In vitro antimicrobial activity of the organic extract of Cladonia substellata Vainio and usnic acid against Staphylococcus spp. obtained from cats and dogs

• Main Authors: Jusciêne B. Moura, Agueda C. de Vargas, Gisele V. Gouveia, João J. de S. Gouveia, Juracy C. Ramos-Júnior, Sônia de A. Botton, Eugênia C. Pereira, Mateus M. da Costa
• Format: Article
• Language: English
• Published: Colégio Brasileiro de Patologia Animal (CBPA)
• Series: Pesquisa Veterinária Brasileira
• Subjects: Antimicrobial activity; Cladonia substellata; lichens; usnic acid; Staphylococcus spp.; cats; dogs; bacteria; biofilm; genes.
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2017000400368&lng=en&tlng=en

ABSTRACT: Cladonia substellata Vainio is a lichen found in different regions of the world, including the Northeast of Brazil. It contains several secondary metabolites with biological activity, including usnic acid, which has exhibited a wide range of biological activities. The aim of this study was to evaluate the in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid. Initially, Staphylococcus spp., derived from samples of skin and ears of dogs and cats with suspected pyoderma and otitis, were isolated and analyzed. In antimicrobial susceptibility testing against Staphylococcus spp., 77% (105/136) of the isolates were resistant to the antimicrobials tested. In the assessment of biofilm production, 83% (113/136) were classified as producing biofilm. In genetic characterization, 32% (44/136) were positive for blaZ, no isolate (0/136) was positive for the mecA gene, and 2% (3/136) were positive for the icaD gene. The in vitro antimicrobial activity of the organic extract of C. substellata and purified usnic acid against Staphylococcus spp. ranged from 0.25mg/mL to 0.0019mg/mL, inhibiting bacterial growth at low concentrations. The substances were more effective against biofilm-producing bacteria (0.65mg/mL-0.42mg/mL) when compared to non-biofilm producing bacteria (2.52mg/mL-2.71mg/mL). Usnic acid and the organic extract of C. substellata can be effective in the treatment of pyoderma and otitis in dogs and cats caused by Staphylococcus spp.