Providing Flaxseed Oil but Not Menhaden Oil Protects against OVX Induced Bone Loss in the Mandible of Sprague-Dawley Rats
Higher intakes of polyunsaturated fatty acids (PUFA) are associated with benefits at several skeletal sites in postmenopausal women and in rodent models, but the effect of PUFA-containing oils on tooth-supporting alveolar bone of the mandible has not been studied. Moreover, direct comparison of the...
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doaj-80a3bf74c99244b2bbeb8fdc4beba6ce2020-11-24T20:49:58ZengMDPI AGNutrients2072-66432016-09-0181059710.3390/nu8100597nu8100597Providing Flaxseed Oil but Not Menhaden Oil Protects against OVX Induced Bone Loss in the Mandible of Sprague-Dawley RatsAmanda B. Longo0Wendy E. Ward1Faculty of Applied Health Sciences, Brock University, St. Catharines, ON L2S 3A1, CanadaFaculty of Applied Health Sciences, Brock University, St. Catharines, ON L2S 3A1, CanadaHigher intakes of polyunsaturated fatty acids (PUFA) are associated with benefits at several skeletal sites in postmenopausal women and in rodent models, but the effect of PUFA-containing oils on tooth-supporting alveolar bone of the mandible has not been studied. Moreover, direct comparison of the effect of flaxseed oil (a source of alpha-linolenic acid (ALA)) and menhaden oil (a source of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) is unknown. One-month old female Sprague-Dawley rats (n = 48) were randomized to and fed a diet containing flaxseed oil or menhaden oil from one to six months of age. At three months of age, rats were randomized to receive SHAM or ovariectomy (OVX) surgery (n = 12/diet). The inter-radicular septum below the first molar of the mandible was imaged at 6 months of age (study endpoint) using micro-computed tomography (μCT) at a resolution of 9 μm. As expected, OVX significantly reduced percent bone volume (BV/TV), connectivity density (Conn. D.), trabecular number (Tb. N.), and increased trabecular separation (Tb. Sp.) compared to SHAM rats (p < 0.001). However, post hoc analysis revealed these differences were present in rats fed menhaden oil but not those fed flaxseed oil. These results suggest that providing flaxseed oil, possibly through its high ALA content, provides protection against the OVX-induced alveolar bone loss in rats.http://www.mdpi.com/2072-6643/8/10/597alveolar bonemandiblemicro-computed tomographyomega-3 fatty aidsalpha-linolenic acideicosapentaenoic aciddocosahexaenoic acidovariectomyflaxseed oilfish oil |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Amanda B. Longo Wendy E. Ward |
spellingShingle |
Amanda B. Longo Wendy E. Ward Providing Flaxseed Oil but Not Menhaden Oil Protects against OVX Induced Bone Loss in the Mandible of Sprague-Dawley Rats Nutrients alveolar bone mandible micro-computed tomography omega-3 fatty aids alpha-linolenic acid eicosapentaenoic acid docosahexaenoic acid ovariectomy flaxseed oil fish oil |
author_facet |
Amanda B. Longo Wendy E. Ward |
author_sort |
Amanda B. Longo |
title |
Providing Flaxseed Oil but Not Menhaden Oil Protects against OVX Induced Bone Loss in the Mandible of Sprague-Dawley Rats |
title_short |
Providing Flaxseed Oil but Not Menhaden Oil Protects against OVX Induced Bone Loss in the Mandible of Sprague-Dawley Rats |
title_full |
Providing Flaxseed Oil but Not Menhaden Oil Protects against OVX Induced Bone Loss in the Mandible of Sprague-Dawley Rats |
title_fullStr |
Providing Flaxseed Oil but Not Menhaden Oil Protects against OVX Induced Bone Loss in the Mandible of Sprague-Dawley Rats |
title_full_unstemmed |
Providing Flaxseed Oil but Not Menhaden Oil Protects against OVX Induced Bone Loss in the Mandible of Sprague-Dawley Rats |
title_sort |
providing flaxseed oil but not menhaden oil protects against ovx induced bone loss in the mandible of sprague-dawley rats |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2016-09-01 |
description |
Higher intakes of polyunsaturated fatty acids (PUFA) are associated with benefits at several skeletal sites in postmenopausal women and in rodent models, but the effect of PUFA-containing oils on tooth-supporting alveolar bone of the mandible has not been studied. Moreover, direct comparison of the effect of flaxseed oil (a source of alpha-linolenic acid (ALA)) and menhaden oil (a source of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) is unknown. One-month old female Sprague-Dawley rats (n = 48) were randomized to and fed a diet containing flaxseed oil or menhaden oil from one to six months of age. At three months of age, rats were randomized to receive SHAM or ovariectomy (OVX) surgery (n = 12/diet). The inter-radicular septum below the first molar of the mandible was imaged at 6 months of age (study endpoint) using micro-computed tomography (μCT) at a resolution of 9 μm. As expected, OVX significantly reduced percent bone volume (BV/TV), connectivity density (Conn. D.), trabecular number (Tb. N.), and increased trabecular separation (Tb. Sp.) compared to SHAM rats (p < 0.001). However, post hoc analysis revealed these differences were present in rats fed menhaden oil but not those fed flaxseed oil. These results suggest that providing flaxseed oil, possibly through its high ALA content, provides protection against the OVX-induced alveolar bone loss in rats. |
topic |
alveolar bone mandible micro-computed tomography omega-3 fatty aids alpha-linolenic acid eicosapentaenoic acid docosahexaenoic acid ovariectomy flaxseed oil fish oil |
url |
http://www.mdpi.com/2072-6643/8/10/597 |
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