SATB1 overexpression regulates the development and progression in bladder cancer through EMT.
The global gene regulator Special AT-rich sequence-binding protein-1 (SATB1) has been reported to induce EMT-like changes and be associated with poor clinical outcome in several cancers. This study aims to evaluate whether SATB1 affects the biological behaviors of bladder transitional cell carcinoma...
Main Authors: | , , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4338074?pdf=render |
id |
doaj-8189bb85488c44358b5bb3d783580b22 |
---|---|
record_format |
Article |
spelling |
doaj-8189bb85488c44358b5bb3d783580b222020-11-25T02:33:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011751810.1371/journal.pone.0117518SATB1 overexpression regulates the development and progression in bladder cancer through EMT.Feng WanChao ChengZongwei WangXingyuan XiaoHanqing ZengShian XingXuepan ChenJin WangSen LiYoupeng ZhangWei XiangZhineng ZhuCameron JohnsonZhaohui ZhuThe global gene regulator Special AT-rich sequence-binding protein-1 (SATB1) has been reported to induce EMT-like changes and be associated with poor clinical outcome in several cancers. This study aims to evaluate whether SATB1 affects the biological behaviors of bladder transitional cell carcinoma (BTCC) and further elucidate if this effect works through an epithelial-mesenchymal transition (EMT) pathway. The expression of SATB1, E-cadherin (epithelial markers), vimentin (mesenchymal markers) in BTCC tissues and adjacent noncancerous tissues, as well as in two cell lines of bladder cancer were investigated. Whether the SATB1 expression is associated with clinicopathological factors or not was statistically analyzed. Cell invasion and migration, cell cycle, cell proliferation and apoptosis were evaluated in SATB1 knockdown and overexpressed cell lines. Our results showed that the expression of SATB1 was remarkably up-regulated both in BTCC tissues and in bladder cancer cell lines with high potential of metastasis. The results were also associated with EMT markers and poor prognosis of BTCC patients. Moreover, SATB1 induced EMT processes through downregulation of E-cadherin, upregulation of E-cadherin repressors (Snail, Slug and vimentin). SATB1 also promoted cell cycle progression, cell proliferation, cell invasion and cell migration, but did not alter cell survival. In conclusion, our results suggest that SATB1 plays a crucial role in the progression of bladder cancer by regulating genes controlling EMT processes. Further, it may be a novel therapeutic target for aggressive bladder cancers.http://europepmc.org/articles/PMC4338074?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Feng Wan Chao Cheng Zongwei Wang Xingyuan Xiao Hanqing Zeng Shian Xing Xuepan Chen Jin Wang Sen Li Youpeng Zhang Wei Xiang Zhineng Zhu Cameron Johnson Zhaohui Zhu |
spellingShingle |
Feng Wan Chao Cheng Zongwei Wang Xingyuan Xiao Hanqing Zeng Shian Xing Xuepan Chen Jin Wang Sen Li Youpeng Zhang Wei Xiang Zhineng Zhu Cameron Johnson Zhaohui Zhu SATB1 overexpression regulates the development and progression in bladder cancer through EMT. PLoS ONE |
author_facet |
Feng Wan Chao Cheng Zongwei Wang Xingyuan Xiao Hanqing Zeng Shian Xing Xuepan Chen Jin Wang Sen Li Youpeng Zhang Wei Xiang Zhineng Zhu Cameron Johnson Zhaohui Zhu |
author_sort |
Feng Wan |
title |
SATB1 overexpression regulates the development and progression in bladder cancer through EMT. |
title_short |
SATB1 overexpression regulates the development and progression in bladder cancer through EMT. |
title_full |
SATB1 overexpression regulates the development and progression in bladder cancer through EMT. |
title_fullStr |
SATB1 overexpression regulates the development and progression in bladder cancer through EMT. |
title_full_unstemmed |
SATB1 overexpression regulates the development and progression in bladder cancer through EMT. |
title_sort |
satb1 overexpression regulates the development and progression in bladder cancer through emt. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
The global gene regulator Special AT-rich sequence-binding protein-1 (SATB1) has been reported to induce EMT-like changes and be associated with poor clinical outcome in several cancers. This study aims to evaluate whether SATB1 affects the biological behaviors of bladder transitional cell carcinoma (BTCC) and further elucidate if this effect works through an epithelial-mesenchymal transition (EMT) pathway. The expression of SATB1, E-cadherin (epithelial markers), vimentin (mesenchymal markers) in BTCC tissues and adjacent noncancerous tissues, as well as in two cell lines of bladder cancer were investigated. Whether the SATB1 expression is associated with clinicopathological factors or not was statistically analyzed. Cell invasion and migration, cell cycle, cell proliferation and apoptosis were evaluated in SATB1 knockdown and overexpressed cell lines. Our results showed that the expression of SATB1 was remarkably up-regulated both in BTCC tissues and in bladder cancer cell lines with high potential of metastasis. The results were also associated with EMT markers and poor prognosis of BTCC patients. Moreover, SATB1 induced EMT processes through downregulation of E-cadherin, upregulation of E-cadherin repressors (Snail, Slug and vimentin). SATB1 also promoted cell cycle progression, cell proliferation, cell invasion and cell migration, but did not alter cell survival. In conclusion, our results suggest that SATB1 plays a crucial role in the progression of bladder cancer by regulating genes controlling EMT processes. Further, it may be a novel therapeutic target for aggressive bladder cancers. |
url |
http://europepmc.org/articles/PMC4338074?pdf=render |
work_keys_str_mv |
AT fengwan satb1overexpressionregulatesthedevelopmentandprogressioninbladdercancerthroughemt AT chaocheng satb1overexpressionregulatesthedevelopmentandprogressioninbladdercancerthroughemt AT zongweiwang satb1overexpressionregulatesthedevelopmentandprogressioninbladdercancerthroughemt AT xingyuanxiao satb1overexpressionregulatesthedevelopmentandprogressioninbladdercancerthroughemt AT hanqingzeng satb1overexpressionregulatesthedevelopmentandprogressioninbladdercancerthroughemt AT shianxing satb1overexpressionregulatesthedevelopmentandprogressioninbladdercancerthroughemt AT xuepanchen satb1overexpressionregulatesthedevelopmentandprogressioninbladdercancerthroughemt AT jinwang satb1overexpressionregulatesthedevelopmentandprogressioninbladdercancerthroughemt AT senli satb1overexpressionregulatesthedevelopmentandprogressioninbladdercancerthroughemt AT youpengzhang satb1overexpressionregulatesthedevelopmentandprogressioninbladdercancerthroughemt AT weixiang satb1overexpressionregulatesthedevelopmentandprogressioninbladdercancerthroughemt AT zhinengzhu satb1overexpressionregulatesthedevelopmentandprogressioninbladdercancerthroughemt AT cameronjohnson satb1overexpressionregulatesthedevelopmentandprogressioninbladdercancerthroughemt AT zhaohuizhu satb1overexpressionregulatesthedevelopmentandprogressioninbladdercancerthroughemt |
_version_ |
1724813075963445248 |