A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement
Winter oilseed rape is characterized by a low N use efficiency related to a weak leaf N remobilization efficiency (NRE) at vegetative stages. By investigating the natural genotypic variability of leaf NRE, our goal was to characterize the relevant physiological traits and the main protease classes a...
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doaj-802665e6f86a4c0db59ab4ffaff118a92020-11-24T21:03:01ZengMDPI AGPlants2223-77472015-12-0151110.3390/plants5010001plants5010001A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE ImprovementAlexandra Girondé0Marine Poret1Philippe Etienne2Jacques Trouverie3Alain Bouchereau4Françoise Le Cahérec5Laurent Leport6Marie-Françoise Niogret7Jean-Christophe Avice8UMR INRA-UCBN 950 Ecophysiologie Végétale, Agronomie & Nutritions N.C.S., Université de Caen Normandie, F-14032 Caen, FranceUMR INRA-UCBN 950 Ecophysiologie Végétale, Agronomie & Nutritions N.C.S., Université de Caen Normandie, F-14032 Caen, FranceUMR INRA-UCBN 950 Ecophysiologie Végétale, Agronomie & Nutritions N.C.S., Université de Caen Normandie, F-14032 Caen, FranceUMR INRA-UCBN 950 Ecophysiologie Végétale, Agronomie & Nutritions N.C.S., Université de Caen Normandie, F-14032 Caen, FranceINRA, UMR 1349 Institut de Génétique, Environnement et Protection des Plantes, Agrocampus Ouest, Université de Rennes 1, F-35653 Le Rheu, FranceINRA, UMR 1349 Institut de Génétique, Environnement et Protection des Plantes, Agrocampus Ouest, Université de Rennes 1, F-35653 Le Rheu, FranceINRA, UMR 1349 Institut de Génétique, Environnement et Protection des Plantes, Agrocampus Ouest, Université de Rennes 1, F-35653 Le Rheu, FranceINRA, UMR 1349 Institut de Génétique, Environnement et Protection des Plantes, Agrocampus Ouest, Université de Rennes 1, F-35653 Le Rheu, FranceUMR INRA-UCBN 950 Ecophysiologie Végétale, Agronomie & Nutritions N.C.S., Université de Caen Normandie, F-14032 Caen, FranceWinter oilseed rape is characterized by a low N use efficiency related to a weak leaf N remobilization efficiency (NRE) at vegetative stages. By investigating the natural genotypic variability of leaf NRE, our goal was to characterize the relevant physiological traits and the main protease classes associated with an efficient proteolysis and high leaf NRE in response to ample or restricted nitrate supply. The degradation rate of soluble proteins and D1 protein (a thylakoid-bound protein) were correlated to N remobilization, except for the genotype Samouraï which showed a low NRE despite high levels of proteolysis. Under restricted nitrate conditions, high levels of soluble protein degradation were associated with serine, cysteine and aspartic proteases at acidic pH. Low leaf NRE was related to a weak proteolysis of both soluble and thylakoid-bound proteins. The results obtained on the genotype Samouraï suggest that the timing between the onset of proteolysis and abscission could be a determinant. The specific involvement of acidic proteases suggests that autophagy and/or senescence-associated vacuoles are implicated in N remobilization under low N conditions. The data revealed that the rate of D1 degradation could be a relevant indicator of leaf NRE and might be used as a tool for plant breeding.http://www.mdpi.com/2223-7747/5/1/1Brassica napusN remobilizationgenotypic variabilityproteolysisacidic proteases |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alexandra Girondé Marine Poret Philippe Etienne Jacques Trouverie Alain Bouchereau Françoise Le Cahérec Laurent Leport Marie-Françoise Niogret Jean-Christophe Avice |
spellingShingle |
Alexandra Girondé Marine Poret Philippe Etienne Jacques Trouverie Alain Bouchereau Françoise Le Cahérec Laurent Leport Marie-Françoise Niogret Jean-Christophe Avice A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement Plants Brassica napus N remobilization genotypic variability proteolysis acidic proteases |
author_facet |
Alexandra Girondé Marine Poret Philippe Etienne Jacques Trouverie Alain Bouchereau Françoise Le Cahérec Laurent Leport Marie-Françoise Niogret Jean-Christophe Avice |
author_sort |
Alexandra Girondé |
title |
A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement |
title_short |
A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement |
title_full |
A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement |
title_fullStr |
A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement |
title_full_unstemmed |
A Comparative Study of Proteolytic Mechanisms during Leaf Senescence of Four Genotypes of Winter Oilseed Rape Highlighted Relevant Physiological and Molecular Traits for NRE Improvement |
title_sort |
comparative study of proteolytic mechanisms during leaf senescence of four genotypes of winter oilseed rape highlighted relevant physiological and molecular traits for nre improvement |
publisher |
MDPI AG |
series |
Plants |
issn |
2223-7747 |
publishDate |
2015-12-01 |
description |
Winter oilseed rape is characterized by a low N use efficiency related to a weak leaf N remobilization efficiency (NRE) at vegetative stages. By investigating the natural genotypic variability of leaf NRE, our goal was to characterize the relevant physiological traits and the main protease classes associated with an efficient proteolysis and high leaf NRE in response to ample or restricted nitrate supply. The degradation rate of soluble proteins and D1 protein (a thylakoid-bound protein) were correlated to N remobilization, except for the genotype Samouraï which showed a low NRE despite high levels of proteolysis. Under restricted nitrate conditions, high levels of soluble protein degradation were associated with serine, cysteine and aspartic proteases at acidic pH. Low leaf NRE was related to a weak proteolysis of both soluble and thylakoid-bound proteins. The results obtained on the genotype Samouraï suggest that the timing between the onset of proteolysis and abscission could be a determinant. The specific involvement of acidic proteases suggests that autophagy and/or senescence-associated vacuoles are implicated in N remobilization under low N conditions. The data revealed that the rate of D1 degradation could be a relevant indicator of leaf NRE and might be used as a tool for plant breeding. |
topic |
Brassica napus N remobilization genotypic variability proteolysis acidic proteases |
url |
http://www.mdpi.com/2223-7747/5/1/1 |
work_keys_str_mv |
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