Antibodies to decorin-binding protein B (DbpB) in the diagnosis of Lyme neuroborreliosis in children

• Main Authors: Heidi Sillanpää, Barbro H. Skogman, Heikki Sarvas, Ilkka J.T. Seppälä, Pekka Lahdenne
• Format: Article
• Language: English
• Published: Elsevier 2014-11-01
• Series: International Journal of Infectious Diseases
• Subjects: Lyme neuroborreliosis; DbpB; Children; Borrelia burgdorferi sensu lato; CSF
• Online Access: http://www.sciencedirect.com/science/article/pii/S1201971214015902
• ISSN: 1201-9712; 1878-3511

Background: Laboratory support is needed to confirm the clinical diagnosis of Lyme neuroborreliosis (LNB). Antibodies to Borrelia-specific proteins have been used to improve serological diagnostics. The aims of this study were to assess the occurrence of antibodies to decorin-binding protein B (DbpB) in serum and cerebrospinal fluid (CSF) in children with LNB and to evaluate the performance of DbpB variants in the diagnosis of LNB in children. Methods: Serum and CSF sample pairs were available from 57 children evaluated for LNB. Based on the presence of anti-flagella antibodies and pleocytosis in the CSF, patients were divided into three different groups: confirmed LNB (n = 24), possible LNB (n = 16), and non LNB (n = 17). Recombinant DbpBs from three Borrelia burgdorferi sensu lato species – Borrelia burgdorferi sensu stricto, Borrelia garinii, and Borrelia afzelii – were used in an ELISA to detect IgG antibodies. Results: The sensitivity of variant recombinant DbpBs in serum and CSF samples varied between 0% and 46% and between 0% and 42%, respectively. In CSF, the most sensitive antigen was the DbpB variant from B. garinii. Conclusions: Serum or CSF antibodies to DbpB do not appear to be beneficial in the laboratory diagnosis of LNB in children.