Cellular and Extracellular Proteome of the Animal Pathogen <i>Corynebacterium silvaticum</i>, a Close Relative of Zoonotic <i>Corynebacterium ulcerans</i> and <i>Corynebacterium pseudotuberculosis</i>
<i>Corynebacterium silvaticum</i> is a newly described animal pathogen, closely related to the emerging human pathogen <i>Corynebacterium ulcerans</i> and <i>Corynebacterium pseudotuberculosis</i>, a major pathogen of small ruminants. In this study, proteins of a...
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doaj-8117cf99aadb45aa9578e05979aa1d742020-11-25T03:20:49ZengMDPI AGProteomes2227-73822020-08-018191910.3390/proteomes8030019Cellular and Extracellular Proteome of the Animal Pathogen <i>Corynebacterium silvaticum</i>, a Close Relative of Zoonotic <i>Corynebacterium ulcerans</i> and <i>Corynebacterium pseudotuberculosis</i>Jens Möller0Svenja Schorlemmer1Jörg Hofmann2Andreas Burkovski3Microbiology Division, Department of Biology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, GermanyMicrobiology Division, Department of Biology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, GermanyBiochemistry Division, Department of Biology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, GermanyMicrobiology Division, Department of Biology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany<i>Corynebacterium silvaticum</i> is a newly described animal pathogen, closely related to the emerging human pathogen <i>Corynebacterium ulcerans</i> and <i>Corynebacterium pseudotuberculosis</i>, a major pathogen of small ruminants. In this study, proteins of a whole cell and a shaving fraction and the exoproteome of <i>C. silvaticum</i> strain W25 were analyzed as a first proteome study of this species. In total, 1305 proteins were identified out of 2013 proteins encoded by the W25 genome sequence and number of putative virulence factors were detected already under standard growth conditions including phospholipase D and sialidase. An up to now uncharacterized trypsin-like protease is by far the most secreted protein in this species, indicating a putative role in pathogenicity. Furthermore, the proteome analyses carried out in this study support the recently published taxonomical delineation of <i>C. silvaticum</i> from the closely related zoonotic <i>Corynebacterium</i> species.https://www.mdpi.com/2227-7382/8/3/19exoproteomeLC-MS/MSmass spectrometrynecrotizing lymphadenitissecretometrypsin-like serine protease |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jens Möller Svenja Schorlemmer Jörg Hofmann Andreas Burkovski |
spellingShingle |
Jens Möller Svenja Schorlemmer Jörg Hofmann Andreas Burkovski Cellular and Extracellular Proteome of the Animal Pathogen <i>Corynebacterium silvaticum</i>, a Close Relative of Zoonotic <i>Corynebacterium ulcerans</i> and <i>Corynebacterium pseudotuberculosis</i> Proteomes exoproteome LC-MS/MS mass spectrometry necrotizing lymphadenitis secretome trypsin-like serine protease |
author_facet |
Jens Möller Svenja Schorlemmer Jörg Hofmann Andreas Burkovski |
author_sort |
Jens Möller |
title |
Cellular and Extracellular Proteome of the Animal Pathogen <i>Corynebacterium silvaticum</i>, a Close Relative of Zoonotic <i>Corynebacterium ulcerans</i> and <i>Corynebacterium pseudotuberculosis</i> |
title_short |
Cellular and Extracellular Proteome of the Animal Pathogen <i>Corynebacterium silvaticum</i>, a Close Relative of Zoonotic <i>Corynebacterium ulcerans</i> and <i>Corynebacterium pseudotuberculosis</i> |
title_full |
Cellular and Extracellular Proteome of the Animal Pathogen <i>Corynebacterium silvaticum</i>, a Close Relative of Zoonotic <i>Corynebacterium ulcerans</i> and <i>Corynebacterium pseudotuberculosis</i> |
title_fullStr |
Cellular and Extracellular Proteome of the Animal Pathogen <i>Corynebacterium silvaticum</i>, a Close Relative of Zoonotic <i>Corynebacterium ulcerans</i> and <i>Corynebacterium pseudotuberculosis</i> |
title_full_unstemmed |
Cellular and Extracellular Proteome of the Animal Pathogen <i>Corynebacterium silvaticum</i>, a Close Relative of Zoonotic <i>Corynebacterium ulcerans</i> and <i>Corynebacterium pseudotuberculosis</i> |
title_sort |
cellular and extracellular proteome of the animal pathogen <i>corynebacterium silvaticum</i>, a close relative of zoonotic <i>corynebacterium ulcerans</i> and <i>corynebacterium pseudotuberculosis</i> |
publisher |
MDPI AG |
series |
Proteomes |
issn |
2227-7382 |
publishDate |
2020-08-01 |
description |
<i>Corynebacterium silvaticum</i> is a newly described animal pathogen, closely related to the emerging human pathogen <i>Corynebacterium ulcerans</i> and <i>Corynebacterium pseudotuberculosis</i>, a major pathogen of small ruminants. In this study, proteins of a whole cell and a shaving fraction and the exoproteome of <i>C. silvaticum</i> strain W25 were analyzed as a first proteome study of this species. In total, 1305 proteins were identified out of 2013 proteins encoded by the W25 genome sequence and number of putative virulence factors were detected already under standard growth conditions including phospholipase D and sialidase. An up to now uncharacterized trypsin-like protease is by far the most secreted protein in this species, indicating a putative role in pathogenicity. Furthermore, the proteome analyses carried out in this study support the recently published taxonomical delineation of <i>C. silvaticum</i> from the closely related zoonotic <i>Corynebacterium</i> species. |
topic |
exoproteome LC-MS/MS mass spectrometry necrotizing lymphadenitis secretome trypsin-like serine protease |
url |
https://www.mdpi.com/2227-7382/8/3/19 |
work_keys_str_mv |
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