Evidence That ADAM17 Mediates the Protective Action of CGRP against Angiotensin II-Induced Inflammation in Vascular Smooth Muscle Cells
Calcitonin gene-related peptide (CGRP) has a potent protective action on the cardiovascular system; however, little is known about the role of CGRP in angiotensin II- (Ang II-) induced inflammation of vascular smooth muscle cells (VSMCs). This study is aimed at determining the anti-inflammatory effe...
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Series: | Mediators of Inflammation |
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doaj-81b9c53d04154306b4f62dab5901b7e32020-11-25T00:54:28ZengHindawi LimitedMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/21093522109352Evidence That ADAM17 Mediates the Protective Action of CGRP against Angiotensin II-Induced Inflammation in Vascular Smooth Muscle CellsSi-yu Zeng0Li Yang1Chen-liang Hong2Hui-qin Lu3Qiu-jiang Yan4Yan Chen5Xu-ping Qin6Institution of Drug Clinical Trial, Guangdong Second Provincial General Hospital, Guangzhou 510317, ChinaLaboratory of Vascular Biology, Institute of Pharmacy and Pharmacology, University of South China, Hengyang, 421001 Hunan, ChinaLaboratory of Vascular Biology, Institute of Pharmacy and Pharmacology, University of South China, Hengyang, 421001 Hunan, ChinaInstitution of Drug Clinical Trial, Guangdong Second Provincial General Hospital, Guangzhou 510317, ChinaDepartment of Cardiac & Thoracic Surgery, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510000, ChinaInstitution of Drug Clinical Trial, Guangdong Second Provincial General Hospital, Guangzhou 510317, ChinaLaboratory of Vascular Biology, Institute of Pharmacy and Pharmacology, University of South China, Hengyang, 421001 Hunan, ChinaCalcitonin gene-related peptide (CGRP) has a potent protective action on the cardiovascular system; however, little is known about the role of CGRP in angiotensin II- (Ang II-) induced inflammation of vascular smooth muscle cells (VSMCs). This study is aimed at determining the anti-inflammatory effect of CGRP in Ang II-treated VSMCs and whether a disintegrin and metalloproteinase 17 (ADAM17) modulates this protective action. Small interference RNA (siRNA) and inhibitors of CGRP, epidermal growth factor receptor (EGFR), and extracellular signal-regulated kinase 1/2 (ERK1/2) were adopted to investigate their effect on Ang II-induced inflammation in VSMCs. Here, we found that CGRP could inhibit inflammation and decrease ADAM17 expression and activation of EGFR and ERK1/2 in VSMCs stimulated with Ang II. Results of siRNA demonstrated that ADAM17 siRNA attenuated Ang II-induced inflammation and up-regulation of activities of EGFR and ERK1/2 in VSMCs. Furthermore, the EGFR-ERK1/2 pathway promoted Ang II-induced VSMC inflammation. In summary, these findings identify the anti-inflammatory effect of CGRP in VSMCs stimulated by Ang II and suggest that ADAM17 is involved in the protective effect of CGRP against Ang II-induced inflammation via the EGFR-ERK1/2 pathway in VSMCs.http://dx.doi.org/10.1155/2018/2109352 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Si-yu Zeng Li Yang Chen-liang Hong Hui-qin Lu Qiu-jiang Yan Yan Chen Xu-ping Qin |
spellingShingle |
Si-yu Zeng Li Yang Chen-liang Hong Hui-qin Lu Qiu-jiang Yan Yan Chen Xu-ping Qin Evidence That ADAM17 Mediates the Protective Action of CGRP against Angiotensin II-Induced Inflammation in Vascular Smooth Muscle Cells Mediators of Inflammation |
author_facet |
Si-yu Zeng Li Yang Chen-liang Hong Hui-qin Lu Qiu-jiang Yan Yan Chen Xu-ping Qin |
author_sort |
Si-yu Zeng |
title |
Evidence That ADAM17 Mediates the Protective Action of CGRP against Angiotensin II-Induced Inflammation in Vascular Smooth Muscle Cells |
title_short |
Evidence That ADAM17 Mediates the Protective Action of CGRP against Angiotensin II-Induced Inflammation in Vascular Smooth Muscle Cells |
title_full |
Evidence That ADAM17 Mediates the Protective Action of CGRP against Angiotensin II-Induced Inflammation in Vascular Smooth Muscle Cells |
title_fullStr |
Evidence That ADAM17 Mediates the Protective Action of CGRP against Angiotensin II-Induced Inflammation in Vascular Smooth Muscle Cells |
title_full_unstemmed |
Evidence That ADAM17 Mediates the Protective Action of CGRP against Angiotensin II-Induced Inflammation in Vascular Smooth Muscle Cells |
title_sort |
evidence that adam17 mediates the protective action of cgrp against angiotensin ii-induced inflammation in vascular smooth muscle cells |
publisher |
Hindawi Limited |
series |
Mediators of Inflammation |
issn |
0962-9351 1466-1861 |
publishDate |
2018-01-01 |
description |
Calcitonin gene-related peptide (CGRP) has a potent protective action on the cardiovascular system; however, little is known about the role of CGRP in angiotensin II- (Ang II-) induced inflammation of vascular smooth muscle cells (VSMCs). This study is aimed at determining the anti-inflammatory effect of CGRP in Ang II-treated VSMCs and whether a disintegrin and metalloproteinase 17 (ADAM17) modulates this protective action. Small interference RNA (siRNA) and inhibitors of CGRP, epidermal growth factor receptor (EGFR), and extracellular signal-regulated kinase 1/2 (ERK1/2) were adopted to investigate their effect on Ang II-induced inflammation in VSMCs. Here, we found that CGRP could inhibit inflammation and decrease ADAM17 expression and activation of EGFR and ERK1/2 in VSMCs stimulated with Ang II. Results of siRNA demonstrated that ADAM17 siRNA attenuated Ang II-induced inflammation and up-regulation of activities of EGFR and ERK1/2 in VSMCs. Furthermore, the EGFR-ERK1/2 pathway promoted Ang II-induced VSMC inflammation. In summary, these findings identify the anti-inflammatory effect of CGRP in VSMCs stimulated by Ang II and suggest that ADAM17 is involved in the protective effect of CGRP against Ang II-induced inflammation via the EGFR-ERK1/2 pathway in VSMCs. |
url |
http://dx.doi.org/10.1155/2018/2109352 |
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