Prevalence of Ciprofloxacin Resistance Among Gram-Negative Bacilli in a Tertiary Care Hospital
Background: Bacterial resistance to antibiotics is a growing therapeutic problem, both in the community and the hospitals, which involves all the antibiotics including fluoroquinolones. The decreased susceptibility to fluoroquinolones arises mainly due to single-step mutations in the gyrA and th...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
JCDR Research and Publications Private Limited
2012-04-01
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Series: | Journal of Clinical and Diagnostic Research |
Subjects: | |
Online Access: | https://jcdr.net/articles/PDF/1900/8-%203490.A..pdf |
Summary: | Background: Bacterial resistance to antibiotics is a growing
therapeutic problem, both in the community and the hospitals,
which involves all the antibiotics including fluoroquinolones. The
decreased susceptibility to fluoroquinolones arises mainly due
to single-step mutations in the gyrA and the parC genes, which
encode the fluoroquinolones targets, the topoisomerase enzymes,
thus conferring cross-resistance to the fluoroquinolones. In
1998, some mobile elements with a potential for the horizontal
transfer of the quinolone resistance genes were described. The
loci which are responsible for this plasmid-mediated quinolone
resistance, which have been designated as qnr A, qnr B and qnr
S, have been identified in the Enterobacteriaceae species.
Aim: This study was undertaken to evaluate the susceptibility
pattern of the isolates to various antibiotics and to know the
prevalence rate of ciprofloxacin resistance in our hospital.
Materials & Methods: A total of 734 gram-negative bacilli (GNB)
which were isolated from various clinical samples over a period
of six months, were subjected to antibiotic susceptibility testing.
Isolates with resistance or with a decreased susceptibility to
ciprofloxacin (≤20 mm) were then screened for their minimum
inhibitory concentration (MIC) by using the E-test.
Results: Out of 734 GNB, 235 (32%) isolates were resistant
to ciprofloxacin. The MIC of these isolates ranged from 4 to >
32µg/ml.
Conclusion: The resistance rate to ciprofloxacin was 32% in
our study. Most of the ciprofloxacin resistant isolates were from
urinary tract infections (UTI). The ciprofloxacin resistance was
also closely associated with multi-drug resistance, thus limiting
the treatment options. Ciprofloxacin resistance can be used as
a general surrogate marker of multidrug resistance, thus limiting
the already restricted treatment options. The considerably high
MIC values for ciprofloxacin in this study reflected the extent of
the treatment problems for these resistant isolates and a need
for the continuous evaluation of the commonly used antibiotics. |
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ISSN: | 2249-782X 0973-709X |