Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse
<p>Abstract</p> <p>Background</p> <p>The equine periodontium provides tooth support and lifelong tooth eruption on a remarkable scale. These functions require continuous tissue remodeling. It is assumed that multipotent mesenchymal stromal cells (MSC) reside in the peri...
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doaj-824c5c2afe3242f29b9108f7835b48c52020-11-24T20:47:26ZengBMCBMC Veterinary Research1746-61482011-08-01714210.1186/1746-6148-7-42Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horsePfarrer ChristianeHaeger Jan-DirkHambruch NinaGasse HagenMensing NielsStaszyk Carsten<p>Abstract</p> <p>Background</p> <p>The equine periodontium provides tooth support and lifelong tooth eruption on a remarkable scale. These functions require continuous tissue remodeling. It is assumed that multipotent mesenchymal stromal cells (MSC) reside in the periodontal ligament (PDL) and play a crucial role in regulating physiological periodontal tissue regeneration. The aim of this study was to isolate and characterize equine periodontal MSC.</p> <p>Tissue samples were obtained from four healthy horses. Primary cell populations were har-vested and cultured from the gingiva, from three horizontal levels of the PDL (apical, midtooth and subgingival) and for comparison purposes from the subcutis (masseteric region). Colony-forming cells were grown on uncoated culture dishes and typical <it>in vitro </it>characteristics of non-human MSC, i.e. self-renewal capacity, population doubling time, expression of stemness markers and trilineage differentiation were analyzed.</p> <p>Results</p> <p>Colony-forming cell populations from all locations showed expression of the stemness markers CD90 and CD105. In vitro self-renewal capacity was demonstrated by colony-forming unit fibroblast (CFU-F) assays. CFU-efficiency was highest in cell populations from the apical and from the mid-tooth PDL. Population doubling time was highest in subcutaneous cells. All investigated cell populations possessed trilineage differentiation potential into osteogenic, adipogenic and chondrogenic lineages.</p> <p>Conclusions</p> <p>Due to the demonstrated in vitro characteristics cells were referred to as equine subcutaneous MSC (eSc-MSC), equine gingival MSC (eG-MSC) and equine periodontal MSC (eP-MSC). According to different PDL levels, eP-MSC were further specified as eP-MSC from the apical PDL (eP-MSCap), eP-MSC from the mid-tooth PDL (eP-MSCm) and eP-MSC from the subgingival PDL (eP-MSCsg). Considering current concepts of cell-based regenerative therapies in horses, eP-MSC might be promising candidates for future clinical applications in equine orthopedic and periodontal diseases.</p> http://www.biomedcentral.com/1746-6148/7/42 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pfarrer Christiane Haeger Jan-Dirk Hambruch Nina Gasse Hagen Mensing Niels Staszyk Carsten |
spellingShingle |
Pfarrer Christiane Haeger Jan-Dirk Hambruch Nina Gasse Hagen Mensing Niels Staszyk Carsten Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse BMC Veterinary Research |
author_facet |
Pfarrer Christiane Haeger Jan-Dirk Hambruch Nina Gasse Hagen Mensing Niels Staszyk Carsten |
author_sort |
Pfarrer Christiane |
title |
Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse |
title_short |
Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse |
title_full |
Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse |
title_fullStr |
Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse |
title_full_unstemmed |
Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse |
title_sort |
isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse |
publisher |
BMC |
series |
BMC Veterinary Research |
issn |
1746-6148 |
publishDate |
2011-08-01 |
description |
<p>Abstract</p> <p>Background</p> <p>The equine periodontium provides tooth support and lifelong tooth eruption on a remarkable scale. These functions require continuous tissue remodeling. It is assumed that multipotent mesenchymal stromal cells (MSC) reside in the periodontal ligament (PDL) and play a crucial role in regulating physiological periodontal tissue regeneration. The aim of this study was to isolate and characterize equine periodontal MSC.</p> <p>Tissue samples were obtained from four healthy horses. Primary cell populations were har-vested and cultured from the gingiva, from three horizontal levels of the PDL (apical, midtooth and subgingival) and for comparison purposes from the subcutis (masseteric region). Colony-forming cells were grown on uncoated culture dishes and typical <it>in vitro </it>characteristics of non-human MSC, i.e. self-renewal capacity, population doubling time, expression of stemness markers and trilineage differentiation were analyzed.</p> <p>Results</p> <p>Colony-forming cell populations from all locations showed expression of the stemness markers CD90 and CD105. In vitro self-renewal capacity was demonstrated by colony-forming unit fibroblast (CFU-F) assays. CFU-efficiency was highest in cell populations from the apical and from the mid-tooth PDL. Population doubling time was highest in subcutaneous cells. All investigated cell populations possessed trilineage differentiation potential into osteogenic, adipogenic and chondrogenic lineages.</p> <p>Conclusions</p> <p>Due to the demonstrated in vitro characteristics cells were referred to as equine subcutaneous MSC (eSc-MSC), equine gingival MSC (eG-MSC) and equine periodontal MSC (eP-MSC). According to different PDL levels, eP-MSC were further specified as eP-MSC from the apical PDL (eP-MSCap), eP-MSC from the mid-tooth PDL (eP-MSCm) and eP-MSC from the subgingival PDL (eP-MSCsg). Considering current concepts of cell-based regenerative therapies in horses, eP-MSC might be promising candidates for future clinical applications in equine orthopedic and periodontal diseases.</p> |
url |
http://www.biomedcentral.com/1746-6148/7/42 |
work_keys_str_mv |
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