Antimicrobial Carvacrol Incorporated in Flaxseed Gum-Sodium Alginate Active Films to Improve the Quality Attributes of Chinese Sea bass (Lateolabrax maculatus) during Cold Storage
The objective of this research was to explore the antimicrobial activity and mechanism of carvacrol against <i>Vibrio Parahemolyticus</i>, <i>Shewanella putrefaciens</i>, <i>Staphylococcus aureus</i> and <i>Pseudomonas fluorescens</i> and evaluate the...
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doaj-8022039045dc401f99fda79e5177d4ca2020-11-25T00:59:16ZengMDPI AGMolecules1420-30492019-09-012418329210.3390/molecules24183292molecules24183292Antimicrobial Carvacrol Incorporated in Flaxseed Gum-Sodium Alginate Active Films to Improve the Quality Attributes of Chinese Sea bass (Lateolabrax maculatus) during Cold StorageShiyuan Fang0Qianqian Zhou1Yan Hu2Feng Liu3Jun Mei4Jing Xie5College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaThe objective of this research was to explore the antimicrobial activity and mechanism of carvacrol against <i>Vibrio Parahemolyticus</i>, <i>Shewanella putrefaciens</i>, <i>Staphylococcus aureus</i> and <i>Pseudomonas fluorescens</i> and evaluate the effect of the addition of carvacrol/β-cyclodextrin emulsions to flaxseed gum (FSG)-sodium alginate (SA) edible films on the preservation of Chinese sea bass (<i>Lateolabrax maculatus</i>) fillets during refrigerated storage. The minimum inhibitory concentration (MIC) of carvacrol against <i>V. parahemolyticus</i>, <i>S. putrefaciens</i>, <i>S. aureus</i> and <i>P. fluorescens</i> were 0.5, 0.5, 0.125, and 0.5 mg/mL, respectively. Alkaline phosphatase activity assay, nucleotide and protein leakage, and scanning electron microscope demonstrated that carvacrol damaged the external structure of the tested bacterial cells causing leakage of cytoplasmic components. At the same time, when FSG-SA films containing carvacrol used as coating agents for Chinese sea bass fillets cold storage, FSG-SA films containing 1.0 or 2.0 mg/mL carvacrol could significantly reduce TVB-N content, K-value, the degree of microbial deterioration and maintain quality of sea bass fillets according to organoleptic evaluation results.https://www.mdpi.com/1420-3049/24/18/3292carvacrolantimicrobial activityChinese sea bassactive coatingshelf life |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shiyuan Fang Qianqian Zhou Yan Hu Feng Liu Jun Mei Jing Xie |
spellingShingle |
Shiyuan Fang Qianqian Zhou Yan Hu Feng Liu Jun Mei Jing Xie Antimicrobial Carvacrol Incorporated in Flaxseed Gum-Sodium Alginate Active Films to Improve the Quality Attributes of Chinese Sea bass (Lateolabrax maculatus) during Cold Storage Molecules carvacrol antimicrobial activity Chinese sea bass active coating shelf life |
author_facet |
Shiyuan Fang Qianqian Zhou Yan Hu Feng Liu Jun Mei Jing Xie |
author_sort |
Shiyuan Fang |
title |
Antimicrobial Carvacrol Incorporated in Flaxseed Gum-Sodium Alginate Active Films to Improve the Quality Attributes of Chinese Sea bass (Lateolabrax maculatus) during Cold Storage |
title_short |
Antimicrobial Carvacrol Incorporated in Flaxseed Gum-Sodium Alginate Active Films to Improve the Quality Attributes of Chinese Sea bass (Lateolabrax maculatus) during Cold Storage |
title_full |
Antimicrobial Carvacrol Incorporated in Flaxseed Gum-Sodium Alginate Active Films to Improve the Quality Attributes of Chinese Sea bass (Lateolabrax maculatus) during Cold Storage |
title_fullStr |
Antimicrobial Carvacrol Incorporated in Flaxseed Gum-Sodium Alginate Active Films to Improve the Quality Attributes of Chinese Sea bass (Lateolabrax maculatus) during Cold Storage |
title_full_unstemmed |
Antimicrobial Carvacrol Incorporated in Flaxseed Gum-Sodium Alginate Active Films to Improve the Quality Attributes of Chinese Sea bass (Lateolabrax maculatus) during Cold Storage |
title_sort |
antimicrobial carvacrol incorporated in flaxseed gum-sodium alginate active films to improve the quality attributes of chinese sea bass (lateolabrax maculatus) during cold storage |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2019-09-01 |
description |
The objective of this research was to explore the antimicrobial activity and mechanism of carvacrol against <i>Vibrio Parahemolyticus</i>, <i>Shewanella putrefaciens</i>, <i>Staphylococcus aureus</i> and <i>Pseudomonas fluorescens</i> and evaluate the effect of the addition of carvacrol/β-cyclodextrin emulsions to flaxseed gum (FSG)-sodium alginate (SA) edible films on the preservation of Chinese sea bass (<i>Lateolabrax maculatus</i>) fillets during refrigerated storage. The minimum inhibitory concentration (MIC) of carvacrol against <i>V. parahemolyticus</i>, <i>S. putrefaciens</i>, <i>S. aureus</i> and <i>P. fluorescens</i> were 0.5, 0.5, 0.125, and 0.5 mg/mL, respectively. Alkaline phosphatase activity assay, nucleotide and protein leakage, and scanning electron microscope demonstrated that carvacrol damaged the external structure of the tested bacterial cells causing leakage of cytoplasmic components. At the same time, when FSG-SA films containing carvacrol used as coating agents for Chinese sea bass fillets cold storage, FSG-SA films containing 1.0 or 2.0 mg/mL carvacrol could significantly reduce TVB-N content, K-value, the degree of microbial deterioration and maintain quality of sea bass fillets according to organoleptic evaluation results. |
topic |
carvacrol antimicrobial activity Chinese sea bass active coating shelf life |
url |
https://www.mdpi.com/1420-3049/24/18/3292 |
work_keys_str_mv |
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