Altered Esophageal Mucosal Structure in Patients with Celiac Disease
Background/Aim. Reflux symptoms (RS) are common in patients with celiac disease (CD), a chronic enteropathy that affects primarily the small intestine. We evaluated mucosal integrity and motility of the lower esophagus as mechanisms contributing to RS generation in patients with CD. Methods. We enro...
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doaj-81d7bfa499a64b7089ee5e95f8cb10392020-11-24T23:21:49ZengHindawi LimitedCanadian Journal of Gastroenterology and Hepatology2291-27892291-27972016-01-01201610.1155/2016/19806861980686Altered Esophageal Mucosal Structure in Patients with Celiac DiseaseMaría Inés Pinto-Sánchez0Fabio D. Nachman1Claudia Fuxman2Guido Iantorno3Hui Jer Hwang4Andrés Ditaranto5Florencia Costa6Gabriela Longarini7Xuan Yu Wang8Xianxi Huang9Horacio Vázquez10María L. Moreno11Sonia Niveloni12Premysl Bercik13Edgardo Smecuol14Roberto Mazure15Claudio Bilder16Eduardo C. Mauriño17Elena F. Verdu18Julio C. Bai19Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, L8S 4K1, CanadaDepartment of Medicine, “Dr. Carlos Bonorino Udaondo” Gastroenterology Hospital, 1264 Buenos Aires, ArgentinaFavaloro University Hospital, 1093 Buenos Aires, ArgentinaDepartment of Medicine, “Dr. Carlos Bonorino Udaondo” Gastroenterology Hospital, 1264 Buenos Aires, ArgentinaDepartment of Medicine, “Dr. Carlos Bonorino Udaondo” Gastroenterology Hospital, 1264 Buenos Aires, ArgentinaFavaloro University Hospital, 1093 Buenos Aires, ArgentinaDepartment of Medicine, “Dr. Carlos Bonorino Udaondo” Gastroenterology Hospital, 1264 Buenos Aires, ArgentinaDepartment of Medicine, “Dr. Carlos Bonorino Udaondo” Gastroenterology Hospital, 1264 Buenos Aires, ArgentinaFarncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, L8S 4K1, CanadaFarncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, L8S 4K1, CanadaDepartment of Medicine, “Dr. Carlos Bonorino Udaondo” Gastroenterology Hospital, 1264 Buenos Aires, ArgentinaDepartment of Medicine, “Dr. Carlos Bonorino Udaondo” Gastroenterology Hospital, 1264 Buenos Aires, ArgentinaDepartment of Medicine, “Dr. Carlos Bonorino Udaondo” Gastroenterology Hospital, 1264 Buenos Aires, ArgentinaFarncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, L8S 4K1, CanadaDepartment of Medicine, “Dr. Carlos Bonorino Udaondo” Gastroenterology Hospital, 1264 Buenos Aires, ArgentinaDepartment of Medicine, “Dr. Carlos Bonorino Udaondo” Gastroenterology Hospital, 1264 Buenos Aires, ArgentinaFavaloro University Hospital, 1093 Buenos Aires, ArgentinaDepartment of Medicine, “Dr. Carlos Bonorino Udaondo” Gastroenterology Hospital, 1264 Buenos Aires, ArgentinaFarncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON, L8S 4K1, CanadaDepartment of Medicine, “Dr. Carlos Bonorino Udaondo” Gastroenterology Hospital, 1264 Buenos Aires, ArgentinaBackground/Aim. Reflux symptoms (RS) are common in patients with celiac disease (CD), a chronic enteropathy that affects primarily the small intestine. We evaluated mucosal integrity and motility of the lower esophagus as mechanisms contributing to RS generation in patients with CD. Methods. We enrolled newly diagnosed CD patients with and without RS, nonceliac patients with classical reflux disease (GERD), and controls (without RS). Endoscopic biopsies from the distal esophagus were assessed for dilated intercellular space (DIS) by light microscopy and electron microscopy. Tight junction (TJ) mRNA proteins expression for zonula occludens-1 (ZO-1) and claudin-2 and claudin-3 (CLDN-2; CLDN-3) was determined using qRT-PCR. Results. DIS scores were higher in patients with active CD than in controls, but similar to GERD patients. The altered DIS was found even in CD patients without RS and normalized after one year of a gluten-free diet. CD patients with and without RS had lower expression of ZO-1 than controls. The expression of CLDN-2 and CLDN-3 was similar in CD and GERD patients. Conclusions. Our study shows that patients with active CD have altered esophageal mucosal integrity, independently of the presence of RS. The altered expression of ZO-1 may underlie loss of TJ integrity in the esophageal mucosa and may contribute to RS generation.http://dx.doi.org/10.1155/2016/1980686 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
