Mass Production of Early-Stage Bone-Marrow-Derived Mesenchymal Stem Cells of Rat Using Gelatin-Coated Matrix
Although preparation of early-stage bone-marrow-derived mesenchymal stem cells (BM-MSCs) is critical for successful cell transplantation therapy, no culture system offers a sufficient number of early-stage BM-MSCs for cell transplantation. Accordingly, we developed a culture system capable of produc...
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doaj-80318b04c73a44729075f758010993d22020-11-25T00:48:04ZengHindawi LimitedBioMed Research International2314-61332314-61412013-01-01201310.1155/2013/347618347618Mass Production of Early-Stage Bone-Marrow-Derived Mesenchymal Stem Cells of Rat Using Gelatin-Coated MatrixYoung Hyun Park0Jung Im Yun1Na Rae Han2Hye Jin Park3Ji Yeon Ahn4Choonghyo Kim5Jung Hoon Choi6Eunsong Lee7Jeong Mook Lim8Seung Tae Lee9Stem Cell and Bioevaluation, WCU Biomodulation Program, Seoul National University, Seoul 151-921, Republic of KoreaDepartment of Agricultural Biotechnology, College of Agricultural Life and Science, Seoul National University, Seoul 151-921, Republic of KoreaDepartment of Animal Biotechnology, College of Animal Life Science, Kangwon National University, Chuncheon 200-701, Republic of KoreaDepartment of Animal Biotechnology, College of Animal Life Science, Kangwon National University, Chuncheon 200-701, Republic of KoreaCancer Research Institute, College of Medicine, Seoul National University, Seoul 110-799, Republic of KoreaDepartment of Neurosurgery, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon 200-701, Republic of KoreaCollege of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 200-701, Republic of KoreaCollege of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 200-701, Republic of KoreaStem Cell and Bioevaluation, WCU Biomodulation Program, Seoul National University, Seoul 151-921, Republic of KoreaDepartment of Animal Biotechnology, College of Animal Life Science, Kangwon National University, Chuncheon 200-701, Republic of KoreaAlthough preparation of early-stage bone-marrow-derived mesenchymal stem cells (BM-MSCs) is critical for successful cell transplantation therapy, no culture system offers a sufficient number of early-stage BM-MSCs for cell transplantation. Accordingly, we developed a culture system capable of producing a large number of early-stage BM-MSCs by using gelatin-coated matrix. The greatest retrieval and proliferation rates of the earliest-stage rat BM-MSCs were detected in bone-marrow-derived cells cultured on 1% (wt/v) gelatin-coated matrix, which showed significantly greater colony forming unit-fibroblast number, diameter, and total cell number. Moreover, continuous culture of the earliest-stage BM-MSCs on 1% (wt/v) gelatin-coated matrix resulted in a maximum of 21.2±2.7 fold increase in the cumulative total number of early-stage BM-MSCs at passage 5. BM-MSCs generated in large quantities due to a reduced doubling time and an increased yield of cell population in S/G2/M phase showed typical fibroblast-like morphology and no significant differences in BM-MSC-related surface marker expression and differentiation potential, except for an increased ratio of differentiation into a neurogenic lineage. The use of gelatin-coated matrix in the retrieval and culture of BM-MSCs contributes greatly to the effective isolation and mass production of early-stage BM-MSCs.http://dx.doi.org/10.1155/2013/347618 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Young Hyun Park Jung Im Yun Na Rae Han Hye Jin Park Ji Yeon Ahn Choonghyo Kim Jung Hoon Choi Eunsong Lee Jeong Mook Lim Seung Tae Lee |
spellingShingle |
Young Hyun Park Jung Im Yun Na Rae Han Hye Jin Park Ji Yeon Ahn Choonghyo Kim Jung Hoon Choi Eunsong Lee Jeong Mook Lim Seung Tae Lee Mass Production of Early-Stage Bone-Marrow-Derived Mesenchymal Stem Cells of Rat Using Gelatin-Coated Matrix BioMed Research International |
author_facet |
Young Hyun Park Jung Im Yun Na Rae Han Hye Jin Park Ji Yeon Ahn Choonghyo Kim Jung Hoon Choi Eunsong Lee Jeong Mook Lim Seung Tae Lee |
author_sort |
Young Hyun Park |
title |
Mass Production of Early-Stage Bone-Marrow-Derived Mesenchymal Stem Cells of Rat Using Gelatin-Coated Matrix |
title_short |
Mass Production of Early-Stage Bone-Marrow-Derived Mesenchymal Stem Cells of Rat Using Gelatin-Coated Matrix |
title_full |
Mass Production of Early-Stage Bone-Marrow-Derived Mesenchymal Stem Cells of Rat Using Gelatin-Coated Matrix |
title_fullStr |
Mass Production of Early-Stage Bone-Marrow-Derived Mesenchymal Stem Cells of Rat Using Gelatin-Coated Matrix |
title_full_unstemmed |
Mass Production of Early-Stage Bone-Marrow-Derived Mesenchymal Stem Cells of Rat Using Gelatin-Coated Matrix |
title_sort |
mass production of early-stage bone-marrow-derived mesenchymal stem cells of rat using gelatin-coated matrix |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2013-01-01 |
description |
Although preparation of early-stage bone-marrow-derived mesenchymal stem cells (BM-MSCs) is critical for successful cell transplantation therapy, no culture system offers a sufficient number of early-stage BM-MSCs for cell transplantation. Accordingly, we developed a culture system capable of producing a large number of early-stage BM-MSCs by using gelatin-coated matrix. The greatest retrieval and proliferation rates of the earliest-stage rat BM-MSCs were detected in bone-marrow-derived cells cultured on 1% (wt/v) gelatin-coated matrix, which showed significantly greater colony forming unit-fibroblast number, diameter, and total cell number. Moreover, continuous culture of the earliest-stage BM-MSCs on 1% (wt/v) gelatin-coated matrix resulted in a maximum of 21.2±2.7 fold increase in the cumulative total number of early-stage BM-MSCs at passage 5. BM-MSCs generated in large quantities due to a reduced doubling time and an increased yield of cell population in S/G2/M phase showed typical fibroblast-like morphology and no significant differences in BM-MSC-related surface marker expression and differentiation potential, except for an increased ratio of differentiation into a neurogenic lineage. The use of gelatin-coated matrix in the retrieval and culture of BM-MSCs contributes greatly to the effective isolation and mass production of early-stage BM-MSCs. |
url |
http://dx.doi.org/10.1155/2013/347618 |
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