Infiltrating CD16+ Are Associated with a Reduction in Peripheral CD14+CD16++ Monocytes and Severe Forms of Lupus Nephritis
Our aim was to characterize glomerular monocytes (Mo) infiltration and to correlate them with peripheral circulating Mo subsets and severity of lupus nephritis (LN). Methods. We evaluated 48 LN biopsy samples from a referral hospital. Recognition of Mo cells was done using microscopic view and immun...
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doaj-81a548f88f494577b10e727493b4aa712020-11-25T02:19:09ZengHindawi LimitedAutoimmune Diseases2090-04222090-04302016-01-01201610.1155/2016/93243159324315Infiltrating CD16+ Are Associated with a Reduction in Peripheral CD14+CD16++ Monocytes and Severe Forms of Lupus NephritisAnabel Barrera García0José A. Gómez-Puerta1Luis F. Arias2Catalina Burbano3Mauricio Restrepo4Adriana L. Vanegas5Carlos H. Muñoz6Mauricio Rojas7Luis A. González8Gloria Vásquez9Grupo de Inmunología Celular e Inmunogenética, GICIG, Universidad de Antioquía, Medellín, ColombiaGrupo de Inmunología Celular e Inmunogenética, GICIG, Universidad de Antioquía, Medellín, ColombiaGrupo de Patología Renal y Trasplante, Universidad de Antioquía, Medellín, ColombiaGrupo de Inmunología Celular e Inmunogenética, GICIG, Universidad de Antioquía, Medellín, ColombiaGrupo de Reumatología, Universidad de Antioquía, Medellín, ColombiaGrupo de Reumatología, Universidad de Antioquía, Medellín, ColombiaGrupo de Reumatología, Universidad de Antioquía, Medellín, ColombiaGrupo de Inmunología Celular e Inmunogenética, GICIG, Universidad de Antioquía, Medellín, ColombiaGrupo de Reumatología, Universidad de Antioquía, Medellín, ColombiaGrupo de Inmunología Celular e Inmunogenética, GICIG, Universidad de Antioquía, Medellín, ColombiaOur aim was to characterize glomerular monocytes (Mo) infiltration and to correlate them with peripheral circulating Mo subsets and severity of lupus nephritis (LN). Methods. We evaluated 48 LN biopsy samples from a referral hospital. Recognition of Mo cells was done using microscopic view and immunohistochemistry stain with CD14 and CD16. Based on the number of cells, we classified LN samples as low degree of diffuse infiltration (<5 cells) and high degree of diffuse infiltration (≥5 cells). Immunophenotyping of peripheral Mo subsets was done using flow cytometry. Results. Mean age was 34.0±11.7 years and the mean SLEDAI was 17.5±6.9. The most common SLE manifestations were proteinuria (91%) and hypocomplementemia (75%). Severe LN was found in 70% of patients (Class III, 27%; Class IV, 43%). Severe LN patients and patients with higher grade of CD16+ infiltration had lower levels of nonclassical (CD14+CD16++) Mo in peripheral blood. Conclusions. Our results might suggest that those patients with more severe forms of LN had a higher grade of CD14+CD16+ infiltration and lower peripheral levels of nonclassical (CD14+CD16++) Mo and might reflect a recruitment process in renal tissues. However, given the small sample, our results must be interpreted carefully.http://dx.doi.org/10.1155/2016/9324315 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anabel Barrera García José A. Gómez-Puerta Luis F. Arias Catalina Burbano Mauricio Restrepo Adriana L. Vanegas Carlos H. Muñoz Mauricio Rojas Luis A. González Gloria Vásquez |
spellingShingle |
Anabel Barrera García José A. Gómez-Puerta Luis F. Arias Catalina Burbano Mauricio Restrepo Adriana L. Vanegas Carlos H. Muñoz Mauricio Rojas Luis A. González Gloria Vásquez Infiltrating CD16+ Are Associated with a Reduction in Peripheral CD14+CD16++ Monocytes and Severe Forms of Lupus Nephritis Autoimmune Diseases |
author_facet |
Anabel Barrera García José A. Gómez-Puerta Luis F. Arias Catalina Burbano Mauricio Restrepo Adriana L. Vanegas Carlos H. Muñoz Mauricio Rojas Luis A. González Gloria Vásquez |
author_sort |
Anabel Barrera García |
title |
Infiltrating CD16+ Are Associated with a Reduction in Peripheral CD14+CD16++ Monocytes and Severe Forms of Lupus Nephritis |
title_short |
Infiltrating CD16+ Are Associated with a Reduction in Peripheral CD14+CD16++ Monocytes and Severe Forms of Lupus Nephritis |
title_full |
Infiltrating CD16+ Are Associated with a Reduction in Peripheral CD14+CD16++ Monocytes and Severe Forms of Lupus Nephritis |
title_fullStr |
Infiltrating CD16+ Are Associated with a Reduction in Peripheral CD14+CD16++ Monocytes and Severe Forms of Lupus Nephritis |
title_full_unstemmed |
Infiltrating CD16+ Are Associated with a Reduction in Peripheral CD14+CD16++ Monocytes and Severe Forms of Lupus Nephritis |
title_sort |
infiltrating cd16+ are associated with a reduction in peripheral cd14+cd16++ monocytes and severe forms of lupus nephritis |
publisher |
Hindawi Limited |
series |
Autoimmune Diseases |
issn |
2090-0422 2090-0430 |
publishDate |
2016-01-01 |
description |
Our aim was to characterize glomerular monocytes (Mo) infiltration and to correlate them with peripheral circulating Mo subsets and severity of lupus nephritis (LN). Methods. We evaluated 48 LN biopsy samples from a referral hospital. Recognition of Mo cells was done using microscopic view and immunohistochemistry stain with CD14 and CD16. Based on the number of cells, we classified LN samples as low degree of diffuse infiltration (<5 cells) and high degree of diffuse infiltration (≥5 cells). Immunophenotyping of peripheral Mo subsets was done using flow cytometry. Results. Mean age was 34.0±11.7 years and the mean SLEDAI was 17.5±6.9. The most common SLE manifestations were proteinuria (91%) and hypocomplementemia (75%). Severe LN was found in 70% of patients (Class III, 27%; Class IV, 43%). Severe LN patients and patients with higher grade of CD16+ infiltration had lower levels of nonclassical (CD14+CD16++) Mo in peripheral blood. Conclusions. Our results might suggest that those patients with more severe forms of LN had a higher grade of CD14+CD16+ infiltration and lower peripheral levels of nonclassical (CD14+CD16++) Mo and might reflect a recruitment process in renal tissues. However, given the small sample, our results must be interpreted carefully. |
url |
http://dx.doi.org/10.1155/2016/9324315 |
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