ISOLATION AND CULTURE OF MESENCHYMAL STEM CELLS DERIVED FROM ADIPOSE TISSUE AND BONE MARROW OF DOGS
The objective of this study was to establish a protocol for the isolation and culture of mesenchymal stem cells (MSC) from bone marrow and adipose tissue of dogs. Three 6-month-old male dogs were used. Approximately 3.0 cm3 of adipose tissue and 3.0 mL of bone marrow were collected. The samples were...
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Universidade Federal de Goiás
2017-07-01
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doaj-808094f01332456e8eae3b267b251ccc2020-11-25T03:57:10ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971809-68912017-07-0118018489ISOLATION AND CULTURE OF MESENCHYMAL STEM CELLS DERIVED FROM ADIPOSE TISSUE AND BONE MARROW OF DOGSEndrigo Gabellini Leonel Alves0Rogéria Serakides1Isabel Rodrigues Rosado2Jankerle Neves Boeloni3Natalia Melo Ocarino4Cleuza Maria de Faria Rezende5Universidade de Uberaba (UNIUBE)Escola de Veterinária da UFMG, DocenteUniversidade de Uberaba (UNIUBE), DocenteDepartamento de Medicina Veterinária do CCA/UFES, DocenteEscola de Veterinária da UFMG, DocenteEscola de Veterinária da UFMG, DocenteThe objective of this study was to establish a protocol for the isolation and culture of mesenchymal stem cells (MSC) from bone marrow and adipose tissue of dogs. Three 6-month-old male dogs were used. Approximately 3.0 cm3 of adipose tissue and 3.0 mL of bone marrow were collected. The samples were processed and the isolated cells were cultured in DMEM. The cells were subjected to phenotypic characterization and to osteogenic, adipogenic, and condrogenic differentiation to confirm the isolation of the MSC. The cells showed elongated and fusiform morphology and they were able to differentiate into osteoblasts, adipocytes, and chondrocytes. Phenotypic characterization revealed high expression of the MSC markers CD90(80.04%) and CD29(96%) in the cells from bone marrow and high expression of CD90(60.94%) and CD29(77.08%) in the cells from adipose tissue. In addition, phenotypic characterization revealed low expression of hematopoietic markers CD45(1.45%) and CD34(1.53%) in the cells from bone marrow and low expression of CD45(1.45%) and CD34(1.53%) in the cells from adipose tissue. Based on these results, the modifications applied to classical protocols simplified the process and proved to be efficient in the isolation, culture, and expansion of MSC isolated from the bone marrow and adipose tissue of dogs. Keywords: cell culture; differentiation; regenerative medicine.https://www.revistas.ufg.br/vet/article/view/34050medicina regenerativacultivo celulardiferenciação |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Endrigo Gabellini Leonel Alves Rogéria Serakides Isabel Rodrigues Rosado Jankerle Neves Boeloni Natalia Melo Ocarino Cleuza Maria de Faria Rezende |
spellingShingle |
Endrigo Gabellini Leonel Alves Rogéria Serakides Isabel Rodrigues Rosado Jankerle Neves Boeloni Natalia Melo Ocarino Cleuza Maria de Faria Rezende ISOLATION AND CULTURE OF MESENCHYMAL STEM CELLS DERIVED FROM ADIPOSE TISSUE AND BONE MARROW OF DOGS Ciência Animal Brasileira medicina regenerativa cultivo celular diferenciação |
author_facet |
Endrigo Gabellini Leonel Alves Rogéria Serakides Isabel Rodrigues Rosado Jankerle Neves Boeloni Natalia Melo Ocarino Cleuza Maria de Faria Rezende |
author_sort |
Endrigo Gabellini Leonel Alves |
title |
ISOLATION AND CULTURE OF MESENCHYMAL STEM CELLS DERIVED FROM ADIPOSE TISSUE AND BONE MARROW OF DOGS |
title_short |
ISOLATION AND CULTURE OF MESENCHYMAL STEM CELLS DERIVED FROM ADIPOSE TISSUE AND BONE MARROW OF DOGS |
title_full |
ISOLATION AND CULTURE OF MESENCHYMAL STEM CELLS DERIVED FROM ADIPOSE TISSUE AND BONE MARROW OF DOGS |
title_fullStr |
ISOLATION AND CULTURE OF MESENCHYMAL STEM CELLS DERIVED FROM ADIPOSE TISSUE AND BONE MARROW OF DOGS |
title_full_unstemmed |
ISOLATION AND CULTURE OF MESENCHYMAL STEM CELLS DERIVED FROM ADIPOSE TISSUE AND BONE MARROW OF DOGS |
title_sort |
isolation and culture of mesenchymal stem cells derived from adipose tissue and bone marrow of dogs |
publisher |
Universidade Federal de Goiás |
series |
Ciência Animal Brasileira |
issn |
1518-2797 1809-6891 |
publishDate |
2017-07-01 |
description |
The objective of this study was to establish a protocol for the isolation and culture of mesenchymal stem cells (MSC) from bone marrow and adipose tissue of dogs. Three 6-month-old male dogs were used. Approximately 3.0 cm3 of adipose tissue and 3.0 mL of bone marrow were collected. The samples were processed and the isolated cells were cultured in DMEM. The cells were subjected to phenotypic characterization and to osteogenic, adipogenic, and condrogenic differentiation to confirm the isolation of the MSC. The cells showed elongated and fusiform morphology and they were able to differentiate into osteoblasts, adipocytes, and chondrocytes. Phenotypic characterization revealed high expression of the MSC markers CD90(80.04%) and CD29(96%) in the cells from bone marrow and high expression of CD90(60.94%) and CD29(77.08%) in the cells from adipose tissue. In addition, phenotypic characterization revealed low expression of hematopoietic markers CD45(1.45%) and CD34(1.53%) in the cells from bone marrow and low expression of CD45(1.45%) and CD34(1.53%) in the cells from adipose tissue. Based on these results, the modifications applied to classical protocols simplified the process and proved to be efficient in the isolation, culture, and expansion of MSC isolated from the bone marrow and adipose tissue of dogs.
Keywords: cell culture; differentiation; regenerative medicine. |
topic |
medicina regenerativa cultivo celular diferenciação |
url |
https://www.revistas.ufg.br/vet/article/view/34050 |
work_keys_str_mv |
AT endrigogabellinileonelalves isolationandcultureofmesenchymalstemcellsderivedfromadiposetissueandbonemarrowofdogs AT rogeriaserakides isolationandcultureofmesenchymalstemcellsderivedfromadiposetissueandbonemarrowofdogs AT isabelrodriguesrosado isolationandcultureofmesenchymalstemcellsderivedfromadiposetissueandbonemarrowofdogs AT jankerlenevesboeloni isolationandcultureofmesenchymalstemcellsderivedfromadiposetissueandbonemarrowofdogs AT nataliameloocarino isolationandcultureofmesenchymalstemcellsderivedfromadiposetissueandbonemarrowofdogs AT cleuzamariadefariarezende isolationandcultureofmesenchymalstemcellsderivedfromadiposetissueandbonemarrowofdogs |
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