Circ_0015756 promotes the progression of ovarian cancer by regulating miR-942-5p/CUL4B pathway
Abstract Background Ovarian cancer (OC) is the gynecologic cancer with the highest mortality. Circular RNAs (circRNAs) play a vital role in the development and progression of cancer. This study aimed to explore the potential role of circ_0015756 in OC and its molecular mechanism. Methods The levels...
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doaj-803918ba52a5408288ab91e9afd4641d2020-11-29T12:10:22ZengBMCCancer Cell International1475-28672020-11-0120111310.1186/s12935-020-01666-1Circ_0015756 promotes the progression of ovarian cancer by regulating miR-942-5p/CUL4B pathwayZhenhua Du0Lei Wang1Yu Xia2Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical UniversityDepartment of Obstetrics and Gynecology, Shengjing Hospital of China Medical UniversityDepartment of Obstetrics and Gynecology, Shengjing Hospital of China Medical UniversityAbstract Background Ovarian cancer (OC) is the gynecologic cancer with the highest mortality. Circular RNAs (circRNAs) play a vital role in the development and progression of cancer. This study aimed to explore the potential role of circ_0015756 in OC and its molecular mechanism. Methods The levels of circ_0015756, microRNA-942-5p (miR-942-5p) and Cullin 4B (CUL4B) were determined by quantitative real-time PCR (qRT-PCR) or Western blot assay. Cell proliferation, apoptosis, migration and invasion were assessed by Cell Counting Kit-8 (CCK-8), colony formation assay, flow cytometry and transwell assay. The levels of proliferation-related and metastasis-related proteins were measured by Western blot assay. The relationship between miR-942-5p and circ_0015756 or CUL4B was verified by dual-luciferase reporter assay, RNA immunoprecipitation assay and RNA pull-down assay. Xenograft assay was used to analyze tumor growth in vivo. Results Circ_0015756 and CUL4B levels were increased, while miR-942-5p level was decreased in OC tissues and cells. Depletion of circ_0015756 suppressed proliferation, migration and invasion and promoted apoptosis in OC cells. Down-regulation of circ_0015756 hindered OC cell progression via modulating miR-942-5p. Also, up-regulation of miR-942-5p impeded OC cell development by targeting CUL4B. Mechanistically, circ_0015756 up-regulated CUL4B via sponging miR-942-5p. Moreover, circ_0015756 silencing inhibited tumor growth in vivo. Conclusion Knockdown of circ_0015756 suppressed OC progression via regulating miR-942-5p/CUL4B axis, suggesting that circ_0015756 might be a potential therapeutic target for ovarian cancer.https://doi.org/10.1186/s12935-020-01666-1Ovarian cancerCirc_0015756miR-942-5pCUL4B |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhenhua Du Lei Wang Yu Xia |
spellingShingle |
Zhenhua Du Lei Wang Yu Xia Circ_0015756 promotes the progression of ovarian cancer by regulating miR-942-5p/CUL4B pathway Cancer Cell International Ovarian cancer Circ_0015756 miR-942-5p CUL4B |
author_facet |
Zhenhua Du Lei Wang Yu Xia |
author_sort |
Zhenhua Du |
title |
Circ_0015756 promotes the progression of ovarian cancer by regulating miR-942-5p/CUL4B pathway |
title_short |
Circ_0015756 promotes the progression of ovarian cancer by regulating miR-942-5p/CUL4B pathway |
title_full |
Circ_0015756 promotes the progression of ovarian cancer by regulating miR-942-5p/CUL4B pathway |
title_fullStr |
Circ_0015756 promotes the progression of ovarian cancer by regulating miR-942-5p/CUL4B pathway |
title_full_unstemmed |
Circ_0015756 promotes the progression of ovarian cancer by regulating miR-942-5p/CUL4B pathway |
title_sort |
circ_0015756 promotes the progression of ovarian cancer by regulating mir-942-5p/cul4b pathway |
publisher |
BMC |
series |
Cancer Cell International |
issn |
1475-2867 |
publishDate |
2020-11-01 |
description |
Abstract Background Ovarian cancer (OC) is the gynecologic cancer with the highest mortality. Circular RNAs (circRNAs) play a vital role in the development and progression of cancer. This study aimed to explore the potential role of circ_0015756 in OC and its molecular mechanism. Methods The levels of circ_0015756, microRNA-942-5p (miR-942-5p) and Cullin 4B (CUL4B) were determined by quantitative real-time PCR (qRT-PCR) or Western blot assay. Cell proliferation, apoptosis, migration and invasion were assessed by Cell Counting Kit-8 (CCK-8), colony formation assay, flow cytometry and transwell assay. The levels of proliferation-related and metastasis-related proteins were measured by Western blot assay. The relationship between miR-942-5p and circ_0015756 or CUL4B was verified by dual-luciferase reporter assay, RNA immunoprecipitation assay and RNA pull-down assay. Xenograft assay was used to analyze tumor growth in vivo. Results Circ_0015756 and CUL4B levels were increased, while miR-942-5p level was decreased in OC tissues and cells. Depletion of circ_0015756 suppressed proliferation, migration and invasion and promoted apoptosis in OC cells. Down-regulation of circ_0015756 hindered OC cell progression via modulating miR-942-5p. Also, up-regulation of miR-942-5p impeded OC cell development by targeting CUL4B. Mechanistically, circ_0015756 up-regulated CUL4B via sponging miR-942-5p. Moreover, circ_0015756 silencing inhibited tumor growth in vivo. Conclusion Knockdown of circ_0015756 suppressed OC progression via regulating miR-942-5p/CUL4B axis, suggesting that circ_0015756 might be a potential therapeutic target for ovarian cancer. |
topic |
Ovarian cancer Circ_0015756 miR-942-5p CUL4B |
url |
https://doi.org/10.1186/s12935-020-01666-1 |
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