Biological controls for standardization and interpretation of adaptive immune receptor repertoire profiling

• Main Authors: Johannes Trück, Anne Eugster, Pierre Barennes, Christopher M Tipton, Eline T Luning Prak, Davide Bagnara, Cinque Soto, Jacob S Sherkow, Aimee S Payne, Marie-Paule Lefranc, Andrew Farmer, The AIRR Community, Magnolia Bostick, Encarnita Mariotti-Ferrandiz
• Format: Article
• Language: English
• Published: eLife Sciences Publications Ltd 2021-05-01
• Series: eLife
• Subjects: T-cell Receptor (TCR); B-cell Receptor (BCR); next generation sequencing (NGS); immunoglobulin; antibody; IG
• Online Access: https://elifesciences.org/articles/66274

Use of adaptive immune receptor repertoire sequencing (AIRR-seq) has become widespread, providing new insights into the immune system with potential broad clinical and diagnostic applications. However, like many high-throughput technologies, it comes with several problems, and the AIRR Community was established to understand and help solve them. We, the AIRR Community’s Biological Resources Working Group, have surveyed scientists about the need for standards and controls in generating and annotating AIRR-seq data. Here, we review the current status of AIRR-seq, provide the results of our survey, and based on them, offer recommendations for developing AIRR-seq standards and controls, including future work.