GmSYP24, a putative syntaxin gene, confers osmotic/drought, salt stress tolerances and ABA signal pathway
Abstract As major environment factors, drought or high salinity affect crop growth, development and yield. Transgenic approach is an effective way to improve abiotic stress tolerance of crops. In this study, we comparatively analyzed gene structures, genome location, and the evolution of syntaxin pr...
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doaj-825cf277fe8d44928efd8d4609ffc0712020-12-08T06:43:31ZengNature Publishing GroupScientific Reports2045-23222019-04-019111210.1038/s41598-019-42332-5GmSYP24, a putative syntaxin gene, confers osmotic/drought, salt stress tolerances and ABA signal pathwayLi-Miao Chen0Yi-Sheng Fang1Chan-Juan Zhang2Qing-Nan Hao3Dong Cao4Song-Li Yuan5Hai-Feng Chen6Zhong-Lu Yang7Shui-Lian Chen8Zhi-Hui Shan9Bao-Hong Liu10Jing-Wang11Yong Zhan12Xiao-Juan Zhang13De-Zhen Qiu14Wen-Bin Li15Xin-An Zhou16Key Laboratory of Oil Crop Biology, Ministry of AgricultureKey Laboratory of Oil Crop Biology, Ministry of AgricultureKey Laboratory of Oil Crop Biology, Ministry of AgricultureKey Laboratory of Oil Crop Biology, Ministry of AgricultureKey Laboratory of Oil Crop Biology, Ministry of AgricultureKey Laboratory of Oil Crop Biology, Ministry of AgricultureKey Laboratory of Oil Crop Biology, Ministry of AgricultureKey Laboratory of Oil Crop Biology, Ministry of AgricultureKey Laboratory of Oil Crop Biology, Ministry of AgricultureKey Laboratory of Oil Crop Biology, Ministry of AgricultureKey Laboratory of Oil Crop Biology, Ministry of AgricultureKey Laboratory of Oil Crop Biology, Ministry of AgricultureCrop Research Institute, Xinjiang Academy of Agricultural and Reclamation Science, Key Lab of Cereal Quality Research and Genetic Improvement, Xinjiang Production and Construction CropsKey Laboratory of Oil Crop Biology, Ministry of AgricultureKey Laboratory of Oil Crop Biology, Ministry of AgricultureKey Laboratory of Soybean Biology in the Chinese Ministry of Education, Northeast Agricultural UniversityKey Laboratory of Oil Crop Biology, Ministry of AgricultureAbstract As major environment factors, drought or high salinity affect crop growth, development and yield. Transgenic approach is an effective way to improve abiotic stress tolerance of crops. In this study, we comparatively analyzed gene structures, genome location, and the evolution of syntaxin proteins containing late embryogenesis abundant (LEA2) domain. GmSYP24 was identified as a dehydration-responsive gene. Our study showed that the GmSYP24 protein was located on the cell membrane. The overexpression of GmSYP24 (GmSYP24ox) in soybean and heteroexpression of GmSYP24 (GmSYP24hx) in Arabidopsis exhibited insensitivity to osmotic/drought and high salinity. However, wild type soybean, Arabidopsis, and the mutant of GmSYP24 homologous gene of Arabidopsis were sensitive to the stresses. Under the abiotic stresses, transgenic soybean plants had greater water content and higher activities of POD, SOD compared with non-transgenic controls. And the leaf stomatal density and opening were reduced in transgenic Arabidopsis. The sensitivity to ABA was decreased during seed germination of GmSYP24ox and GmSYP24hx. GmSYP24hx induced up-regulation of ABA-responsive genes. GmSYP24ox alters the expression of some aquaporins under osmotic/drought, salt, or ABA treatment. These results demonstrated that GmSYP24 played an important role in osmotic/drought or salt tolerance in ABA signal pathway.https://doi.org/10.1038/s41598-019-42332-5 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Li-Miao Chen Yi-Sheng Fang Chan-Juan Zhang Qing-Nan Hao Dong Cao Song-Li Yuan Hai-Feng Chen Zhong-Lu Yang Shui-Lian Chen Zhi-Hui Shan Bao-Hong Liu Jing-Wang Yong Zhan Xiao-Juan Zhang De-Zhen Qiu Wen-Bin Li Xin-An Zhou |
spellingShingle |
Li-Miao Chen Yi-Sheng Fang Chan-Juan Zhang Qing-Nan Hao Dong Cao Song-Li Yuan Hai-Feng Chen Zhong-Lu Yang Shui-Lian Chen Zhi-Hui Shan Bao-Hong Liu Jing-Wang Yong Zhan Xiao-Juan Zhang De-Zhen Qiu Wen-Bin Li Xin-An Zhou GmSYP24, a putative syntaxin gene, confers osmotic/drought, salt stress tolerances and ABA signal pathway Scientific Reports |
author_facet |
Li-Miao Chen Yi-Sheng Fang Chan-Juan Zhang Qing-Nan Hao Dong Cao Song-Li Yuan Hai-Feng Chen Zhong-Lu Yang Shui-Lian Chen Zhi-Hui Shan Bao-Hong Liu Jing-Wang Yong Zhan Xiao-Juan Zhang De-Zhen Qiu Wen-Bin Li Xin-An Zhou |
author_sort |
Li-Miao Chen |
title |
GmSYP24, a putative syntaxin gene, confers osmotic/drought, salt stress tolerances and ABA signal pathway |
title_short |
GmSYP24, a putative syntaxin gene, confers osmotic/drought, salt stress tolerances and ABA signal pathway |
title_full |
GmSYP24, a putative syntaxin gene, confers osmotic/drought, salt stress tolerances and ABA signal pathway |
title_fullStr |
GmSYP24, a putative syntaxin gene, confers osmotic/drought, salt stress tolerances and ABA signal pathway |
title_full_unstemmed |
GmSYP24, a putative syntaxin gene, confers osmotic/drought, salt stress tolerances and ABA signal pathway |
title_sort |
gmsyp24, a putative syntaxin gene, confers osmotic/drought, salt stress tolerances and aba signal pathway |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2019-04-01 |
description |
Abstract As major environment factors, drought or high salinity affect crop growth, development and yield. Transgenic approach is an effective way to improve abiotic stress tolerance of crops. In this study, we comparatively analyzed gene structures, genome location, and the evolution of syntaxin proteins containing late embryogenesis abundant (LEA2) domain. GmSYP24 was identified as a dehydration-responsive gene. Our study showed that the GmSYP24 protein was located on the cell membrane. The overexpression of GmSYP24 (GmSYP24ox) in soybean and heteroexpression of GmSYP24 (GmSYP24hx) in Arabidopsis exhibited insensitivity to osmotic/drought and high salinity. However, wild type soybean, Arabidopsis, and the mutant of GmSYP24 homologous gene of Arabidopsis were sensitive to the stresses. Under the abiotic stresses, transgenic soybean plants had greater water content and higher activities of POD, SOD compared with non-transgenic controls. And the leaf stomatal density and opening were reduced in transgenic Arabidopsis. The sensitivity to ABA was decreased during seed germination of GmSYP24ox and GmSYP24hx. GmSYP24hx induced up-regulation of ABA-responsive genes. GmSYP24ox alters the expression of some aquaporins under osmotic/drought, salt, or ABA treatment. These results demonstrated that GmSYP24 played an important role in osmotic/drought or salt tolerance in ABA signal pathway. |
url |
https://doi.org/10.1038/s41598-019-42332-5 |
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