Algorithms for testing COVID-19 focused on use of RT-PCR and high-affinity serological testing: A consensus statement from a panel of Latin American experts
The COVID-19 pandemic has caused an unprecedented public health, social, and economic crisis. Improving understanding on available tests for detecting COVID-19 is critical for effective management of the pandemic. We proposed that a multidisciplinary expert panel can establish recommendations on ide...
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doaj-809868d920684a5abb730442954cdb862021-02-07T04:21:42ZengElsevierInternational Journal of Infectious Diseases1201-97122021-02-01103260267Algorithms for testing COVID-19 focused on use of RT-PCR and high-affinity serological testing: A consensus statement from a panel of Latin American expertsCarlos Eduardo Ferreira0Pablo E. Bonvehi1Juan Carlos Gómez de la Torre2Klever Vinicio Sáenz-Flor3Antonio Condino-Neto4Hospital Israelita Albert Einstein, São Paulo, Brazil; President of the Brazilian Society of Clinical Pathology/Laboratory Medicine; Corresponding author at: Albert Einstein Hospital, Av. Albert Einstein, 627, Second Floor, Block E, Clinical Laboratory, Morumbi, São Paulo, SP, 05652-000, Brazil.Infectious Diseases Unit, Clinical Investigation and Medical Education Center (CEMIC), Buenos Aires, ArgentinaMedical Science Faculty, Ricardo Palma l University, Lima, Peru; Peruvian Laboratory of Molecular and Genetic Medicine, PeruMedical Sciences Faculty, Central University of Equator, Quito, Ecuador; Member of the World Association of Societies of Pathology & Laboratory MedicineInstitute of Biomedical Sciences, University of São Paulo, Brazil; Director of the Jeffrey Modell Center for Immunodeficiencies, São Paulo, BrazilThe COVID-19 pandemic has caused an unprecedented public health, social, and economic crisis. Improving understanding on available tests for detecting COVID-19 is critical for effective management of the pandemic. We proposed that a multidisciplinary expert panel can establish recommendations on ideal use of diagnostic tools, with a focus on RT-PCR and serological high-affinity antibodies (both IgM and IgG) tests for the Latin America region. Study design: A collaborative multidisciplinary panel of 5 recognized experts in Latin America (an infectious disease specialist, three pathologists, and an immunologist) was convened and supported by Roche Diagnostics to develop standard guidelines and an evidence-based document of best practices on the use of diagnostic tools for COVID-19. Results: The authors reached consensus on the applicability of diagnostic tools to provide testing algorithms for the use of RT-PCR and serological high-affinity antibodies (both IgM and IgG) tests in three settings: 1) For asymptomatic subjects exposed to a SARS-CoV-2 infected person; 2) For epidemiological purposes and; 3) For symptomatic subjects. Conclusion: The serological high-affinity SARS-CoV-2 antibodies (both IgM and IgG) tests play a key role in COVID-19 diagnosis. These tests can be applied for suspected false-negative RT-PCR results and for individual determination of response. The use of these tests can also contribute greatly to public health strategies, such as population screening and supporting vaccination planning. Serological status for high-affinity antibodies (both IgM and IgG) should be performed ideally 21 days after potential infectious contact, given that the majority of exposed individuals will have seroconverted.http://www.sciencedirect.com/science/article/pii/S120197122032484XCOVID-19DiagnosisSerological testHigh-affinity antibodiesRT-PCR |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carlos Eduardo Ferreira Pablo E. Bonvehi Juan Carlos Gómez de la Torre Klever Vinicio Sáenz-Flor Antonio Condino-Neto |
spellingShingle |
Carlos Eduardo Ferreira Pablo E. Bonvehi Juan Carlos Gómez de la Torre Klever Vinicio Sáenz-Flor Antonio Condino-Neto Algorithms for testing COVID-19 focused on use of RT-PCR and high-affinity serological testing: A consensus statement from a panel of Latin American experts International Journal of Infectious Diseases COVID-19 Diagnosis Serological test High-affinity antibodies RT-PCR |
author_facet |
Carlos Eduardo Ferreira Pablo E. Bonvehi Juan Carlos Gómez de la Torre Klever Vinicio Sáenz-Flor Antonio Condino-Neto |
author_sort |
Carlos Eduardo Ferreira |
title |
Algorithms for testing COVID-19 focused on use of RT-PCR and high-affinity serological testing: A consensus statement from a panel of Latin American experts |
title_short |
Algorithms for testing COVID-19 focused on use of RT-PCR and high-affinity serological testing: A consensus statement from a panel of Latin American experts |
title_full |
Algorithms for testing COVID-19 focused on use of RT-PCR and high-affinity serological testing: A consensus statement from a panel of Latin American experts |
title_fullStr |
Algorithms for testing COVID-19 focused on use of RT-PCR and high-affinity serological testing: A consensus statement from a panel of Latin American experts |
title_full_unstemmed |
Algorithms for testing COVID-19 focused on use of RT-PCR and high-affinity serological testing: A consensus statement from a panel of Latin American experts |
title_sort |
algorithms for testing covid-19 focused on use of rt-pcr and high-affinity serological testing: a consensus statement from a panel of latin american experts |
publisher |
Elsevier |
series |
International Journal of Infectious Diseases |
issn |
1201-9712 |
publishDate |
2021-02-01 |
description |
The COVID-19 pandemic has caused an unprecedented public health, social, and economic crisis. Improving understanding on available tests for detecting COVID-19 is critical for effective management of the pandemic. We proposed that a multidisciplinary expert panel can establish recommendations on ideal use of diagnostic tools, with a focus on RT-PCR and serological high-affinity antibodies (both IgM and IgG) tests for the Latin America region. Study design: A collaborative multidisciplinary panel of 5 recognized experts in Latin America (an infectious disease specialist, three pathologists, and an immunologist) was convened and supported by Roche Diagnostics to develop standard guidelines and an evidence-based document of best practices on the use of diagnostic tools for COVID-19. Results: The authors reached consensus on the applicability of diagnostic tools to provide testing algorithms for the use of RT-PCR and serological high-affinity antibodies (both IgM and IgG) tests in three settings: 1) For asymptomatic subjects exposed to a SARS-CoV-2 infected person; 2) For epidemiological purposes and; 3) For symptomatic subjects. Conclusion: The serological high-affinity SARS-CoV-2 antibodies (both IgM and IgG) tests play a key role in COVID-19 diagnosis. These tests can be applied for suspected false-negative RT-PCR results and for individual determination of response. The use of these tests can also contribute greatly to public health strategies, such as population screening and supporting vaccination planning. Serological status for high-affinity antibodies (both IgM and IgG) should be performed ideally 21 days after potential infectious contact, given that the majority of exposed individuals will have seroconverted. |
topic |
COVID-19 Diagnosis Serological test High-affinity antibodies RT-PCR |
url |
http://www.sciencedirect.com/science/article/pii/S120197122032484X |
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