,2-oxazol-4-yl)propanoic-acid-(AMPA-) receptor-(AMPAR-) mediated synaptic transmission in gastric-projecting neurons of the dorsal motor nucleus of the vagus (DMV). We also identified that suppression of presynaptic μ-opioid receptors may contribute to upregulation of NMDAR-mediated synaptic transmission induced by electroacupuncture at ST36. Furthermore, we determined that the glutamate-receptor-2a-(NR2A-) containing NMDARs are essential for NMDAR-mediated enhancement of gastric motility caused by stimulating ST36. Taken together, our results reveal an important role of NMDA receptors in mediating enhancement of gastric motility induced by stimulating ST36. |
url |
http://dx.doi.org/10.1155/2012/438460 |
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