Gut Microbiota in Patients with Different Metabolic Statuses: Moscow Study
The aim of this paper was to study gut microbiota composition in patients with different metabolic statuses. Methods: 92 participants aged 25–76 years (26 of whom were men), with confirmed absence of cardiovascular and other chronic diseases (but with the possible presence of cardiovascula...
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doaj-8100d9ad68d04acdbfcc07c37354fa4a2020-11-24T21:46:47ZengMDPI AGMicroorganisms2076-26072018-09-01649810.3390/microorganisms6040098microorganisms6040098Gut Microbiota in Patients with Different Metabolic Statuses: Moscow StudyDaria A. Kashtanova0Olga N. Tkacheva1Ekaterina N. Doudinskaya2Irina D. Strazhesko3Yulia V. Kotovskaya4Anna S. Popenko5Alexander V. Tyakht6Dmitry G. Alexeev7Russian Clinical Research Center for Gerontology, Pirogov Russian National Research Medical University of the Ministry of Healthcare of the Russian Federation, bld. 16, 1st Leonova Street, Moscow 129226, RussiaRussian Clinical Research Center for Gerontology, Pirogov Russian National Research Medical University of the Ministry of Healthcare of the Russian Federation, bld. 16, 1st Leonova Street, Moscow 129226, RussiaRussian Clinical Research Center for Gerontology, Pirogov Russian National Research Medical University of the Ministry of Healthcare of the Russian Federation, bld. 16, 1st Leonova Street, Moscow 129226, RussiaMedical Research and Education Center, Lomonosov Moscow State University, 1 Leninskie Gory, Moscow 119991, RussiaRussian Clinical Research Center for Gerontology, Pirogov Russian National Research Medical University of the Ministry of Healthcare of the Russian Federation, bld. 16, 1st Leonova Street, Moscow 129226, RussiaAtlas Biomed Group, Tintagel House, 92 Albert Embankment, Lambeth, London SE1 7TP, UKAtlas Biomed Group, Tintagel House, 92 Albert Embankment, Lambeth, London SE1 7TP, UKAtlas Biomed Group, Tintagel House, 92 Albert Embankment, Lambeth, London SE1 7TP, UKThe aim of this paper was to study gut microbiota composition in patients with different metabolic statuses. Methods: 92 participants aged 25–76 years (26 of whom were men), with confirmed absence of cardiovascular and other chronic diseases (but with the possible presence of cardiovascular risk factors) were included. Carotid ultrasound examinations, 16S rRNA sequencing of stool samples and diet assessments were performed. Statistical analysis was performed using R programming language, 3.1.0. Results: Enterotyping yielded two clusters differentiated by alpha-diversity. Intima-media thickness was higher in the cluster with lower diversity (adj. p < 0.001). Obesity was associated with higher Serratia (adj. p = 0.003) and Prevotella (adj. p < 0.0003) in relative abundance. Abdominal obesity was associated with higher abundance of Serratia (adj. p = 0.004) and Prevotella (adj. p = 0.0008) and lower levels of Oscillospira (adj. p = 0.0005). Glucose metabolism disturbances were associated with higher Blautia (adj. p = 0.0007) and Serratia (adj. p = 0.003) prevalence. Arterial hypertension was associated with high Blautia levels (adj. p = 0.002). The Blautia genus strongly correlated with low resistant starch consumption (adj. p = 0.007). A combination of high-fat diet and elevated Blautia levels was very common for diabetes mellitus type 2 patients (adj. p = 0.0001). Conclusion: The results show that there is a relationship between metabolic changes and higher representation of opportunistic pathogens and low diversity of gut microbiota even in apparently healthy participants.http://www.mdpi.com/2076-2607/6/4/98gut microbiotametabolic statusglucose metabolismcardiovascular risk factorsdiet |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daria A. Kashtanova Olga N. Tkacheva Ekaterina N. Doudinskaya Irina D. Strazhesko Yulia V. Kotovskaya Anna S. Popenko Alexander V. Tyakht Dmitry G. Alexeev |
spellingShingle |
Daria A. Kashtanova Olga N. Tkacheva Ekaterina N. Doudinskaya Irina D. Strazhesko Yulia V. Kotovskaya Anna S. Popenko Alexander V. Tyakht Dmitry G. Alexeev Gut Microbiota in Patients with Different Metabolic Statuses: Moscow Study Microorganisms gut microbiota metabolic status glucose metabolism cardiovascular risk factors diet |
author_facet |
Daria A. Kashtanova Olga N. Tkacheva Ekaterina N. Doudinskaya Irina D. Strazhesko Yulia V. Kotovskaya Anna S. Popenko Alexander V. Tyakht Dmitry G. Alexeev |
author_sort |
Daria A. Kashtanova |
title |
Gut Microbiota in Patients with Different Metabolic Statuses: Moscow Study |
title_short |
Gut Microbiota in Patients with Different Metabolic Statuses: Moscow Study |
title_full |
Gut Microbiota in Patients with Different Metabolic Statuses: Moscow Study |
title_fullStr |
Gut Microbiota in Patients with Different Metabolic Statuses: Moscow Study |
title_full_unstemmed |
Gut Microbiota in Patients with Different Metabolic Statuses: Moscow Study |
title_sort |
gut microbiota in patients with different metabolic statuses: moscow study |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2018-09-01 |
description |
The aim of this paper was to study gut microbiota composition in patients with different metabolic statuses. Methods: 92 participants aged 25–76 years (26 of whom were men), with confirmed absence of cardiovascular and other chronic diseases (but with the possible presence of cardiovascular risk factors) were included. Carotid ultrasound examinations, 16S rRNA sequencing of stool samples and diet assessments were performed. Statistical analysis was performed using R programming language, 3.1.0. Results: Enterotyping yielded two clusters differentiated by alpha-diversity. Intima-media thickness was higher in the cluster with lower diversity (adj. p < 0.001). Obesity was associated with higher Serratia (adj. p = 0.003) and Prevotella (adj. p < 0.0003) in relative abundance. Abdominal obesity was associated with higher abundance of Serratia (adj. p = 0.004) and Prevotella (adj. p = 0.0008) and lower levels of Oscillospira (adj. p = 0.0005). Glucose metabolism disturbances were associated with higher Blautia (adj. p = 0.0007) and Serratia (adj. p = 0.003) prevalence. Arterial hypertension was associated with high Blautia levels (adj. p = 0.002). The Blautia genus strongly correlated with low resistant starch consumption (adj. p = 0.007). A combination of high-fat diet and elevated Blautia levels was very common for diabetes mellitus type 2 patients (adj. p = 0.0001). Conclusion: The results show that there is a relationship between metabolic changes and higher representation of opportunistic pathogens and low diversity of gut microbiota even in apparently healthy participants. |
topic |
gut microbiota metabolic status glucose metabolism cardiovascular risk factors diet |
url |
http://www.mdpi.com/2076-2607/6/4/98 |
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