New device for sperm preparation involving migration-gravity sedimentation without centrifugation compared with density-gradient centrifugation for normozoospermic intrauterine insemination
Objective: To investigate the efficacy of a new device for sperm preparation involving migration-gravity sedimentation without centrifugation (MIGLIS), compared with density-gradient centrifugation (DGC) for normozoospermic intrauterine insemination (IUI). Design: Retrospective cohort study. Setting...
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doaj-80efb205325b4f8ea669f2245bc704202021-03-18T04:42:35ZengElsevierF&S Reports2666-33412020-09-0112106112New device for sperm preparation involving migration-gravity sedimentation without centrifugation compared with density-gradient centrifugation for normozoospermic intrauterine inseminationKenichi Tatsumi, M.D., Ph.D.0Takayuki Tatsumi, M.D., Ph.D.1Takafumi Uchida, M.D.2Kazuki Saito, M.D., Ph.D.3Hidekazu Saito, M.D., Ph.D.4Umegaoka Women’s Clinic, Tokyo, JapanDepartment of Pediatrics, Perinatal and Maternal Medicine (Ibaraki), Graduate School, Tokyo Medical and Dental University, Tokyo, Japan; Reprint requests: Takayuki Tatsumi, M.D., Ph.D., Department of Pediatrics, Perinatal and Maternal Medicine, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.Umegaoka Women’s Clinic, Tokyo, JapanDepartment of Pediatrics, Perinatal and Maternal Medicine (Ibaraki), Graduate School, Tokyo Medical and Dental University, Tokyo, JapanUmegaoka Women’s Clinic, Tokyo, JapanObjective: To investigate the efficacy of a new device for sperm preparation involving migration-gravity sedimentation without centrifugation (MIGLIS), compared with density-gradient centrifugation (DGC) for normozoospermic intrauterine insemination (IUI). Design: Retrospective cohort study. Setting: Not applicable. Patients: A total of 10,318 cases of IUI (3,015 MIGLIS and 7,303 DGC) between October 2013 and September 2019. Interventions: None. Main Outcome Measures: Sperm analysis, subsequent pregnancy outcomes, and complications. Results: MIGLIS was associated with a lower sperm recovery rate and fewer injected sperm compared with DGC. However, the overall pregnancy rates following MIGLIS and DGC were similar (MIGLIS 8.8%, DGC 9.3%). In a subanalysis according to age, the pregnancy rate was higher for MIGLIS among women 40–41 years of age (8.6% vs. 5.9%). Peritonitis was the only recorded complication, with similar frequencies in the MIGLIS and DGC groups (MIGLIS two cases, DGC four cases). No cases became severe, and all improved after antibiotic treatment. There were no cases of uterine cramping or pain symptoms. Conclusions: MIGLIS is a new sperm preparation method that does not require centrifugation. Its use was associated with pregnancy rates similar to those with DGC and a higher pregnancy rate in older women. MIGLIS is a novel sperm preparation method for selecting spermatozoa with high motility and good fertilization ability in patients undergoing IUI, in vitro fertilization, and intracytoplasmic sperm injection.http://www.sciencedirect.com/science/article/pii/S2666334120300258MIGLIScentrifugationsperm preparationsperm DNAintrauterine insemination |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kenichi Tatsumi, M.D., Ph.D. Takayuki Tatsumi, M.D., Ph.D. Takafumi Uchida, M.D. Kazuki Saito, M.D., Ph.D. Hidekazu Saito, M.D., Ph.D. |
spellingShingle |
Kenichi Tatsumi, M.D., Ph.D. Takayuki Tatsumi, M.D., Ph.D. Takafumi Uchida, M.D. Kazuki Saito, M.D., Ph.D. Hidekazu Saito, M.D., Ph.D. New device for sperm preparation involving migration-gravity sedimentation without centrifugation compared with density-gradient centrifugation for normozoospermic intrauterine insemination F&S Reports MIGLIS centrifugation sperm preparation sperm DNA intrauterine insemination |
author_facet |
Kenichi Tatsumi, M.D., Ph.D. Takayuki Tatsumi, M.D., Ph.D. Takafumi Uchida, M.D. Kazuki Saito, M.D., Ph.D. Hidekazu Saito, M.D., Ph.D. |
author_sort |
Kenichi Tatsumi, M.D., Ph.D. |
title |
New device for sperm preparation involving migration-gravity sedimentation without centrifugation compared with density-gradient centrifugation for normozoospermic intrauterine insemination |
title_short |
New device for sperm preparation involving migration-gravity sedimentation without centrifugation compared with density-gradient centrifugation for normozoospermic intrauterine insemination |
title_full |
New device for sperm preparation involving migration-gravity sedimentation without centrifugation compared with density-gradient centrifugation for normozoospermic intrauterine insemination |
title_fullStr |
New device for sperm preparation involving migration-gravity sedimentation without centrifugation compared with density-gradient centrifugation for normozoospermic intrauterine insemination |
title_full_unstemmed |
New device for sperm preparation involving migration-gravity sedimentation without centrifugation compared with density-gradient centrifugation for normozoospermic intrauterine insemination |
title_sort |
new device for sperm preparation involving migration-gravity sedimentation without centrifugation compared with density-gradient centrifugation for normozoospermic intrauterine insemination |
publisher |
Elsevier |
series |
F&S Reports |
issn |
2666-3341 |
publishDate |
2020-09-01 |
description |
Objective: To investigate the efficacy of a new device for sperm preparation involving migration-gravity sedimentation without centrifugation (MIGLIS), compared with density-gradient centrifugation (DGC) for normozoospermic intrauterine insemination (IUI). Design: Retrospective cohort study. Setting: Not applicable. Patients: A total of 10,318 cases of IUI (3,015 MIGLIS and 7,303 DGC) between October 2013 and September 2019. Interventions: None. Main Outcome Measures: Sperm analysis, subsequent pregnancy outcomes, and complications. Results: MIGLIS was associated with a lower sperm recovery rate and fewer injected sperm compared with DGC. However, the overall pregnancy rates following MIGLIS and DGC were similar (MIGLIS 8.8%, DGC 9.3%). In a subanalysis according to age, the pregnancy rate was higher for MIGLIS among women 40–41 years of age (8.6% vs. 5.9%). Peritonitis was the only recorded complication, with similar frequencies in the MIGLIS and DGC groups (MIGLIS two cases, DGC four cases). No cases became severe, and all improved after antibiotic treatment. There were no cases of uterine cramping or pain symptoms. Conclusions: MIGLIS is a new sperm preparation method that does not require centrifugation. Its use was associated with pregnancy rates similar to those with DGC and a higher pregnancy rate in older women. MIGLIS is a novel sperm preparation method for selecting spermatozoa with high motility and good fertilization ability in patients undergoing IUI, in vitro fertilization, and intracytoplasmic sperm injection. |
topic |
MIGLIS centrifugation sperm preparation sperm DNA intrauterine insemination |
url |
http://www.sciencedirect.com/science/article/pii/S2666334120300258 |
work_keys_str_mv |
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