Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation
Radiation-induced genomic instability refers to a type of damage transmitted over many generations following irradiation. This delayed impact of radiation exposure may pose a high risk to human health and increases concern over the dose limit of radiation exposure for both the public and radiation w...
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doaj-807632dacdff4eb2b436210a8236716e2020-11-24T22:32:08ZengMDPI AGInternational Journal of Environmental Research and Public Health1660-46012015-12-011212151621517210.3390/ijerph121214979ijerph121214979Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of RadiationYoon Hee Cho0Su Young Kim1Hae Dong Woo2Yang Jee Kim3Sung Whan Ha4Hai Won Chung5Department of Molecular Epidemiology, School of Public Health, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, KoreaDepartment of Preventive Medicine, School of Medicine, Jeju National University, 66 Jejudaehakno, Jeju-si, Jeju-do 690-756, KoreaMolecular Epidemiology Branch, Division of Cancer Epidemiology and Prevention, Research Institute, National Cancer Center, Goyang-si, Gyeonggi-do 410-769, KoreaDa Vinci College of General Education, Chung-ang University, 84 HeukSeok-Ro, DongJak-gu, Seoul 156-756, KoreaDepartment of Radiation Oncology, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 110-744, KoreaDepartment of Molecular Epidemiology, School of Public Health, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, KoreaRadiation-induced genomic instability refers to a type of damage transmitted over many generations following irradiation. This delayed impact of radiation exposure may pose a high risk to human health and increases concern over the dose limit of radiation exposure for both the public and radiation workers. Therefore, the development of additional biomarkers is still needed for the detection of delayed responses following low doses of radiation exposure. In this study, we examined the effect of X-irradiation on delayed induction of numerical chromosomal aberrations in normal human fibroblasts irradiated with 20, 50 and 100 cGy of X-rays using the micronucleus-centromere assay. Frequencies of centromere negative- and positive-micronuclei, and aneuploidy of chromosome 1 and 4 were analyzed in the surviving cells at 28, 88 and 240 h after X-irradiation. X-irradiation increased the frequency of micronuclei (MN) in a dose-dependent manner in the cells at all measured time-points, but no significant differences in MN frequency among cell passages were observed. Aneuploid frequency of chromosomes 1 and 4 increased with radiation doses, and a significantly higher frequency of aneuploidy was observed in the surviving cells analyzed at 240 h compared to 28 h. These results indicate that low-dose of X-irradiation can induce delayed aneuploidy of chromosomes 1 and 4 in normal fibroblasts.http://www.mdpi.com/1660-4601/12/12/14979radiation-induced genomic instabilityX-irradiationmicronucleus-centromere assaymicronucleianeuploidy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yoon Hee Cho Su Young Kim Hae Dong Woo Yang Jee Kim Sung Whan Ha Hai Won Chung |
spellingShingle |
Yoon Hee Cho Su Young Kim Hae Dong Woo Yang Jee Kim Sung Whan Ha Hai Won Chung Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation International Journal of Environmental Research and Public Health radiation-induced genomic instability X-irradiation micronucleus-centromere assay micronuclei aneuploidy |
author_facet |
Yoon Hee Cho Su Young Kim Hae Dong Woo Yang Jee Kim Sung Whan Ha Hai Won Chung |
author_sort |
Yoon Hee Cho |
title |
Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation |
title_short |
Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation |
title_full |
Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation |
title_fullStr |
Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation |
title_full_unstemmed |
Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation |
title_sort |
delayed numerical chromosome aberrations in human fibroblasts by low dose of radiation |
publisher |
MDPI AG |
series |
International Journal of Environmental Research and Public Health |
issn |
1660-4601 |
publishDate |
2015-12-01 |
description |
Radiation-induced genomic instability refers to a type of damage transmitted over many generations following irradiation. This delayed impact of radiation exposure may pose a high risk to human health and increases concern over the dose limit of radiation exposure for both the public and radiation workers. Therefore, the development of additional biomarkers is still needed for the detection of delayed responses following low doses of radiation exposure. In this study, we examined the effect of X-irradiation on delayed induction of numerical chromosomal aberrations in normal human fibroblasts irradiated with 20, 50 and 100 cGy of X-rays using the micronucleus-centromere assay. Frequencies of centromere negative- and positive-micronuclei, and aneuploidy of chromosome 1 and 4 were analyzed in the surviving cells at 28, 88 and 240 h after X-irradiation. X-irradiation increased the frequency of micronuclei (MN) in a dose-dependent manner in the cells at all measured time-points, but no significant differences in MN frequency among cell passages were observed. Aneuploid frequency of chromosomes 1 and 4 increased with radiation doses, and a significantly higher frequency of aneuploidy was observed in the surviving cells analyzed at 240 h compared to 28 h. These results indicate that low-dose of X-irradiation can induce delayed aneuploidy of chromosomes 1 and 4 in normal fibroblasts. |
topic |
radiation-induced genomic instability X-irradiation micronucleus-centromere assay micronuclei aneuploidy |
url |
http://www.mdpi.com/1660-4601/12/12/14979 |
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