Intervertebral Disc Regeneration Injection of a Cell-Loaded Collagen Hydrogel in a Sheep Model
Degenerated intervertebral discs (IVDs) were treated with autologous adipose-derived stem cells (ASC) loaded into an injectable collagen scaffold in a sheep model to investigate the implant’s therapeutic potential regarding the progression of degeneration of previously damaged discs. In this study,...
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doaj-822b092d003c440e803369ef6207dc572021-04-19T23:06:02ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01224248424810.3390/ijms22084248Intervertebral Disc Regeneration Injection of a Cell-Loaded Collagen Hydrogel in a Sheep ModelAndrea Friedmann0Andre Baertel1Christine Schmitt2Christopher Ludtka3Javorina Milosevic4Hans-Joerg Meisel5Felix Goehre6Stefan Schwan7Department of Biological and Macromolecular Materials, Fraunhofer Institute for Microstructure of Materials and Systems IMWS, 06120 Halle, GermanyDepartment of Veterinary Medicine, University of Leipzig, 04103 Leipzig, GermanyDepartment of Biological and Macromolecular Materials, Fraunhofer Institute for Microstructure of Materials and Systems IMWS, 06120 Halle, GermanyDepartment of Biomedical Engineering, University of Florida, Gainesville, FL 32611, USASpinplant GmbH, 06120 Halle, GermanyDepartment of Neurosurgery, BG Klinikum Bergmannstrost, 06110 Halle, GermanyDepartment of Neurosurgery, BG Klinikum Bergmannstrost, 06110 Halle, GermanyDepartment of Biological and Macromolecular Materials, Fraunhofer Institute for Microstructure of Materials and Systems IMWS, 06120 Halle, GermanyDegenerated intervertebral discs (IVDs) were treated with autologous adipose-derived stem cells (ASC) loaded into an injectable collagen scaffold in a sheep model to investigate the implant’s therapeutic potential regarding the progression of degeneration of previously damaged discs. In this study, 18 merino sheep were subjected to a 3-step minimally invasive injury and treatment model, which consisted of surgically induced disc degeneration, treatment of IVDs with an ASC-loaded collagen hydrogel 6 weeks post-operatively, and assessment of the implant’s influence on degenerative tissue changes after 6 and 12 months of grazing. Autologous ASCs were extracted from subcutaneous adipose tissue and cultivated in vitro. At the end of the experiment, disc heights were determined by µ-CT measurements and morphological tissue changes were histologically examined.Histological investigations show that, after treatment with the ASC-loaded collagen hydrogel implant, degeneration-specific features were observed less frequently. Quantitative studies of the degree of degeneration did not demonstrate a significant influence on potential tissue regeneration with treatment. Regarding disc height analysis, at both 6 and 12 months after treatment with the ASC-loaded collagen hydrogel implant a stabilization of the disc height can be seen. A complete restoration of the intervertebral disc heights however could not be achieved.The reported injection procedure describes in a preclinical model a translational therapeutic approach for degenerative disc diseases based on adipose-derived stem cells in a collagen hydrogel scaffold. Further investigations are planned with the use of a different injectable scaffold material using the same test model.https://www.mdpi.com/1422-0067/22/8/4248adipose stem cellcollagen hydrogelintervertebral disc regenerationinjectionovine modelregenerative medicine |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Andrea Friedmann Andre Baertel Christine Schmitt Christopher Ludtka Javorina Milosevic Hans-Joerg Meisel Felix Goehre Stefan Schwan |
spellingShingle |
Andrea Friedmann Andre Baertel Christine Schmitt Christopher Ludtka Javorina Milosevic Hans-Joerg Meisel Felix Goehre Stefan Schwan Intervertebral Disc Regeneration Injection of a Cell-Loaded Collagen Hydrogel in a Sheep Model International Journal of Molecular Sciences adipose stem cell collagen hydrogel intervertebral disc regeneration injection ovine model regenerative medicine |
author_facet |
Andrea Friedmann Andre Baertel Christine Schmitt Christopher Ludtka Javorina Milosevic Hans-Joerg Meisel Felix Goehre Stefan Schwan |
author_sort |
Andrea Friedmann |
title |
Intervertebral Disc Regeneration Injection of a Cell-Loaded Collagen Hydrogel in a Sheep Model |
title_short |
Intervertebral Disc Regeneration Injection of a Cell-Loaded Collagen Hydrogel in a Sheep Model |
title_full |
Intervertebral Disc Regeneration Injection of a Cell-Loaded Collagen Hydrogel in a Sheep Model |
title_fullStr |
Intervertebral Disc Regeneration Injection of a Cell-Loaded Collagen Hydrogel in a Sheep Model |
title_full_unstemmed |
Intervertebral Disc Regeneration Injection of a Cell-Loaded Collagen Hydrogel in a Sheep Model |
title_sort |
intervertebral disc regeneration injection of a cell-loaded collagen hydrogel in a sheep model |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-04-01 |
description |
Degenerated intervertebral discs (IVDs) were treated with autologous adipose-derived stem cells (ASC) loaded into an injectable collagen scaffold in a sheep model to investigate the implant’s therapeutic potential regarding the progression of degeneration of previously damaged discs. In this study, 18 merino sheep were subjected to a 3-step minimally invasive injury and treatment model, which consisted of surgically induced disc degeneration, treatment of IVDs with an ASC-loaded collagen hydrogel 6 weeks post-operatively, and assessment of the implant’s influence on degenerative tissue changes after 6 and 12 months of grazing. Autologous ASCs were extracted from subcutaneous adipose tissue and cultivated in vitro. At the end of the experiment, disc heights were determined by µ-CT measurements and morphological tissue changes were histologically examined.Histological investigations show that, after treatment with the ASC-loaded collagen hydrogel implant, degeneration-specific features were observed less frequently. Quantitative studies of the degree of degeneration did not demonstrate a significant influence on potential tissue regeneration with treatment. Regarding disc height analysis, at both 6 and 12 months after treatment with the ASC-loaded collagen hydrogel implant a stabilization of the disc height can be seen. A complete restoration of the intervertebral disc heights however could not be achieved.The reported injection procedure describes in a preclinical model a translational therapeutic approach for degenerative disc diseases based on adipose-derived stem cells in a collagen hydrogel scaffold. Further investigations are planned with the use of a different injectable scaffold material using the same test model. |
topic |
adipose stem cell collagen hydrogel intervertebral disc regeneration injection ovine model regenerative medicine |
url |
https://www.mdpi.com/1422-0067/22/8/4248 |
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