Functional States in Tumor-Initiating Cell Differentiation in Human Colorectal Cancer
Intra-tumor heterogeneity of tumor-initiating cell (TIC) activity drives colorectal cancer (CRC) progression and therapy resistance. Here, we used single-cell RNA-sequencing of patient-derived CRC models to decipher distinct cell subpopulations based on their transcriptional profiles. Cell type-spec...
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Format: | Article |
Language: | English |
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MDPI AG
2021-03-01
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Series: | Cancers |
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Online Access: | https://www.mdpi.com/2072-6694/13/5/1097 |
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doaj-820796e1edaa45718ea43138095a1254 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Martina K. Zowada Stephan M. Tirier Sebastian M. Dieter Teresa G. Krieger Ava Oberlack Robert Lorenz Chua Mario Huerta Foo Wei Ten Karin Laaber Jeongbin Park Katharina Jechow Torsten Müller Mathias Kalxdorf Mark Kriegsmann Katharina Kriegsmann Friederike Herbst Jeroen Krijgsveld Martin Schneider Roland Eils Hanno Glimm Christian Conrad Claudia R. Ball |
spellingShingle |
Martina K. Zowada Stephan M. Tirier Sebastian M. Dieter Teresa G. Krieger Ava Oberlack Robert Lorenz Chua Mario Huerta Foo Wei Ten Karin Laaber Jeongbin Park Katharina Jechow Torsten Müller Mathias Kalxdorf Mark Kriegsmann Katharina Kriegsmann Friederike Herbst Jeroen Krijgsveld Martin Schneider Roland Eils Hanno Glimm Christian Conrad Claudia R. Ball Functional States in Tumor-Initiating Cell Differentiation in Human Colorectal Cancer Cancers colorectal cancer tumor-initiating cells tumor heterogeneity patient-derived cancer models single-cell RNA-sequencing tumor metabolism |
author_facet |
Martina K. Zowada Stephan M. Tirier Sebastian M. Dieter Teresa G. Krieger Ava Oberlack Robert Lorenz Chua Mario Huerta Foo Wei Ten Karin Laaber Jeongbin Park Katharina Jechow Torsten Müller Mathias Kalxdorf Mark Kriegsmann Katharina Kriegsmann Friederike Herbst Jeroen Krijgsveld Martin Schneider Roland Eils Hanno Glimm Christian Conrad Claudia R. Ball |
author_sort |
Martina K. Zowada |
title |
Functional States in Tumor-Initiating Cell Differentiation in Human Colorectal Cancer |
title_short |
Functional States in Tumor-Initiating Cell Differentiation in Human Colorectal Cancer |
title_full |
Functional States in Tumor-Initiating Cell Differentiation in Human Colorectal Cancer |
title_fullStr |
Functional States in Tumor-Initiating Cell Differentiation in Human Colorectal Cancer |
title_full_unstemmed |
Functional States in Tumor-Initiating Cell Differentiation in Human Colorectal Cancer |
title_sort |
functional states in tumor-initiating cell differentiation in human colorectal cancer |
publisher |
MDPI AG |
series |
Cancers |
issn |
2072-6694 |
publishDate |
2021-03-01 |
description |
Intra-tumor heterogeneity of tumor-initiating cell (TIC) activity drives colorectal cancer (CRC) progression and therapy resistance. Here, we used single-cell RNA-sequencing of patient-derived CRC models to decipher distinct cell subpopulations based on their transcriptional profiles. Cell type-specific expression modules of stem-like, transit amplifying-like, and differentiated CRC cells resemble differentiation states of normal intestinal epithelial cells. Strikingly, identified subpopulations differ in proliferative activity and metabolic state. In summary, we here show at single-cell resolution that transcriptional heterogeneity identifies functional states during TIC differentiation. Furthermore, identified expression signatures are linked to patient prognosis. Targeting transcriptional states associated to cancer cell differentiation might unravel novel vulnerabilities in human CRC. |
topic |
colorectal cancer tumor-initiating cells tumor heterogeneity patient-derived cancer models single-cell RNA-sequencing tumor metabolism |
url |
https://www.mdpi.com/2072-6694/13/5/1097 |
work_keys_str_mv |
