Aging increases Oxidative Stress and Renal Expression of Oxidant and Antioxidant Enzymes that Are Associated with an Increased Trend in Systolic Blood Pressure
The aim of this study was to investigate whether the effects of aging on oxidative stress markers and expression of major oxidant and antioxidant enzymes associate with impairment of renal function and increases in blood pressure. To explore this, we determined age-associated changes in lipid peroxi...
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Online Access: | http://dx.doi.org/10.4161/oxim.2.3.8819 |
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doaj-80c5d803b207424d81e09647829097862020-11-24T23:14:53ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09001942-09942009-01-012313814510.4161/oxim.2.3.8819Aging increases Oxidative Stress and Renal Expression of Oxidant and Antioxidant Enzymes that Are Associated with an Increased Trend in Systolic Blood PressurePedro Gomes0Sónia Simão1Elisabete Silva2Vanda Pinto3João S. Amaral4Joana Afonso5Maria Paula Serrão6Maria João Pinho7Patrício Soares-da-Silva8Institute of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, PortugalInstitute of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, PortugalInstitute of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, PortugalInstitute of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, PortugalInstitute of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, PortugalInstitute of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, PortugalInstitute of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, PortugalInstitute of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, PortugalInstitute of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, Porto, PortugalThe aim of this study was to investigate whether the effects of aging on oxidative stress markers and expression of major oxidant and antioxidant enzymes associate with impairment of renal function and increases in blood pressure. To explore this, we determined age-associated changes in lipid peroxidation (urinary malondialdehyde), plasma and urinary hydrogen peroxide (H2O2) levels, as well as renal H2O2 production, and the expression of oxidant and antioxidant enzymes in young (13 weeks) and old (52 weeks) male Wistar Kyoto (WKY) rats. Urinary lipid peroxidation levels and H2O2 production by the renal cortex and medulla of old rats were higher than their young counterparts. This was accompanied by overexpression of NADPH oxidase components Nox4 and p22phox in the renal cortex of old rats. Similarly, expression of superoxide dismutase (SOD) isoforms 2 and 3 and catalase were increased in the renal cortex from old rats. Renal function parameters (creatinine clearance and fractional excretion of sodium), diastolic blood pressure and heart rate were not affected by aging, although slight increases in systolic blood pressure were observed during this 52-week period. It is concluded that overexpression of renal Nox4 and p22phox and the increases in renal H2O2 levels in aged WKY does not associate with renal functional impairment or marked increases in blood pressure. It is hypothesized that lack of oxidative stress-associated effects in aged WKY rats may result from increases in antioxidant defenses that counteract the damaging effects of H2O2.http://dx.doi.org/10.4161/oxim.2.3.8819 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pedro Gomes Sónia Simão Elisabete Silva Vanda Pinto João S. Amaral Joana Afonso Maria Paula Serrão Maria João Pinho Patrício Soares-da-Silva |
spellingShingle |
Pedro Gomes Sónia Simão Elisabete Silva Vanda Pinto João S. Amaral Joana Afonso Maria Paula Serrão Maria João Pinho Patrício Soares-da-Silva Aging increases Oxidative Stress and Renal Expression of Oxidant and Antioxidant Enzymes that Are Associated with an Increased Trend in Systolic Blood Pressure Oxidative Medicine and Cellular Longevity |
author_facet |
Pedro Gomes Sónia Simão Elisabete Silva Vanda Pinto João S. Amaral Joana Afonso Maria Paula Serrão Maria João Pinho Patrício Soares-da-Silva |
author_sort |
Pedro Gomes |
title |
Aging increases Oxidative Stress and Renal Expression of Oxidant and Antioxidant Enzymes that Are Associated with an Increased Trend in Systolic Blood Pressure |
title_short |
Aging increases Oxidative Stress and Renal Expression of Oxidant and Antioxidant Enzymes that Are Associated with an Increased Trend in Systolic Blood Pressure |
title_full |
Aging increases Oxidative Stress and Renal Expression of Oxidant and Antioxidant Enzymes that Are Associated with an Increased Trend in Systolic Blood Pressure |
title_fullStr |
Aging increases Oxidative Stress and Renal Expression of Oxidant and Antioxidant Enzymes that Are Associated with an Increased Trend in Systolic Blood Pressure |
title_full_unstemmed |
Aging increases Oxidative Stress and Renal Expression of Oxidant and Antioxidant Enzymes that Are Associated with an Increased Trend in Systolic Blood Pressure |
title_sort |
aging increases oxidative stress and renal expression of oxidant and antioxidant enzymes that are associated with an increased trend in systolic blood pressure |
publisher |
Hindawi Limited |
series |
Oxidative Medicine and Cellular Longevity |
issn |
1942-0900 1942-0994 |
publishDate |
2009-01-01 |
description |
The aim of this study was to investigate whether the effects of aging on oxidative stress markers and expression of major oxidant and antioxidant enzymes associate with impairment of renal function and increases in blood pressure. To explore this, we determined age-associated changes in lipid peroxidation (urinary malondialdehyde), plasma and urinary hydrogen peroxide (H2O2) levels, as well as renal H2O2 production, and the expression of oxidant and antioxidant enzymes in young (13 weeks) and old (52 weeks) male Wistar Kyoto (WKY) rats. Urinary lipid peroxidation levels and H2O2 production by the renal cortex and medulla of old rats were higher than their young counterparts. This was accompanied by overexpression of NADPH oxidase components Nox4 and p22phox in the renal cortex of old rats. Similarly, expression of superoxide dismutase (SOD) isoforms 2 and 3 and catalase were increased in the renal cortex from old rats. Renal function parameters (creatinine clearance and fractional excretion of sodium), diastolic blood pressure and heart rate were not affected by aging, although slight increases in systolic blood pressure were observed during this 52-week period. It is concluded that overexpression of renal Nox4 and p22phox and the increases in renal H2O2 levels in aged WKY does not associate with renal functional impairment or marked increases in blood pressure. It is hypothesized that lack of oxidative stress-associated effects in aged WKY rats may result from increases in antioxidant defenses that counteract the damaging effects of H2O2. |
url |
http://dx.doi.org/10.4161/oxim.2.3.8819 |
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