Label-Free Biosensor Using a Silver Specific RNA-Cleaving DNAzyme Functionalized Single-Walled Carbon Nanotube for Silver Ion Determination
Silver, a very common heavy metal, has been employed in electronics, medicine, jewelry, and catalysis due to its excellent chemical and physical characteristics. Silver-containing wastes can cause environmental pollution, so it is vital to monitor the Ag(I) concentration. Here, a label-free biosenso...
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doaj-8093e96d427b44aa83649db5e96778072020-11-24T22:58:21ZengMDPI AGNanomaterials2079-49912018-04-018425810.3390/nano8040258nano8040258Label-Free Biosensor Using a Silver Specific RNA-Cleaving DNAzyme Functionalized Single-Walled Carbon Nanotube for Silver Ion DeterminationHui Wang0Yang Liu1Gang Liu2Key Laboratory of Modern Precision Agriculture System Integration Research, Ministry of Education and Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture China Agricultural University, Beijing 100083, ChinaSchool of Electronic and Information Engineering, Xi’an Jiaotong University, Xi’an 710049, ChinaKey Laboratory of Modern Precision Agriculture System Integration Research, Ministry of Education and Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture China Agricultural University, Beijing 100083, ChinaSilver, a very common heavy metal, has been employed in electronics, medicine, jewelry, and catalysis due to its excellent chemical and physical characteristics. Silver-containing wastes can cause environmental pollution, so it is vital to monitor the Ag(I) concentration. Here, a label-free biosensor was developed for the Ag(I) detection, which used single-walled carbon nanotubes/field effect transistor (SWNTs/FET) to functionalize with a specific DNAzyme, containing an Agzyme and a complementary strand DNA (CS-DNA) embedded an RNA-base. The CS-DNA was covalently immobilized on the SWNTs’ surface through peptide bonds, and then combined with the Agzyme. When Ag(I) was bound with the Agzyme, the CS-DNA can be cleaved at the RNA site efficiently. The cleaved DNAzyme induced a remarkable change in the electrical conductivity of SWNTs. The performances of DNAzyme/SWNTs/FET were investigated using different spectroscopy and electrochemical methods. Under the optimized parameters, DNAzyme/SWNTs/FET presented a high sensitivity and selectivity towards Ag(I), in which the linear response range is 10 pM to 106 pM and the limit of detection is 5 pM(S/N = 3). Additionally, the prepared biosensor was applied to measure the Ag(I) concentration in the water sample with good results.http://www.mdpi.com/2079-4991/8/4/258field effect transistorsingle-walled carbon nanotubessilver ionbiosensorDNAzyme |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hui Wang Yang Liu Gang Liu |
spellingShingle |
Hui Wang Yang Liu Gang Liu Label-Free Biosensor Using a Silver Specific RNA-Cleaving DNAzyme Functionalized Single-Walled Carbon Nanotube for Silver Ion Determination Nanomaterials field effect transistor single-walled carbon nanotubes silver ion biosensor DNAzyme |
author_facet |
Hui Wang Yang Liu Gang Liu |
author_sort |
Hui Wang |
title |
Label-Free Biosensor Using a Silver Specific RNA-Cleaving DNAzyme Functionalized Single-Walled Carbon Nanotube for Silver Ion Determination |
title_short |
Label-Free Biosensor Using a Silver Specific RNA-Cleaving DNAzyme Functionalized Single-Walled Carbon Nanotube for Silver Ion Determination |
title_full |
Label-Free Biosensor Using a Silver Specific RNA-Cleaving DNAzyme Functionalized Single-Walled Carbon Nanotube for Silver Ion Determination |
title_fullStr |
Label-Free Biosensor Using a Silver Specific RNA-Cleaving DNAzyme Functionalized Single-Walled Carbon Nanotube for Silver Ion Determination |
title_full_unstemmed |
Label-Free Biosensor Using a Silver Specific RNA-Cleaving DNAzyme Functionalized Single-Walled Carbon Nanotube for Silver Ion Determination |
title_sort |
label-free biosensor using a silver specific rna-cleaving dnazyme functionalized single-walled carbon nanotube for silver ion determination |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2018-04-01 |
description |
Silver, a very common heavy metal, has been employed in electronics, medicine, jewelry, and catalysis due to its excellent chemical and physical characteristics. Silver-containing wastes can cause environmental pollution, so it is vital to monitor the Ag(I) concentration. Here, a label-free biosensor was developed for the Ag(I) detection, which used single-walled carbon nanotubes/field effect transistor (SWNTs/FET) to functionalize with a specific DNAzyme, containing an Agzyme and a complementary strand DNA (CS-DNA) embedded an RNA-base. The CS-DNA was covalently immobilized on the SWNTs’ surface through peptide bonds, and then combined with the Agzyme. When Ag(I) was bound with the Agzyme, the CS-DNA can be cleaved at the RNA site efficiently. The cleaved DNAzyme induced a remarkable change in the electrical conductivity of SWNTs. The performances of DNAzyme/SWNTs/FET were investigated using different spectroscopy and electrochemical methods. Under the optimized parameters, DNAzyme/SWNTs/FET presented a high sensitivity and selectivity towards Ag(I), in which the linear response range is 10 pM to 106 pM and the limit of detection is 5 pM(S/N = 3). Additionally, the prepared biosensor was applied to measure the Ag(I) concentration in the water sample with good results. |
topic |
field effect transistor single-walled carbon nanotubes silver ion biosensor DNAzyme |
url |
http://www.mdpi.com/2079-4991/8/4/258 |
work_keys_str_mv |
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