María Inés Pinto-Sánchez Fabio D. Nachman Claudia Fuxman Guido Iantorno Hui Jer Hwang Andrés Ditaranto Florencia Costa Gabriela Longarini Xuan Yu Wang Xianxi Huang Horacio Vázquez María L. Moreno Sonia Niveloni Premysl Bercik Edgardo Smecuol Roberto Mazure Claudio Bilder Eduardo C. Mauriño Elena F. Verdu Julio C. Bai |
spellingShingle |
María Inés Pinto-Sánchez Fabio D. Nachman Claudia Fuxman Guido Iantorno Hui Jer Hwang Andrés Ditaranto Florencia Costa Gabriela Longarini Xuan Yu Wang Xianxi Huang Horacio Vázquez María L. Moreno Sonia Niveloni Premysl Bercik Edgardo Smecuol Roberto Mazure Claudio Bilder Eduardo C. Mauriño Elena F. Verdu Julio C. Bai Altered Esophageal Mucosal Structure in Patients with Celiac Disease Canadian Journal of Gastroenterology and Hepatology |
author_facet |
María Inés Pinto-Sánchez Fabio D. Nachman Claudia Fuxman Guido Iantorno Hui Jer Hwang Andrés Ditaranto Florencia Costa Gabriela Longarini Xuan Yu Wang Xianxi Huang Horacio Vázquez María L. Moreno Sonia Niveloni Premysl Bercik Edgardo Smecuol Roberto Mazure Claudio Bilder Eduardo C. Mauriño Elena F. Verdu Julio C. Bai |
author_sort |
María Inés Pinto-Sánchez |
title |
Altered Esophageal Mucosal Structure in Patients with Celiac Disease |
title_short |
Altered Esophageal Mucosal Structure in Patients with Celiac Disease |
title_full |
Altered Esophageal Mucosal Structure in Patients with Celiac Disease |
title_fullStr |
Altered Esophageal Mucosal Structure in Patients with Celiac Disease |
title_full_unstemmed |
Altered Esophageal Mucosal Structure in Patients with Celiac Disease |
title_sort |
altered esophageal mucosal structure in patients with celiac disease |
publisher |
Hindawi Limited |
series |
Canadian Journal of Gastroenterology and Hepatology |
issn |
2291-2789 2291-2797 |
publishDate |
2016-01-01 |
description |
Background/Aim. Reflux symptoms (RS) are common in patients with celiac disease (CD), a chronic enteropathy that affects primarily the small intestine. We evaluated mucosal integrity and motility of the lower esophagus as mechanisms contributing to RS generation in patients with CD. Methods. We enrolled newly diagnosed CD patients with and without RS, nonceliac patients with classical reflux disease (GERD), and controls (without RS). Endoscopic biopsies from the distal esophagus were assessed for dilated intercellular space (DIS) by light microscopy and electron microscopy. Tight junction (TJ) mRNA proteins expression for zonula occludens-1 (ZO-1) and claudin-2 and claudin-3 (CLDN-2; CLDN-3) was determined using qRT-PCR. Results. DIS scores were higher in patients with active CD than in controls, but similar to GERD patients. The altered DIS was found even in CD patients without RS and normalized after one year of a gluten-free diet. CD patients with and without RS had lower expression of ZO-1 than controls. The expression of CLDN-2 and CLDN-3 was similar in CD and GERD patients. Conclusions. Our study shows that patients with active CD have altered esophageal mucosal integrity, independently of the presence of RS. The altered expression of ZO-1 may underlie loss of TJ integrity in the esophageal mucosa and may contribute to RS generation. |
url |
http://dx.doi.org/10.1155/2016/1980686 |
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