Salmon cartilage proteoglycan promotes the healing process of Staphylococcus aureus-infected wound
Wound healing is the critical event for maintaining skin function and barrier. Inflammatory state in which a variety of cells are activated and accumulated is important for wound healing. Bacterial infection in cutaneous wound is a common problem and causes delay of wound healing. Our previous study...
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doaj-80e2c75bfc2c46269c90b67c95bee9fd2020-11-25T02:14:04ZengElsevierHeliyon2405-84402018-03-0143e00587Salmon cartilage proteoglycan promotes the healing process of Staphylococcus aureus-infected woundShouhei Hirose0Kouji Narita1Krisana Asano2Akio Nakane3Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan; Department of Biopolymer and Health Science, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, JapanDepartment of Microbiology and Immunology, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan; Institute for Animal Experimentation, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, JapanDepartment of Microbiology and Immunology, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan; Department of Biopolymer and Health Science, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan; Corresponding authors.Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan; Department of Biopolymer and Health Science, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan; Corresponding authors.Wound healing is the critical event for maintaining skin function and barrier. Inflammatory state in which a variety of cells are activated and accumulated is important for wound healing. Bacterial infection in cutaneous wound is a common problem and causes delay of wound healing. Our previous study demonstrated that the salmon nasal cartilage proteoglycan (PG) has an immunomodulatory effect in various mouse models of inflammatory disease. In this study, we investigated the effect of PG on healing process of Staphylococcus aureus-infected wound. PG accelerated wound closure in the initial phase of both infected and non-infected wound healing. In addition, the bacterial number in wounds of the PG-treated mice was significantly lower than that in the vehicle group. Neutrophil and macrophage infiltration was intensively observed in the PG-treated mice on day 2 after S. aureus inoculation, whereas neutrophil and macrophage influx was highly detected on day 6 in the vehicle control. Moreover, the production of TGF-β and IL-6 in the wound tissue was significantly promoted compared to the vehicle control on day 1. In contrast, the production of IL-1β and TNF-α in PG-treated mice was significantly decreased compared to the vehicle control on day 5. These data suggested that PG modulates the inflammatory state in infected wounds leading to promote wound healing.http://www.sciencedirect.com/science/article/pii/S2405844017330426MicrobiologyPharmaceutical chemistry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shouhei Hirose Kouji Narita Krisana Asano Akio Nakane |
spellingShingle |
Shouhei Hirose Kouji Narita Krisana Asano Akio Nakane Salmon cartilage proteoglycan promotes the healing process of Staphylococcus aureus-infected wound Heliyon Microbiology Pharmaceutical chemistry |
author_facet |
Shouhei Hirose Kouji Narita Krisana Asano Akio Nakane |
author_sort |
Shouhei Hirose |
title |
Salmon cartilage proteoglycan promotes the healing process of Staphylococcus aureus-infected wound |
title_short |
Salmon cartilage proteoglycan promotes the healing process of Staphylococcus aureus-infected wound |
title_full |
Salmon cartilage proteoglycan promotes the healing process of Staphylococcus aureus-infected wound |
title_fullStr |
Salmon cartilage proteoglycan promotes the healing process of Staphylococcus aureus-infected wound |
title_full_unstemmed |
Salmon cartilage proteoglycan promotes the healing process of Staphylococcus aureus-infected wound |
title_sort |
salmon cartilage proteoglycan promotes the healing process of staphylococcus aureus-infected wound |
publisher |
Elsevier |
series |
Heliyon |
issn |
2405-8440 |
publishDate |
2018-03-01 |
description |
Wound healing is the critical event for maintaining skin function and barrier. Inflammatory state in which a variety of cells are activated and accumulated is important for wound healing. Bacterial infection in cutaneous wound is a common problem and causes delay of wound healing. Our previous study demonstrated that the salmon nasal cartilage proteoglycan (PG) has an immunomodulatory effect in various mouse models of inflammatory disease. In this study, we investigated the effect of PG on healing process of Staphylococcus aureus-infected wound. PG accelerated wound closure in the initial phase of both infected and non-infected wound healing. In addition, the bacterial number in wounds of the PG-treated mice was significantly lower than that in the vehicle group. Neutrophil and macrophage infiltration was intensively observed in the PG-treated mice on day 2 after S. aureus inoculation, whereas neutrophil and macrophage influx was highly detected on day 6 in the vehicle control. Moreover, the production of TGF-β and IL-6 in the wound tissue was significantly promoted compared to the vehicle control on day 1. In contrast, the production of IL-1β and TNF-α in PG-treated mice was significantly decreased compared to the vehicle control on day 5. These data suggested that PG modulates the inflammatory state in infected wounds leading to promote wound healing. |
topic |
Microbiology Pharmaceutical chemistry |
url |
http://www.sciencedirect.com/science/article/pii/S2405844017330426 |
work_keys_str_mv |
AT shouheihirose salmoncartilageproteoglycanpromotesthehealingprocessofstaphylococcusaureusinfectedwound AT koujinarita salmoncartilageproteoglycanpromotesthehealingprocessofstaphylococcusaureusinfectedwound AT krisanaasano salmoncartilageproteoglycanpromotesthehealingprocessofstaphylococcusaureusinfectedwound AT akionakane salmoncartilageproteoglycanpromotesthehealingprocessofstaphylococcusaureusinfectedwound |
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1724902115030073344 |