The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa
Introduction: Antibiotic resistance crisis has always been a serious problem for human health and many hospitalized patients are affected worldwide. Pseudomonas aeruginosa is a gram-negative pathogen and one of the most common causes of nosocomial infections. The main mechanism of resistance to beta...
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Ilam University of Medical Sciences
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doaj-80f1bc49bfd4491f9ce98e378be6cd862020-11-24T22:20:19Zfas Ilam University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām1563-47282588-31352018-11-012644352The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosaRahele Naserian Moghadam0Fatemeh Roodbari1Mahboubeh Naderi Nasab2Davoud Mansouri3Seyedeh Zohre Mirbagheri4Aida Gholoobi5Amin Hooshyar chichaklu6Zahra Meshkat7 Mazandaran University Dept of Cell and Molecular Biology, Faculty of Basic Sciences, Mazandaran University, Mazandaran, Iran Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Dept of Microbiology, Tehran University of Medical Sciences, Tehran, Iran Dept of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Introduction: Antibiotic resistance crisis has always been a serious problem for human health and many hospitalized patients are affected worldwide. Pseudomonas aeruginosa is a gram-negative pathogen and one of the most common causes of nosocomial infections. The main mechanism of resistance to beta-lactam antibiotics is the presence of metallo-beta-lactamase (MBL) enzymes. Most of the MBL genes are found in plasmids. The aim of this study was to evaluate the frequency of MBL-producing P. aeruginosa isolates caused by VIM-all and VIM 2, 3, 9, 11and16 genes. Materials & Methods: Antimicrobial susceptibility of 127 clinical isolates of P. aeruginosa was determined using the standard Kirby-Bauer disk diffusion method according to Clinical and Laboratory Standard Institute (CLSI). Combined-disk test was used for phenotypic determination of MBLs-producing isolates. After DNA extraction, VIM-all and in specific, VIM 2, 3, 9, 11 and 16 genes were amplified using PCR method. Findings: A total Of 127 clinical isolates of P. aeruginosa, 62 isolates (49%) were resistant to imipenem and 31 isolates (24.5%) showed phenotypic evidences of MBL production. Moreover, among imipenem resistant strains VIM-all genes were found in 12.5% of cases, but the VIM 2-3-9-11 and 16 genes were not detected in samples. Discussion & Conclusions: The results obtained in this study suggest that in P. aeruginosa, the highest antibiotic resistance observed was to cefazolin (98%) followed by nalidixic acid (91%) and the least resistance were to ciprofloxacin (31%). One of the reasons for this trend is the growth of antibiotic-resistant bacteria and the known mechanisms of bacterial resistance.http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-3667-1&slc_lang=en&sid=1Metallo-beta-lactamasePseudomonas aeruginosaAntibiotic resistanceVIM gene |
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language |
fas |
format |
Article |
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DOAJ |
author |
Rahele Naserian Moghadam Fatemeh Roodbari Mahboubeh Naderi Nasab Davoud Mansouri Seyedeh Zohre Mirbagheri Aida Gholoobi Amin Hooshyar chichaklu Zahra Meshkat |
spellingShingle |
Rahele Naserian Moghadam Fatemeh Roodbari Mahboubeh Naderi Nasab Davoud Mansouri Seyedeh Zohre Mirbagheri Aida Gholoobi Amin Hooshyar chichaklu Zahra Meshkat The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām Metallo-beta-lactamase Pseudomonas aeruginosa Antibiotic resistance VIM gene |
author_facet |
Rahele Naserian Moghadam Fatemeh Roodbari Mahboubeh Naderi Nasab Davoud Mansouri Seyedeh Zohre Mirbagheri Aida Gholoobi Amin Hooshyar chichaklu Zahra Meshkat |
author_sort |
Rahele Naserian Moghadam |
title |
The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa |
title_short |
The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa |
title_full |
The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa |
title_fullStr |
The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa |
title_full_unstemmed |
The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa |
title_sort |
frequency of vim 2, 3, 9, 11 and vim all among metallo-beta-lactamase producing pseudomonas aeruginosa |
publisher |
Ilam University of Medical Sciences |
series |
Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
issn |
1563-4728 2588-3135 |
publishDate |
2018-11-01 |
description |
Introduction: Antibiotic resistance crisis has always been a serious problem for human health and many hospitalized patients are affected worldwide. Pseudomonas aeruginosa is a gram-negative pathogen and one of the most common causes of nosocomial infections. The main mechanism of resistance to beta-lactam antibiotics is the presence of metallo-beta-lactamase (MBL) enzymes. Most of the MBL genes are found in plasmids. The aim of this study was to evaluate the frequency of MBL-producing P. aeruginosa isolates caused by VIM-all and VIM 2, 3, 9, 11and16 genes.
Materials & Methods: Antimicrobial susceptibility of 127 clinical isolates of P. aeruginosa was determined using the standard Kirby-Bauer disk diffusion method according to Clinical and Laboratory Standard Institute (CLSI). Combined-disk test was used for phenotypic determination of MBLs-producing isolates. After DNA extraction, VIM-all and in specific, VIM 2, 3, 9, 11 and 16 genes were amplified using PCR method.
Findings: A total Of 127 clinical isolates of P. aeruginosa, 62 isolates (49%) were resistant to imipenem and 31 isolates (24.5%) showed phenotypic evidences of MBL production. Moreover, among imipenem resistant strains VIM-all genes were found in 12.5% of cases, but the VIM 2-3-9-11 and 16 genes were not detected in samples.
Discussion & Conclusions: The results obtained in this study suggest that in P. aeruginosa, the highest antibiotic resistance observed was to cefazolin (98%) followed by nalidixic acid (91%) and the least resistance were to ciprofloxacin (31%). One of the reasons for this trend is the growth of antibiotic-resistant bacteria and the known mechanisms of bacterial resistance. |
topic |
Metallo-beta-lactamase Pseudomonas aeruginosa Antibiotic resistance VIM gene |
url |
http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-3667-1&slc_lang=en&sid=1 |
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