The role of microbes in rumen lipolysis and biohydrogenation and their manipulation
Despite the fact that the ruminant diet is rich in polyunsaturated fatty acids (PUFA), ruminant products – meat, milk and dairy – contain mainly saturated fatty acids (SFA) because of bacterial lipolysis and subsequent biohydrogenation of ingested PUFA in the rumen. The link between SFA consumption...
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doaj-80d0c957482b439aa450e2d7b737eefe2021-06-05T06:06:53ZengElsevierAnimal1751-73112010-01-014710081023The role of microbes in rumen lipolysis and biohydrogenation and their manipulationM. Lourenço0E. Ramos-Morales1R.J. Wallace2Department of Animal Production, Ghent University, Laboratory for Animal Nutrition and Animal Product Quality, Proefhoevestraat 10, 9090 Melle, BelgiumGut Health Division, University of Aberdeen, Rowett Institute of Nutrition and Health, Greenburn Road, Bucksburn, UKGut Health Division, University of Aberdeen, Rowett Institute of Nutrition and Health, Greenburn Road, Bucksburn, UKDespite the fact that the ruminant diet is rich in polyunsaturated fatty acids (PUFA), ruminant products – meat, milk and dairy – contain mainly saturated fatty acids (SFA) because of bacterial lipolysis and subsequent biohydrogenation of ingested PUFA in the rumen. The link between SFA consumption by man and coronary heart disease is well established. In contrast, ruminant products also contain fatty acids that are known to be beneficial to human health, namely conjugated linoleic acids (CLAs). The aims of research in this field have been to understand the microbial ecology of lipolysis and biohydrogenation and to find ways of manipulating ruminal microbes to increase the flow of PUFA and CLA from the rumen into meat and milk. This review describes our present understanding of the microbial ecology of ruminal lipid metabolism, including some apparently anomalous and paradoxical observations, and the status of how the metabolism may be manipulated and the possible consequential effects on other aspects of ruminal digestion. Intuitively, it may appear that inhibiting the ruminal lipase would cause more dietary PUFA to reach the mammary gland. However, lipolysis releases the non-esterified fatty acids that form the substrates for biohydrogenation, but which can, if they accumulate, inhibit the whole process. Thus, increasing lipase activity could be beneficial if the increased release of non-esterified PUFA inhibited the metabolism of CLA. Rumen ciliate protozoa do not carry out biohydrogenation, yet protozoal lipids are much more highly enriched in CLA than bacterial lipids. How could this happen if protozoa do not metabolise PUFA? The answer seems to lie in the ingestion of plant organelles, particularly chloroplasts, and the partial metabolism of the fatty acids by contaminating bacteria. Bacteria related to Butyrivibrio fibrisolvens are by far the most active and numerous biohydrogenating bacteria isolated from the rumen. But do we misunderstand the role of different bacterial species in biohydrogenation because there are uncultivated species that we need to understand and include in the analysis? Manipulation methods include dietary vegetable and fish oils and plant-derived chemicals. Their usefulness, efficacy and possible effects on fatty acid metabolism and on ruminal microorganisms and other areas of their metabolism are described, and areas of opportunity identified.http://www.sciencedirect.com/science/article/pii/S175173111000042Xbiohydrogenationcellulose digestionlipasemicrobial protein synthesisrumen |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M. Lourenço E. Ramos-Morales R.J. Wallace |
spellingShingle |
M. Lourenço E. Ramos-Morales R.J. Wallace The role of microbes in rumen lipolysis and biohydrogenation and their manipulation Animal biohydrogenation cellulose digestion lipase microbial protein synthesis rumen |
author_facet |
M. Lourenço E. Ramos-Morales R.J. Wallace |
author_sort |
M. Lourenço |
title |
The role of microbes in rumen lipolysis and biohydrogenation and their manipulation |
title_short |
The role of microbes in rumen lipolysis and biohydrogenation and their manipulation |
title_full |
The role of microbes in rumen lipolysis and biohydrogenation and their manipulation |
title_fullStr |
The role of microbes in rumen lipolysis and biohydrogenation and their manipulation |
title_full_unstemmed |
The role of microbes in rumen lipolysis and biohydrogenation and their manipulation |
title_sort |
role of microbes in rumen lipolysis and biohydrogenation and their manipulation |
publisher |
Elsevier |
series |
Animal |
issn |
1751-7311 |
publishDate |
2010-01-01 |
description |
Despite the fact that the ruminant diet is rich in polyunsaturated fatty acids (PUFA), ruminant products – meat, milk and dairy – contain mainly saturated fatty acids (SFA) because of bacterial lipolysis and subsequent biohydrogenation of ingested PUFA in the rumen. The link between SFA consumption by man and coronary heart disease is well established. In contrast, ruminant products also contain fatty acids that are known to be beneficial to human health, namely conjugated linoleic acids (CLAs). The aims of research in this field have been to understand the microbial ecology of lipolysis and biohydrogenation and to find ways of manipulating ruminal microbes to increase the flow of PUFA and CLA from the rumen into meat and milk. This review describes our present understanding of the microbial ecology of ruminal lipid metabolism, including some apparently anomalous and paradoxical observations, and the status of how the metabolism may be manipulated and the possible consequential effects on other aspects of ruminal digestion. Intuitively, it may appear that inhibiting the ruminal lipase would cause more dietary PUFA to reach the mammary gland. However, lipolysis releases the non-esterified fatty acids that form the substrates for biohydrogenation, but which can, if they accumulate, inhibit the whole process. Thus, increasing lipase activity could be beneficial if the increased release of non-esterified PUFA inhibited the metabolism of CLA. Rumen ciliate protozoa do not carry out biohydrogenation, yet protozoal lipids are much more highly enriched in CLA than bacterial lipids. How could this happen if protozoa do not metabolise PUFA? The answer seems to lie in the ingestion of plant organelles, particularly chloroplasts, and the partial metabolism of the fatty acids by contaminating bacteria. Bacteria related to Butyrivibrio fibrisolvens are by far the most active and numerous biohydrogenating bacteria isolated from the rumen. But do we misunderstand the role of different bacterial species in biohydrogenation because there are uncultivated species that we need to understand and include in the analysis? Manipulation methods include dietary vegetable and fish oils and plant-derived chemicals. Their usefulness, efficacy and possible effects on fatty acid metabolism and on ruminal microorganisms and other areas of their metabolism are described, and areas of opportunity identified. |
topic |
biohydrogenation cellulose digestion lipase microbial protein synthesis rumen |
url |
http://www.sciencedirect.com/science/article/pii/S175173111000042X |
work_keys_str_mv |
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