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doaj-820796e1edaa45718ea43138095a12542021-03-05T00:03:42ZengMDPI AGCancers2072-66942021-03-01131097109710.3390/cancers13051097Functional States in Tumor-Initiating Cell Differentiation in Human Colorectal CancerMartina K. Zowada0Stephan M. Tirier1Sebastian M. Dieter2Teresa G. Krieger3Ava Oberlack4Robert Lorenz Chua5Mario Huerta6Foo Wei Ten7Karin Laaber8Jeongbin Park9Katharina Jechow10Torsten Müller11Mathias Kalxdorf12Mark Kriegsmann13Katharina Kriegsmann14Friederike Herbst15Jeroen Krijgsveld16Martin Schneider17Roland Eils18Hanno Glimm19Christian Conrad20Claudia R. Ball21Translational Functional Cancer Genomics, National Center for Tumor Diseases (NCT) Heidelberg and German Cancer Research Center (DKFZ) Heidelberg, 69120 Heidelberg, GermanyDivision of Theoretical Bioinformatics, DKFZ Heidelberg, 69120 Heidelberg, GermanyTranslational Functional Cancer Genomics, National Center for Tumor Diseases (NCT) Heidelberg and German Cancer Research Center (DKFZ) Heidelberg, 69120 Heidelberg, GermanyDivision of Theoretical Bioinformatics, DKFZ Heidelberg, 69120 Heidelberg, GermanyTranslational Functional Cancer Genomics, National Center for Tumor Diseases (NCT) Heidelberg and German Cancer Research Center (DKFZ) Heidelberg, 69120 Heidelberg, GermanyDivision of Theoretical Bioinformatics, DKFZ Heidelberg, 69120 Heidelberg, GermanyTranslational Functional Cancer Genomics, National Center for Tumor Diseases (NCT) Heidelberg and German Cancer Research Center (DKFZ) Heidelberg, 69120 Heidelberg, GermanyDivision of Theoretical Bioinformatics, DKFZ Heidelberg, 69120 Heidelberg, GermanyTranslational Functional Cancer Genomics, National Center for Tumor Diseases (NCT) Heidelberg and German Cancer Research Center (DKFZ) Heidelberg, 69120 Heidelberg, GermanyDivision of Theoretical Bioinformatics, DKFZ Heidelberg, 69120 Heidelberg, GermanyDivision of Theoretical Bioinformatics, DKFZ Heidelberg, 69120 Heidelberg, GermanyDivision of Proteomics of Stem Cells and Cancer, DKFZ Heidelberg, 69120 Heidelberg, GermanyDivision of Proteomics of Stem Cells and Cancer, DKFZ Heidelberg, 69120 Heidelberg, GermanyInstitute of Pathology, Heidelberg University Hospital, 69120 Heidelberg, GermanyDepartment of Hematology, Oncology and Rheumatology, Heidelberg University Hospital, 69120 Heidelberg, GermanyTranslational Functional Cancer Genomics, National Center for Tumor Diseases (NCT) Heidelberg and German Cancer Research Center (DKFZ) Heidelberg, 69120 Heidelberg, GermanyDivision of Proteomics of Stem Cells and Cancer, DKFZ Heidelberg, 69120 Heidelberg, GermanyDepartment of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, 69120 Heidelberg, GermanyDivision of Theoretical Bioinformatics, DKFZ Heidelberg, 69120 Heidelberg, GermanyTranslational Functional Cancer Genomics, National Center for Tumor Diseases (NCT) Heidelberg and German Cancer Research Center (DKFZ) Heidelberg, 69120 Heidelberg, GermanyDivision of Theoretical Bioinformatics, DKFZ Heidelberg, 69120 Heidelberg, GermanyTranslational Functional Cancer Genomics, National Center for Tumor Diseases (NCT) Heidelberg and German Cancer Research Center (DKFZ) Heidelberg, 69120 Heidelberg, GermanyIntra-tumor heterogeneity of tumor-initiating cell (TIC) activity drives colorectal cancer (CRC) progression and therapy resistance. Here, we used single-cell RNA-sequencing of patient-derived CRC models to decipher distinct cell subpopulations based on their transcriptional profiles. Cell type-specific expression modules of stem-like, transit amplifying-like, and differentiated CRC cells resemble differentiation states of normal intestinal epithelial cells. Strikingly, identified subpopulations differ in proliferative activity and metabolic state. In summary, we here show at single-cell resolution that transcriptional heterogeneity identifies functional states during TIC differentiation. Furthermore, identified expression signatures are linked to patient prognosis. Targeting transcriptional states associated to cancer cell differentiation might unravel novel vulnerabilities in human CRC.https://www.mdpi.com/2072-6694/13/5/1097colorectal cancertumor-initiating cellstumor heterogeneitypatient-derived cancer modelssingle-cell RNA-sequencingtumor metabolism |