Vitamin D Attenuates Oxidative Damage and Inflammation in Retinal Pigment Epithelial Cells
Age-related macular degeneration (AMD), the most common visual disorder in elderly people, is characterized by the formation of deposits beneath the retinal pigment epithelium (RPE) and by dysfunction of RPE and photoreceptor cells. The biologically active form of vitamin D, 1,25-(OH)2D3 (VITD), is...
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doaj-807657c2488646eda952c0ba823376482020-11-25T01:12:21ZengMDPI AGAntioxidants2076-39212019-08-018934110.3390/antiox8090341antiox8090341Vitamin D Attenuates Oxidative Damage and Inflammation in Retinal Pigment Epithelial CellsAli Mohammad Tohari0Reem Hasaballah Alhasani1Lincoln Biswas2Sarita Rani Patnaik3James Reilly4Zhihong Zeng5Xinhua Shu6Department of Clinical Biochemistry, King Fahad Hospital, PO Box 204, Jazan 91991, Saudi ArabiaDepartment of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow G4 0BA, UKDepartment of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow G4 0BA, UKDepartment of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow G4 0BA, UKDepartment of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow G4 0BA, UKDepartment of Bioengineering and Environmental Science, Changsha University, Changsha 410022, ChinaDepartment of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow G4 0BA, UKAge-related macular degeneration (AMD), the most common visual disorder in elderly people, is characterized by the formation of deposits beneath the retinal pigment epithelium (RPE) and by dysfunction of RPE and photoreceptor cells. The biologically active form of vitamin D, 1,25-(OH)2D3 (VITD), is categorized as a multifunctional steroid hormone that modulates many transcriptional processes of different genes and is involved in a broad range of cellular functions. Epidemiological and genetic association studies demonstrate that VITD may have a protective role in AMD, while single nucleotide polymorphisms in the vitamin D metabolism gene (<i>CYP24A1</i>) increase the risk of AMD. However, the functional mechanisms of VITD in AMD are not fully understood. In the current study, we investigated the impact of VITD on H<sub>2</sub>O<sub>2</sub>-induced oxidative stress and inflammation in human RPE cells. We demonstrate that exposure to H<sub>2</sub>O<sub>2</sub> caused significantly reduced cell viability, increased production of reactive oxygen species (ROS), lowered expression of antioxidant enzymes and enhanced inflammation. VITD exposure notably counteracted the above H<sub>2</sub>O<sub>2</sub>-induced effects. Our data suggest that VITD protects the RPE from oxidative damage and elucidate molecular mechanisms of VITD deficiency in the development of AMD.https://www.mdpi.com/2076-3921/8/9/341vitamin Doxidative stressinflammationretinal pigment epithelial cellsage-related macular degeneration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ali Mohammad Tohari Reem Hasaballah Alhasani Lincoln Biswas Sarita Rani Patnaik James Reilly Zhihong Zeng Xinhua Shu |
spellingShingle |
Ali Mohammad Tohari Reem Hasaballah Alhasani Lincoln Biswas Sarita Rani Patnaik James Reilly Zhihong Zeng Xinhua Shu Vitamin D Attenuates Oxidative Damage and Inflammation in Retinal Pigment Epithelial Cells Antioxidants vitamin D oxidative stress inflammation retinal pigment epithelial cells age-related macular degeneration |
author_facet |
Ali Mohammad Tohari Reem Hasaballah Alhasani Lincoln Biswas Sarita Rani Patnaik James Reilly Zhihong Zeng Xinhua Shu |
author_sort |
Ali Mohammad Tohari |
title |
Vitamin D Attenuates Oxidative Damage and Inflammation in Retinal Pigment Epithelial Cells |
title_short |
Vitamin D Attenuates Oxidative Damage and Inflammation in Retinal Pigment Epithelial Cells |
title_full |
Vitamin D Attenuates Oxidative Damage and Inflammation in Retinal Pigment Epithelial Cells |
title_fullStr |
Vitamin D Attenuates Oxidative Damage and Inflammation in Retinal Pigment Epithelial Cells |
title_full_unstemmed |
Vitamin D Attenuates Oxidative Damage and Inflammation in Retinal Pigment Epithelial Cells |
title_sort |
vitamin d attenuates oxidative damage and inflammation in retinal pigment epithelial cells |
publisher |
MDPI AG |
series |
Antioxidants |
issn |
2076-3921 |
publishDate |
2019-08-01 |
description |
Age-related macular degeneration (AMD), the most common visual disorder in elderly people, is characterized by the formation of deposits beneath the retinal pigment epithelium (RPE) and by dysfunction of RPE and photoreceptor cells. The biologically active form of vitamin D, 1,25-(OH)2D3 (VITD), is categorized as a multifunctional steroid hormone that modulates many transcriptional processes of different genes and is involved in a broad range of cellular functions. Epidemiological and genetic association studies demonstrate that VITD may have a protective role in AMD, while single nucleotide polymorphisms in the vitamin D metabolism gene (<i>CYP24A1</i>) increase the risk of AMD. However, the functional mechanisms of VITD in AMD are not fully understood. In the current study, we investigated the impact of VITD on H<sub>2</sub>O<sub>2</sub>-induced oxidative stress and inflammation in human RPE cells. We demonstrate that exposure to H<sub>2</sub>O<sub>2</sub> caused significantly reduced cell viability, increased production of reactive oxygen species (ROS), lowered expression of antioxidant enzymes and enhanced inflammation. VITD exposure notably counteracted the above H<sub>2</sub>O<sub>2</sub>-induced effects. Our data suggest that VITD protects the RPE from oxidative damage and elucidate molecular mechanisms of VITD deficiency in the development of AMD. |
topic |
vitamin D oxidative stress inflammation retinal pigment epithelial cells age-related macular degeneration |
url |
https://www.mdpi.com/2076-3921/8/9/341 |
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