Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell
<p>Abstract</p> <p>Background</p> <p>Current methods for accurate quantification of nucleic acids typically begin with a template preparation step in which DNA and/or RNA are freed of bound proteins and are then purified. Isolation of RNA is particularly challenging bec...
Main Authors: | Anshelevich Aleksandra, Hartshorn Cristina, Wangh Lawrence J |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2005-01-01
|
Series: | BMC Biotechnology |
Online Access: | http://www.biomedcentral.com/1472-6750/5/2 |
Similar Items
-
Single-cell duplex RT-LATE-PCR reveals <it>Oct4 </it>and <it>Xist </it>RNA gradients in 8-cell embryos
by: Hartung Odelya, et al.
Published: (2007-12-01) -
Single tube single-color analysis of rifampicin and isoniazid allelic variants in MDR-TB
by: L. Wangh, et al.
Published: (2014-04-01) -
One-Tube Nested Real-Time PCR Assay for Rapid Screening of Porcine Cytomegalovirus in Clinical Samples
by: Hye-young Wang, et al.
Published: (2020-10-01) -
Optimized protocol for the extraction of RNA and DNA from frozen whole blood sample stored in a single EDTA tube
by: Hirotaka Yamagata, et al.
Published: (2021-08-01) -
An asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy
by: Bi Yanzhen, et al.
Published: (2012-07-01)