Pathogenicity of indigenous soil isolate of Bacillus thuringiensis to Helicoverpa armigera Hübner 1809 (Lepidoptera: Noctuidae)
Abstract This study evaluated the pathogenicity of indigenous soil isolate of Bacillus thuringiensis (Bt) strains, applied without and along with 1.0% MgCl2 salt, to Helicoverpa armigera Hübner (Lepidoptera: Noctuidae). Toxicity and effect of Bt isolate on larval development (weight) were assessed u...
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doaj-8100a87fc39c49cc9af3d2b84d751f472020-11-24T21:50:38ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422018-04-012811710.1186/s41938-018-0041-4Pathogenicity of indigenous soil isolate of Bacillus thuringiensis to Helicoverpa armigera Hübner 1809 (Lepidoptera: Noctuidae)Muhammad Zeeshan Majeed0Muhammad Asam Riaz1Muneeba Arif Khan2Chun-Sen Ma3Salman Ahmad4State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesDepartment of Entomology, College of Agriculture, University of SargodhaDepartment of Entomology, College of Agriculture, University of SargodhaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesDepartment of Plant Pathology, College of Agriculture, University of SargodhaAbstract This study evaluated the pathogenicity of indigenous soil isolate of Bacillus thuringiensis (Bt) strains, applied without and along with 1.0% MgCl2 salt, to Helicoverpa armigera Hübner (Lepidoptera: Noctuidae). Toxicity and effect of Bt isolate on larval development (weight) were assessed using in vitro bioassays. Six concentrations of the tested Bt with salt (i.e., 1.0 × 107, 0.5 × 105, 1.0 × 105, 1.5 × 105, and 2.0 × 105 cfu/ml), five without salt (i.e., 0.5 × 105, 1.0 × 105, 1.5 × 105, and 2.0 × 105 cfu/ml), and control were bioassayed against third-instar larvae of H. armigera under a complete randomized design (CRD), with four replications. Results revealed that both larval mortality and weight changes were significantly affected by time (F 5, 19 = 35.98; P < 0.001 and F 5, 19 = 11.01; P < 0.001, respectively) and treatments (F 5, 19 = 27.45; P < 0.001 and F 5, 19 = 25.07; P < 0.001). The highest larval mortality (91.1%) was exhibited by the highest concentration (1.0 × 107 cfu/ml), followed by 2.0 × 105 cfu/ml concentration, without salt (88.9%) and with salt (66.7%). Median lethal concentration (LC50) values of isolated Bt strain were 1.7 and 1.27 × 105 cfu/ml, without salt, and 1.80 and 1.13 × 105 cfu/ml, with salt, at 96 and 120 h, respectively. Regarding the impact of Bt isolate on larval development, treatment with the highest concentration (1.0 × 107 cfu/ml) had the most significant and negative impact on larval weight change (R 2 = 0.53), followed by 2.0 × 105 cfu/ml Bt concentration (R 2 = 0.40). There was no obvious synergistic or additive effect, but rather an inhibition was observed on the pathogenicity potential or larvicidal effect of Bt isolate.http://link.springer.com/article/10.1186/s41938-018-0041-4Bacillus thuringiensisMicrobial insecticidesHelicoverpa armigeraToxicity evaluation |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Muhammad Zeeshan Majeed Muhammad Asam Riaz Muneeba Arif Khan Chun-Sen Ma Salman Ahmad |
spellingShingle |
Muhammad Zeeshan Majeed Muhammad Asam Riaz Muneeba Arif Khan Chun-Sen Ma Salman Ahmad Pathogenicity of indigenous soil isolate of Bacillus thuringiensis to Helicoverpa armigera Hübner 1809 (Lepidoptera: Noctuidae) Egyptian Journal of Biological Pest Control Bacillus thuringiensis Microbial insecticides Helicoverpa armigera Toxicity evaluation |
author_facet |
Muhammad Zeeshan Majeed Muhammad Asam Riaz Muneeba Arif Khan Chun-Sen Ma Salman Ahmad |
author_sort |
Muhammad Zeeshan Majeed |
title |
Pathogenicity of indigenous soil isolate of Bacillus thuringiensis to Helicoverpa armigera Hübner 1809 (Lepidoptera: Noctuidae) |
title_short |
Pathogenicity of indigenous soil isolate of Bacillus thuringiensis to Helicoverpa armigera Hübner 1809 (Lepidoptera: Noctuidae) |
title_full |
Pathogenicity of indigenous soil isolate of Bacillus thuringiensis to Helicoverpa armigera Hübner 1809 (Lepidoptera: Noctuidae) |
title_fullStr |
Pathogenicity of indigenous soil isolate of Bacillus thuringiensis to Helicoverpa armigera Hübner 1809 (Lepidoptera: Noctuidae) |
title_full_unstemmed |
Pathogenicity of indigenous soil isolate of Bacillus thuringiensis to Helicoverpa armigera Hübner 1809 (Lepidoptera: Noctuidae) |
title_sort |
pathogenicity of indigenous soil isolate of bacillus thuringiensis to helicoverpa armigera hübner 1809 (lepidoptera: noctuidae) |
publisher |
SpringerOpen |
series |
Egyptian Journal of Biological Pest Control |
issn |
2536-9342 |
publishDate |
2018-04-01 |
description |
Abstract This study evaluated the pathogenicity of indigenous soil isolate of Bacillus thuringiensis (Bt) strains, applied without and along with 1.0% MgCl2 salt, to Helicoverpa armigera Hübner (Lepidoptera: Noctuidae). Toxicity and effect of Bt isolate on larval development (weight) were assessed using in vitro bioassays. Six concentrations of the tested Bt with salt (i.e., 1.0 × 107, 0.5 × 105, 1.0 × 105, 1.5 × 105, and 2.0 × 105 cfu/ml), five without salt (i.e., 0.5 × 105, 1.0 × 105, 1.5 × 105, and 2.0 × 105 cfu/ml), and control were bioassayed against third-instar larvae of H. armigera under a complete randomized design (CRD), with four replications. Results revealed that both larval mortality and weight changes were significantly affected by time (F 5, 19 = 35.98; P < 0.001 and F 5, 19 = 11.01; P < 0.001, respectively) and treatments (F 5, 19 = 27.45; P < 0.001 and F 5, 19 = 25.07; P < 0.001). The highest larval mortality (91.1%) was exhibited by the highest concentration (1.0 × 107 cfu/ml), followed by 2.0 × 105 cfu/ml concentration, without salt (88.9%) and with salt (66.7%). Median lethal concentration (LC50) values of isolated Bt strain were 1.7 and 1.27 × 105 cfu/ml, without salt, and 1.80 and 1.13 × 105 cfu/ml, with salt, at 96 and 120 h, respectively. Regarding the impact of Bt isolate on larval development, treatment with the highest concentration (1.0 × 107 cfu/ml) had the most significant and negative impact on larval weight change (R 2 = 0.53), followed by 2.0 × 105 cfu/ml Bt concentration (R 2 = 0.40). There was no obvious synergistic or additive effect, but rather an inhibition was observed on the pathogenicity potential or larvicidal effect of Bt isolate. |
topic |
Bacillus thuringiensis Microbial insecticides Helicoverpa armigera Toxicity evaluation |
url |
http://link.springer.com/article/10.1186/s41938-018-0041-4 |
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