To evaluate the role of p16ink4a immunocytochemistry for detection of cin2+ in women detected screen positive by visual inspection using acetic acid
Objective: To evaluate the role of p16Ink4a immunostaining for the detection of cervical intraepithelial neoplasia (CIN2+) in women who had a positive screening test using visual inspection with acetic acid (VIA). Methods: Opportunistic screening of women (30–50 years) coming to the gynecology clini...
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Wolters Kluwer Medknow Publications
2020-01-01
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doaj-81eca1e047574f86b8f69f769ac7d7b82020-11-25T02:11:55ZengWolters Kluwer Medknow PublicationsJournal of Cytology0970-93712020-01-01372828610.4103/JOC.JOC_89_18To evaluate the role of p16ink4a immunocytochemistry for detection of cin2+ in women detected screen positive by visual inspection using acetic acidLalita VermaSaritha ShamsunderSunita MalikRashmi AroraObjective: To evaluate the role of p16Ink4a immunostaining for the detection of cervical intraepithelial neoplasia (CIN2+) in women who had a positive screening test using visual inspection with acetic acid (VIA). Methods: Opportunistic screening of women (30–50 years) coming to the gynecology clinic by VIA was performed; the screen-positive women were included in the study which had the institutional review board (IRB) approval. A cytology slide for p16Ink4a immunostaining, colposcopy, and biopsy was then performed sequentially. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of p16Ink4a immunocytochemistry were evaluated with histopathology as the gold standard. Results: p16Ink4a positivity showed a linear correlation with the increasing grade of CIN. p16Ink4a positivity was seen in 6% of CIN 1, 80% of CIN 2, 100% of CIN 3, and squamous cell carcinoma. The sensitivity and specificity of p16Ink4a immunocytochemistry for detecting CIN 2 or more was 87.5% (95%CI 61.65–98.45) and 97.06% (95%CI 84.67–99.93). Colposcopy had an equal sensitivity of 87.5% (95% CI 61.65–98.45) and specificity of 50% (95% CI 32.43–67.57), respectively. Conclusion: With high sensitivity and specificity, p16Ink4a immunocytochemistry could be a viable option for triaging VIA-positive women.http://www.jcytol.org/article.asp?issn=0970-9371;year=2020;volume=37;issue=2;spage=82;epage=86;aulast=Vermacervical intraepithelial neoplasiacolposcopyhuman papillomavirusp16ink4a immunocytochemistryvisual inspection with acetic acid |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lalita Verma Saritha Shamsunder Sunita Malik Rashmi Arora |
spellingShingle |
Lalita Verma Saritha Shamsunder Sunita Malik Rashmi Arora To evaluate the role of p16ink4a immunocytochemistry for detection of cin2+ in women detected screen positive by visual inspection using acetic acid Journal of Cytology cervical intraepithelial neoplasia colposcopy human papillomavirus p16ink4a immunocytochemistry visual inspection with acetic acid |
author_facet |
Lalita Verma Saritha Shamsunder Sunita Malik Rashmi Arora |
author_sort |
Lalita Verma |
title |
To evaluate the role of p16ink4a immunocytochemistry for detection of cin2+ in women detected screen positive by visual inspection using acetic acid |
title_short |
To evaluate the role of p16ink4a immunocytochemistry for detection of cin2+ in women detected screen positive by visual inspection using acetic acid |
title_full |
To evaluate the role of p16ink4a immunocytochemistry for detection of cin2+ in women detected screen positive by visual inspection using acetic acid |
title_fullStr |
To evaluate the role of p16ink4a immunocytochemistry for detection of cin2+ in women detected screen positive by visual inspection using acetic acid |
title_full_unstemmed |
To evaluate the role of p16ink4a immunocytochemistry for detection of cin2+ in women detected screen positive by visual inspection using acetic acid |
title_sort |
to evaluate the role of p16ink4a immunocytochemistry for detection of cin2+ in women detected screen positive by visual inspection using acetic acid |
publisher |
Wolters Kluwer Medknow Publications |
series |
Journal of Cytology |
issn |
0970-9371 |
publishDate |
2020-01-01 |
description |
Objective: To evaluate the role of p16Ink4a immunostaining for the detection of cervical intraepithelial neoplasia (CIN2+) in women who had a positive screening test using visual inspection with acetic acid (VIA). Methods: Opportunistic screening of women (30–50 years) coming to the gynecology clinic by VIA was performed; the screen-positive women were included in the study which had the institutional review board (IRB) approval. A cytology slide for p16Ink4a immunostaining, colposcopy, and biopsy was then performed sequentially. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of p16Ink4a immunocytochemistry were evaluated with histopathology as the gold standard. Results: p16Ink4a positivity showed a linear correlation with the increasing grade of CIN. p16Ink4a positivity was seen in 6% of CIN 1, 80% of CIN 2, 100% of CIN 3, and squamous cell carcinoma. The sensitivity and specificity of p16Ink4a immunocytochemistry for detecting CIN 2 or more was 87.5% (95%CI 61.65–98.45) and 97.06% (95%CI 84.67–99.93). Colposcopy had an equal sensitivity of 87.5% (95% CI 61.65–98.45) and specificity of 50% (95% CI 32.43–67.57), respectively. Conclusion: With high sensitivity and specificity, p16Ink4a immunocytochemistry could be a viable option for triaging VIA-positive women. |
topic |
cervical intraepithelial neoplasia colposcopy human papillomavirus p16ink4a immunocytochemistry visual inspection with acetic acid |
url |
http://www.jcytol.org/article.asp?issn=0970-9371;year=2020;volume=37;issue=2;spage=82;epage=86;aulast=Verma |
work_keys_str_mv |
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