Segmented flow generator for serial crystallography at the European X-ray free electron laser
Due to the pulsed nature of X-ray free electron laser (XFEL) instruments the majority of protein crystals, which are injected using continuous jet injection techniques are wasted. Here, the authors present a microfluidic device to deliver aqueous protein crystal laden droplets segmented with an immi...
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Format: | Article |
Language: | English |
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Nature Publishing Group
2020-09-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-020-18156-7 |
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language |
English |
format |
Article |
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author |
Austin Echelmeier Jorvani Cruz Villarreal Marc Messerschmidt Daihyun Kim Jesse D. Coe Darren Thifault Sabine Botha Ana Egatz-Gomez Sahir Gandhi Gerrit Brehm Chelsie E. Conrad Debra T. Hansen Caleb Madsen Saša Bajt J. Domingo Meza-Aguilar Dominik Oberthür Max O. Wiedorn Holger Fleckenstein Derek Mendez Juraj Knoška Jose M. Martin-Garcia Hao Hu Stella Lisova Aschkan Allahgholi Yaroslav Gevorkov Kartik Ayyer Steve Aplin Helen Mary Ginn Heinz Graafsma Andrew J. Morgan Dominic Greiffenberg Alexander Klujev Torsten Laurus Jennifer Poehlsen Ulrich Trunk Davide Mezza Bernd Schmidt Manuela Kuhn Raimund Fromme Jolanta Sztuk-Dambietz Natascha Raab Steffen Hauf Alessandro Silenzi Thomas Michelat Chen Xu Cyril Danilevski Andrea Parenti Leonce Mekinda Britta Weinhausen Grant Mills Patrik Vagovic Yoonhee Kim Henry Kirkwood Richard Bean Johan Bielecki Stephan Stern Klaus Giewekemeyer Adam R. Round Joachim Schulz Katerina Dörner Thomas D. Grant Valerio Mariani Anton Barty Adrian P. Mancuso Uwe Weierstall John C. H. Spence Henry N. Chapman Nadia Zatsepin Petra Fromme Richard A. Kirian Alexandra Ros |
spellingShingle |
Austin Echelmeier Jorvani Cruz Villarreal Marc Messerschmidt Daihyun Kim Jesse D. Coe Darren Thifault Sabine Botha Ana Egatz-Gomez Sahir Gandhi Gerrit Brehm Chelsie E. Conrad Debra T. Hansen Caleb Madsen Saša Bajt J. Domingo Meza-Aguilar Dominik Oberthür Max O. Wiedorn Holger Fleckenstein Derek Mendez Juraj Knoška Jose M. Martin-Garcia Hao Hu Stella Lisova Aschkan Allahgholi Yaroslav Gevorkov Kartik Ayyer Steve Aplin Helen Mary Ginn Heinz Graafsma Andrew J. Morgan Dominic Greiffenberg Alexander Klujev Torsten Laurus Jennifer Poehlsen Ulrich Trunk Davide Mezza Bernd Schmidt Manuela Kuhn Raimund Fromme Jolanta Sztuk-Dambietz Natascha Raab Steffen Hauf Alessandro Silenzi Thomas Michelat Chen Xu Cyril Danilevski Andrea Parenti Leonce Mekinda Britta Weinhausen Grant Mills Patrik Vagovic Yoonhee Kim Henry Kirkwood Richard Bean Johan Bielecki Stephan Stern Klaus Giewekemeyer Adam R. Round Joachim Schulz Katerina Dörner Thomas D. Grant Valerio Mariani Anton Barty Adrian P. Mancuso Uwe Weierstall John C. H. Spence Henry N. Chapman Nadia Zatsepin Petra Fromme Richard A. Kirian Alexandra Ros Segmented flow generator for serial crystallography at the European X-ray free electron laser Nature Communications |
author_facet |
Austin Echelmeier Jorvani Cruz Villarreal Marc Messerschmidt Daihyun Kim Jesse D. Coe Darren Thifault Sabine Botha Ana Egatz-Gomez Sahir Gandhi Gerrit Brehm Chelsie E. Conrad Debra T. Hansen Caleb Madsen Saša Bajt J. Domingo Meza-Aguilar Dominik Oberthür Max O. Wiedorn Holger Fleckenstein Derek Mendez Juraj Knoška Jose M. Martin-Garcia Hao Hu Stella Lisova Aschkan Allahgholi Yaroslav Gevorkov Kartik Ayyer Steve Aplin Helen Mary Ginn Heinz Graafsma Andrew J. Morgan Dominic Greiffenberg Alexander Klujev Torsten Laurus Jennifer Poehlsen Ulrich Trunk Davide Mezza Bernd Schmidt Manuela Kuhn Raimund Fromme Jolanta Sztuk-Dambietz Natascha Raab Steffen Hauf Alessandro Silenzi Thomas Michelat Chen Xu Cyril Danilevski Andrea Parenti Leonce Mekinda Britta Weinhausen Grant Mills Patrik Vagovic Yoonhee Kim Henry Kirkwood Richard Bean Johan Bielecki Stephan Stern Klaus Giewekemeyer Adam R. Round Joachim Schulz Katerina Dörner Thomas D. Grant Valerio Mariani Anton Barty Adrian P. Mancuso Uwe Weierstall John C. H. Spence Henry N. Chapman Nadia Zatsepin Petra Fromme Richard A. Kirian Alexandra Ros |
