Generation of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiation

Generation of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiation

Caudal-type homeobox 2 (CDX2) transcription factor is an important marker for early trophoblast lineages and intestinal epithelium. Due to its nuclear expression the immunostaining and sorting of viable CDX2pos cells is not possible. In this paper we report the generation and describe key characteri...

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Main Authors: Svitlana V. Malysheva, Stephanie Wunderlich, Alexandra Haase, Gudrun Göhring, Ulrich Martin, Sylvia Merkert
Format: Article
Language:English
Published: Elsevier 2018-07-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506118301338
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spelling doaj-804cd8dd84364e3b8384aa036fb8d42c2020-11-24T23:59:51ZengElsevierStem Cell Research1873-50612018-07-0130117121Generation of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiationSvitlana V. Malysheva0Stephanie Wunderlich1Alexandra Haase2Gudrun Göhring3Ulrich Martin4Sylvia Merkert5Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, GermanyREBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany; Department of Human Genetics, Hannover Medical School, 30625 Hannover, GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, 30625 Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, 30625 Hannover, Germany; Corresponding author at: Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, 30625 Hannover, Germany.Caudal-type homeobox 2 (CDX2) transcription factor is an important marker for early trophoblast lineages and intestinal epithelium. Due to its nuclear expression the immunostaining and sorting of viable CDX2pos cells is not possible. In this paper we report the generation and describe key characteristics of a CDX2Venus knock-in reporter hiPSC-cell line (MHHi007-A-1) which can serve as an in vitro tool to study human trophoblast and intestinal differentiation.http://www.sciencedirect.com/science/article/pii/S1873506118301338
collection DOAJ
language English
format Article
sources DOAJ
author Svitlana V. Malysheva
Stephanie Wunderlich
Alexandra Haase
Gudrun Göhring
Ulrich Martin
Sylvia Merkert
spellingShingle Svitlana V. Malysheva
Stephanie Wunderlich
Alexandra Haase
Gudrun Göhring
Ulrich Martin
Sylvia Merkert
Generation of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiation
Stem Cell Research
author_facet Svitlana V. Malysheva
Stephanie Wunderlich
Alexandra Haase
Gudrun Göhring
Ulrich Martin
Sylvia Merkert
author_sort Svitlana V. Malysheva
title Generation of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiation
title_short Generation of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiation
title_full Generation of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiation
title_fullStr Generation of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiation
title_full_unstemmed Generation of a human CDX2 knock-in reporter iPSC line (MHHi007-A-1) to model human trophoblast differentiation
title_sort generation of a human cdx2 knock-in reporter ipsc line (mhhi007-a-1) to model human trophoblast differentiation
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2018-07-01
description Caudal-type homeobox 2 (CDX2) transcription factor is an important marker for early trophoblast lineages and intestinal epithelium. Due to its nuclear expression the immunostaining and sorting of viable CDX2pos cells is not possible. In this paper we report the generation and describe key characteristics of a CDX2Venus knock-in reporter hiPSC-cell line (MHHi007-A-1) which can serve as an in vitro tool to study human trophoblast and intestinal differentiation.
url http://www.sciencedirect.com/science/article/pii/S1873506118301338
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AT stephaniewunderlich generationofahumancdx2knockinreporteripsclinemhhi007a1tomodelhumantrophoblastdifferentiation
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