The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa

Introduction: Antibiotic resistance crisis has always been a serious problem for human health and many hospitalized patients are affected worldwide. Pseudomonas aeruginosa is a gram-negative pathogen and one of the most common causes of nosocomial infections. The main mechanism of resistance to beta...

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Main Authors: Rahele Naserian Moghadam, Fatemeh Roodbari, Mahboubeh Naderi Nasab, Davoud Mansouri, Seyedeh Zohre Mirbagheri, Aida Gholoobi, Amin Hooshyar chichaklu, Zahra Meshkat
Format: Article
Language:fas
Published: Ilam University of Medical Sciences 2018-11-01
Series:Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām
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Online Access:http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-3667-1&slc_lang=en&sid=1
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spelling doaj-80f1bc49bfd4491f9ce98e378be6cd862020-11-24T22:20:19Zfas Ilam University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām1563-47282588-31352018-11-012644352The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosaRahele Naserian Moghadam0Fatemeh Roodbari1Mahboubeh Naderi Nasab2Davoud Mansouri3Seyedeh Zohre Mirbagheri4Aida Gholoobi5Amin Hooshyar chichaklu6Zahra Meshkat7 Mazandaran University Dept of Cell and Molecular Biology, Faculty of Basic Sciences, Mazandaran University, Mazandaran, Iran Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Dept of Microbiology, Tehran University of Medical Sciences, Tehran, Iran Dept of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Introduction: Antibiotic resistance crisis has always been a serious problem for human health and many hospitalized patients are affected worldwide. Pseudomonas aeruginosa is a gram-negative pathogen and one of the most common causes of nosocomial infections. The main mechanism of resistance to beta-lactam antibiotics is the presence of metallo-beta-lactamase (MBL) enzymes. Most of the MBL genes are found in plasmids. The aim of this study was to evaluate the frequency of MBL-producing P. aeruginosa isolates caused by VIM-all and VIM 2, 3, 9, 11and16 genes.   Materials & Methods: Antimicrobial susceptibility of 127 clinical isolates of P. aeruginosa was determined using the standard Kirby-Bauer disk diffusion method according to Clinical and Laboratory Standard Institute (CLSI). Combined-disk test was used for phenotypic determination of MBLs-producing isolates. After DNA extraction, VIM-all and in specific, VIM 2, 3, 9, 11 and 16 genes were amplified using PCR method.   Findings: A total Of 127 clinical isolates of P. aeruginosa, 62 isolates (49%) were resistant to imipenem and 31 isolates (24.5%) showed phenotypic evidences of MBL production. Moreover, among imipenem resistant strains VIM-all genes were found in 12.5% of cases, but the VIM 2-3-9-11 and 16 genes were not detected in samples.   Discussion & Conclusions: The results obtained in this study suggest that in P. aeruginosa, the highest antibiotic resistance observed was to cefazolin (98%) followed by nalidixic acid (91%) and the least resistance were to ciprofloxacin (31%). One of the reasons for this trend is the growth of antibiotic-resistant bacteria and the known mechanisms of bacterial resistance.http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-3667-1&slc_lang=en&sid=1Metallo-beta-lactamasePseudomonas aeruginosaAntibiotic resistanceVIM gene
collection DOAJ
language fas
format Article
sources DOAJ
author Rahele Naserian Moghadam
Fatemeh Roodbari
Mahboubeh Naderi Nasab
Davoud Mansouri
Seyedeh Zohre Mirbagheri
Aida Gholoobi
Amin Hooshyar chichaklu
Zahra Meshkat
spellingShingle Rahele Naserian Moghadam
Fatemeh Roodbari
Mahboubeh Naderi Nasab
Davoud Mansouri
Seyedeh Zohre Mirbagheri
Aida Gholoobi
Amin Hooshyar chichaklu
Zahra Meshkat
The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa
Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām
Metallo-beta-lactamase
Pseudomonas aeruginosa
Antibiotic resistance
VIM gene
author_facet Rahele Naserian Moghadam
Fatemeh Roodbari
Mahboubeh Naderi Nasab
Davoud Mansouri
Seyedeh Zohre Mirbagheri
Aida Gholoobi
Amin Hooshyar chichaklu
Zahra Meshkat
author_sort Rahele Naserian Moghadam
title The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa
title_short The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa
title_full The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa
title_fullStr The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa
title_full_unstemmed The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa
title_sort frequency of vim 2, 3, 9, 11 and vim all among metallo-beta-lactamase producing pseudomonas aeruginosa
publisher Ilam University of Medical Sciences
series Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām
issn 1563-4728
2588-3135
publishDate 2018-11-01
description Introduction: Antibiotic resistance crisis has always been a serious problem for human health and many hospitalized patients are affected worldwide. Pseudomonas aeruginosa is a gram-negative pathogen and one of the most common causes of nosocomial infections. The main mechanism of resistance to beta-lactam antibiotics is the presence of metallo-beta-lactamase (MBL) enzymes. Most of the MBL genes are found in plasmids. The aim of this study was to evaluate the frequency of MBL-producing P. aeruginosa isolates caused by VIM-all and VIM 2, 3, 9, 11and16 genes.   Materials & Methods: Antimicrobial susceptibility of 127 clinical isolates of P. aeruginosa was determined using the standard Kirby-Bauer disk diffusion method according to Clinical and Laboratory Standard Institute (CLSI). Combined-disk test was used for phenotypic determination of MBLs-producing isolates. After DNA extraction, VIM-all and in specific, VIM 2, 3, 9, 11 and 16 genes were amplified using PCR method.   Findings: A total Of 127 clinical isolates of P. aeruginosa, 62 isolates (49%) were resistant to imipenem and 31 isolates (24.5%) showed phenotypic evidences of MBL production. Moreover, among imipenem resistant strains VIM-all genes were found in 12.5% of cases, but the VIM 2-3-9-11 and 16 genes were not detected in samples.   Discussion & Conclusions: The results obtained in this study suggest that in P. aeruginosa, the highest antibiotic resistance observed was to cefazolin (98%) followed by nalidixic acid (91%) and the least resistance were to ciprofloxacin (31%). One of the reasons for this trend is the growth of antibiotic-resistant bacteria and the known mechanisms of bacterial resistance.
topic Metallo-beta-lactamase
Pseudomonas aeruginosa
Antibiotic resistance
VIM gene
url http://sjimu.medilam.ac.ir/browse.php?a_code=A-10-3667-1&slc_lang=en&sid=1
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