author_sort |
Austin Echelmeier |
title |
Segmented flow generator for serial crystallography at the European X-ray free electron laser |
title_short |
Segmented flow generator for serial crystallography at the European X-ray free electron laser |
title_full |
Segmented flow generator for serial crystallography at the European X-ray free electron laser |
title_fullStr |
Segmented flow generator for serial crystallography at the European X-ray free electron laser |
title_full_unstemmed |
Segmented flow generator for serial crystallography at the European X-ray free electron laser |
title_sort |
segmented flow generator for serial crystallography at the european x-ray free electron laser |
publisher |
Nature Publishing Group |
series |
Nature Communications |
issn |
2041-1723 |
publishDate |
2020-09-01 |
description |
Due to the pulsed nature of X-ray free electron laser (XFEL) instruments the majority of protein crystals, which are injected using continuous jet injection techniques are wasted. Here, the authors present a microfluidic device to deliver aqueous protein crystal laden droplets segmented with an immiscible oil and demonstrate that with this device an approx. 60% reduction in sample waste was achieved for data collection of 3-deoxy-D-manno-octulosonate 8-phosphate synthase crystals at the EuXFEL. |
url |
https://doi.org/10.1038/s41467-020-18156-7 |
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doaj-813507b66a6b4fc7a68f618e55926b0f2021-09-12T11:47:29ZengNature Publishing GroupNature Communications2041-17232020-09-0111111010.1038/s41467-020-18156-7Segmented flow generator for serial crystallography at the European X-ray free electron laserAustin Echelmeier0Jorvani Cruz Villarreal1Marc Messerschmidt2Daihyun Kim3Jesse D. Coe4Darren Thifault5Sabine Botha6Ana Egatz-Gomez7Sahir Gandhi8Gerrit Brehm9Chelsie E. Conrad10Debra T. Hansen11Caleb Madsen12Saša Bajt13J. Domingo Meza-Aguilar14Dominik Oberthür15Max O. Wiedorn16Holger Fleckenstein17Derek Mendez18Juraj Knoška19Jose M. Martin-Garcia20Hao Hu21Stella Lisova22Aschkan Allahgholi23Yaroslav Gevorkov24Kartik Ayyer25Steve Aplin26Helen Mary Ginn27Heinz Graafsma28Andrew J. Morgan29Dominic Greiffenberg30Alexander Klujev31Torsten Laurus32Jennifer Poehlsen33Ulrich Trunk34Davide Mezza35Bernd Schmidt36Manuela Kuhn37Raimund Fromme38Jolanta Sztuk-Dambietz39Natascha Raab40Steffen Hauf41Alessandro Silenzi42Thomas Michelat43Chen Xu44Cyril Danilevski45Andrea Parenti46Leonce Mekinda47Britta Weinhausen48Grant Mills49Patrik Vagovic50Yoonhee Kim51Henry Kirkwood52Richard Bean53Johan Bielecki54Stephan Stern55Klaus Giewekemeyer56Adam R. Round57Joachim Schulz58Katerina Dörner59Thomas D. Grant60Valerio Mariani61Anton Barty62Adrian P. Mancuso63Uwe Weierstall64John C. H. Spence65Henry N. Chapman66Nadia Zatsepin67Petra Fromme68Richard A. Kirian69Alexandra Ros70School of Molecular Sciences, Arizona State UniversitySchool of Molecular Sciences, Arizona State UniversitySchool of Molecular Sciences, Arizona State UniversitySchool of Molecular Sciences, Arizona State UniversitySchool of Molecular Sciences, Arizona State UniversitySchool of Molecular Sciences, Arizona