Rhamnolipid coating reduces microbial biofilm formation on titanium implants: an in vitro study
Abstract Background Peri-implant mucositis and peri-implantitis are biofilm-related diseases causing major concern in oral implantology, requiring complex anti-infective procedures or implant removal. Microbial biosurfactants emerged as new anti-biofilm agents for coating implantable devices preserv...
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doaj-8245743483194d098fc91e77c500b0082021-02-07T12:44:35ZengBMCBMC Oral Health1472-68312021-02-0121111310.1186/s12903-021-01412-7Rhamnolipid coating reduces microbial biofilm formation on titanium implants: an in vitro studyErica Tambone0Emiliana Bonomi1Paolo Ghensi2Devid Maniglio3Chiara Ceresa4Francesca Agostinacchio5Patrizio Caciagli6Giandomenico Nollo7Federico Piccoli8Iole Caola9Letizia Fracchia10Francesco Tessarolo11Department of Industrial Engineering and BIOtech, University of TrentoDepartment of Industrial Engineering and BIOtech, University of TrentoDepartment CIBIO, University of TrentoDepartment of Industrial Engineering and BIOtech, University of TrentoDepartment of Pharmaceutical Sciences, Università del Piemonte Orientale “A. Avogadro”Department of Industrial Engineering and BIOtech, University of TrentoDepartment of Laboratory Medicine, Azienda Provinciale Per I Servizi SanitariDepartment of Industrial Engineering and BIOtech, University of TrentoDepartment of Laboratory Medicine, Azienda Provinciale Per I Servizi SanitariDepartment of Laboratory Medicine, Azienda Provinciale Per I Servizi SanitariDepartment of Pharmaceutical Sciences, Università del Piemonte Orientale “A. Avogadro”Department of Industrial Engineering and BIOtech, University of TrentoAbstract Background Peri-implant mucositis and peri-implantitis are biofilm-related diseases causing major concern in oral implantology, requiring complex anti-infective procedures or implant removal. Microbial biosurfactants emerged as new anti-biofilm agents for coating implantable devices preserving biocompatibility. This study aimed to assess the efficacy of rhamnolipid biosurfactant R89 (R89BS) to reduce Staphylococcus aureus and Staphylococcus epidermidis biofilm formation on titanium. Methods R89BS was physically adsorbed on titanium discs (TDs). Cytotoxicity of coated TDs was evaluated on normal lung fibroblasts (MRC5) using a lactate dehydrogenase assay. The ability of coated TDs to inhibit biofilm formation was evaluated by quantifying biofilm biomass and cell metabolic activity, at different time-points, with respect to uncoated controls. A qualitative analysis of sessile bacteria was also performed by scanning electron microscopy. Results R89BS-coated discs showed no cytotoxic effects. TDs coated with 4 mg/mL R89BS inhibited the biofilm biomass of S. aureus by 99%, 47% and 7% and of S. epidermidis by 54%, 29%, and 10% at 24, 48 and 72 h respectively. A significant reduction of the biofilm metabolic activity was also documented. The same coating applied on three commercial implant surfaces resulted in a biomass inhibition higher than 90% for S. aureus, and up to 78% for S. epidermidis at 24 h. Conclusions R89BS-coating was effective in reducing Staphylococcus biofilm formation at the titanium implant surface. The anti-biofilm action can be obtained on several different commercially available implant surfaces, independently of their surface morphology.https://doi.org/10.1186/s12903-021-01412-7Dental implantsBiofilmTitaniumBiosurfactantStaphylococcus spp |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Erica Tambone Emiliana Bonomi Paolo Ghensi Devid Maniglio Chiara Ceresa Francesca Agostinacchio Patrizio Caciagli Giandomenico Nollo Federico Piccoli Iole Caola Letizia Fracchia Francesco Tessarolo |
spellingShingle |
Erica Tambone Emiliana Bonomi Paolo Ghensi Devid Maniglio Chiara Ceresa Francesca Agostinacchio Patrizio Caciagli Giandomenico Nollo Federico Piccoli Iole Caola Letizia Fracchia Francesco Tessarolo Rhamnolipid coating reduces microbial biofilm formation on titanium implants: an in vitro study BMC Oral Health Dental implants Biofilm Titanium Biosurfactant Staphylococcus spp |
author_facet |
Erica Tambone Emiliana Bonomi Paolo Ghensi Devid Maniglio Chiara Ceresa Francesca Agostinacchio Patrizio Caciagli Giandomenico Nollo Federico Piccoli Iole Caola Letizia Fracchia Francesco Tessarolo |
author_sort |
Erica Tambone |
title |
Rhamnolipid coating reduces microbial biofilm formation on titanium implants: an in vitro study |
title_short |
Rhamnolipid coating reduces microbial biofilm formation on titanium implants: an in vitro study |
title_full |
Rhamnolipid coating reduces microbial biofilm formation on titanium implants: an in vitro study |
title_fullStr |
Rhamnolipid coating reduces microbial biofilm formation on titanium implants: an in vitro study |
title_full_unstemmed |
Rhamnolipid coating reduces microbial biofilm formation on titanium implants: an in vitro study |
title_sort |
rhamnolipid coating reduces microbial biofilm formation on titanium implants: an in vitro study |
publisher |
BMC |
series |
BMC Oral Health |
issn |
1472-6831 |
publishDate |
2021-02-01 |
description |
Abstract Background Peri-implant mucositis and peri-implantitis are biofilm-related diseases causing major concern in oral implantology, requiring complex anti-infective procedures or implant removal. Microbial biosurfactants emerged as new anti-biofilm agents for coating implantable devices preserving biocompatibility. This study aimed to assess the efficacy of rhamnolipid biosurfactant R89 (R89BS) to reduce Staphylococcus aureus and Staphylococcus epidermidis biofilm formation on titanium. Methods R89BS was physically adsorbed on titanium discs (TDs). Cytotoxicity of coated TDs was evaluated on normal lung fibroblasts (MRC5) using a lactate dehydrogenase assay. The ability of coated TDs to inhibit biofilm formation was evaluated by quantifying biofilm biomass and cell metabolic activity, at different time-points, with respect to uncoated controls. A qualitative analysis of sessile bacteria was also performed by scanning electron microscopy. Results R89BS-coated discs showed no cytotoxic effects. TDs coated with 4 mg/mL R89BS inhibited the biofilm biomass of S. aureus by 99%, 47% and 7% and of S. epidermidis by 54%, 29%, and 10% at 24, 48 and 72 h respectively. A significant reduction of the biofilm metabolic activity was also documented. The same coating applied on three commercial implant surfaces resulted in a biomass inhibition higher than 90% for S. aureus, and up to 78% for S. epidermidis at 24 h. Conclusions R89BS-coating was effective in reducing Staphylococcus biofilm formation at the titanium implant surface. The anti-biofilm action can be obtained on several different commercially available implant surfaces, independently of their surface morphology. |
topic |
Dental implants Biofilm Titanium Biosurfactant Staphylococcus spp |
url |
https://doi.org/10.1186/s12903-021-01412-7 |
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