State UniversityCenter for Applied Structural Discovery, The Biodesign Institute, Arizona State UniversitySchool of Molecular Sciences, Arizona State UniversitySchool of Molecular Sciences, Arizona State UniversityInstitute for X-Ray Physics, University of GöttingenSchool of Molecular Sciences, Arizona State UniversityCenter for Applied Structural Discovery, The Biodesign Institute, Arizona State UniversityCenter for Applied Structural Discovery, The Biodesign Institute, Arizona State UniversityDeutsches Elektronen-SynchrotronEuropean XFELCenter for Free-Electron Laser Science, Deutsches Elektronen-SynchrotronCentre for Ultrafast Imaging, Universität HamburgCenter for Free-Electron Laser Science, Deutsches Elektronen-SynchrotronCenter for Applied Structural Discovery, The Biodesign Institute, Arizona State UniversityCenter for Free-Electron Laser Science, Deutsches Elektronen-SynchrotronSchool of Molecular Sciences, Arizona State UniversityCenter for Applied Structural Discovery, The Biodesign Institute, Arizona State UniversityCenter for Applied Structural Discovery, The Biodesign Institute, Arizona State UniversityCenter for Free-Electron Laser Science, Deutsches Elektronen-SynchrotronCenter for Free-Electron Laser Science, Deutsches Elektronen-SynchrotronCenter for Free-Electron Laser Science, Deutsches Elektronen-SynchrotronCenter for Free-Electron Laser Science, Deutsches Elektronen-SynchrotronDivision of Structural Biology, University of OxfordCenter for Free-Electron Laser Science, Deutsches Elektronen-SynchrotronCenter for Free-Electron Laser Science, Deutsches Elektronen-SynchrotronPaul Scherrer InstituteCenter for Free-Electron Laser Science, Deutsches Elektronen-SynchrotronCenter for Free-Electron Laser Science, Deutsches Elektronen-SynchrotronCenter for Free-Electron Laser Science, Deutsches Elektronen-SynchrotronCenter for Free-Electron Laser Science, Deutsches Elektronen-SynchrotronPaul Scherrer InstitutePaul Scherrer InstituteCenter for Free-Electron Laser Science, Deutsches Elektronen-SynchrotronSchool of Molecular Sciences, Arizona State UniversityEuropean XFELEuropean XFELEuropean XFELEuropean XFELEuropean XFELEuropean XFELEuropean XFELEuropean XFELEuropean XFELEuropean XFELEuropean XFELEuropean XFELEuropean XFELEuropean XFELEuropean XFELEuropean XFELEuropean XFELEuropean XFELEuropean XFELEuropean XFELEuropean XFELDepartment of Structural Biology, Jacobs School of Medicine and Biomedical Sciences, SUNY University at BuffaloCenter for Free-Electron Laser Science, Deutsches Elektronen-SynchrotronCenter for Free-Electron Laser Science, Deutsches Elektronen-SynchrotronEuropean XFELCenter for Applied Structural Discovery, The Biodesign Institute, Arizona State UniversityCenter for Applied Structural Discovery, The Biodesign Institute, Arizona State UniversityCentre for Ultrafast Imaging, Universität HamburgCenter for Applied Structural Discovery, The Biodesign Institute, Arizona State UniversitySchool of Molecular Sciences, Arizona State UniversityCenter for Applied Structural Discovery, The Biodesign Institute, Arizona State UniversitySchool of Molecular Sciences, Arizona State UniversityDue to the pulsed nature of X-ray free electron laser (XFEL) instruments the majority of protein crystals, which are injected using continuous jet injection techniques are wasted. Here, the authors present a microfluidic device to deliver aqueous protein crystal laden droplets segmented with an immiscible oil and demonstrate that with this device an approx. 60% reduction in sample waste was achieved for data collection of 3-deoxy-D-manno-octulosonate 8-phosphate synthase crystals at the EuXFEL.https://doi.org/10.1038/s41467-020-18156-7 |