Nutritional Ingredients Modulate Adipokine Secretion and Inflammation in Human Primary Adipocytes
Nutritional factors such as casein hydrolysates and long chain polyunsaturated fatty acids have been proposed to exert beneficial metabolic effects. We aimed to investigate how a casein hydrolysate (eCH) and long chain polyunsaturated fatty acids could affect human primary adipocyte function in vitr...
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doaj-827fe743c5be4c12b8793ae2d4c267562020-11-25T00:46:41ZengMDPI AGNutrients2072-66432015-01-017286588610.3390/nu7020865nu7020865Nutritional Ingredients Modulate Adipokine Secretion and Inflammation in Human Primary AdipocytesTania Romacho0Philipp Glosse1Isabel Richter2Manuela Elsen3Marieke H. Schoemaker4Eric A. van Tol5Jürgen Eckel6Paul Langerhans Group for Integrative Physiology, German Diabetes Center, Auf'm Hennekamp 65, 40225 Düsseldorf, GermanyPaul Langerhans Group for Integrative Physiology, German Diabetes Center, Auf'm Hennekamp 65, 40225 Düsseldorf, GermanyPaul Langerhans Group for Integrative Physiology, German Diabetes Center, Auf'm Hennekamp 65, 40225 Düsseldorf, GermanyPaul Langerhans Group for Integrative Physiology, German Diabetes Center, Auf'm Hennekamp 65, 40225 Düsseldorf, GermanyGlobal Discovery, Mead Johnson Pediatric Nutrition Institute, Middenkampweg 2, Nijmegen, 6545 CJ, The NetherlandsGlobal Discovery, Mead Johnson Pediatric Nutrition Institute, Middenkampweg 2, Nijmegen, 6545 CJ, The NetherlandsPaul Langerhans Group for Integrative Physiology, German Diabetes Center, Auf'm Hennekamp 65, 40225 Düsseldorf, GermanyNutritional factors such as casein hydrolysates and long chain polyunsaturated fatty acids have been proposed to exert beneficial metabolic effects. We aimed to investigate how a casein hydrolysate (eCH) and long chain polyunsaturated fatty acids could affect human primary adipocyte function in vitro. Incubation conditions with the different nutritional factors were validated by assessing cell vitality with lactate dehydrogenase (LDH) release and neutral red incorporation. Intracellular triglyceride content was assessed with Oil Red O staining. The effect of eCH, a non-peptidic amino acid mixture (AA), and long-chain polyunsaturated fatty acids (LC-PUFAs) on adiponectin and leptin secretion was determined by enzyme-linked immunosorbent assay (ELISA). Intracellular adiponectin expression and nuclear factor-κB (NF-κB) activation were analyzed by Western blot, while monocyte chemoattractant protein-1 (MCP-1) release was explored by ELISA. The eCH concentration dependently increased adiponectin secretion in human primary adipocytes through its intrinsic peptide bioactivity, since the non-peptidic mixture, AA, could not mimic eCH’s effects on adiponectin secretion. Eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and DHA combined with arachidonic acid (ARA) upregulated adiponectin secretion. However, only DHA and DHA/ARA exerted a potentanti-inflammatory effect reflected by prevention of tumor necrosis factor-α (TNF-α) induced NF-κB activation and MCP-1 secretion in human adipocytes. eCH and DHA alone or in combination with ARA, may hold the key for nutritional programming through their anti-inflammatory action to prevent diseases with low-grade chronic inflammation such as obesity or diabetes.http://www.mdpi.com/2072-6643/7/2/865milk proteinscasein hydrolysateEPADHAARALC-PUFAsadiponectinadipocytes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tania Romacho Philipp Glosse Isabel Richter Manuela Elsen Marieke H. Schoemaker Eric A. van Tol Jürgen Eckel |
spellingShingle |
Tania Romacho Philipp Glosse Isabel Richter Manuela Elsen Marieke H. Schoemaker Eric A. van Tol Jürgen Eckel Nutritional Ingredients Modulate Adipokine Secretion and Inflammation in Human Primary Adipocytes Nutrients milk proteins casein hydrolysate EPA DHA ARA LC-PUFAs adiponectin adipocytes |
author_facet |
Tania Romacho Philipp Glosse Isabel Richter Manuela Elsen Marieke H. Schoemaker Eric A. van Tol Jürgen Eckel |
author_sort |
Tania Romacho |
title |
Nutritional Ingredients Modulate Adipokine Secretion and Inflammation in Human Primary Adipocytes |
title_short |
Nutritional Ingredients Modulate Adipokine Secretion and Inflammation in Human Primary Adipocytes |
title_full |
Nutritional Ingredients Modulate Adipokine Secretion and Inflammation in Human Primary Adipocytes |
title_fullStr |
Nutritional Ingredients Modulate Adipokine Secretion and Inflammation in Human Primary Adipocytes |
title_full_unstemmed |
Nutritional Ingredients Modulate Adipokine Secretion and Inflammation in Human Primary Adipocytes |
title_sort |
nutritional ingredients modulate adipokine secretion and inflammation in human primary adipocytes |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2015-01-01 |
description |
Nutritional factors such as casein hydrolysates and long chain polyunsaturated fatty acids have been proposed to exert beneficial metabolic effects. We aimed to investigate how a casein hydrolysate (eCH) and long chain polyunsaturated fatty acids could affect human primary adipocyte function in vitro. Incubation conditions with the different nutritional factors were validated by assessing cell vitality with lactate dehydrogenase (LDH) release and neutral red incorporation. Intracellular triglyceride content was assessed with Oil Red O staining. The effect of eCH, a non-peptidic amino acid mixture (AA), and long-chain polyunsaturated fatty acids (LC-PUFAs) on adiponectin and leptin secretion was determined by enzyme-linked immunosorbent assay (ELISA). Intracellular adiponectin expression and nuclear factor-κB (NF-κB) activation were analyzed by Western blot, while monocyte chemoattractant protein-1 (MCP-1) release was explored by ELISA. The eCH concentration dependently increased adiponectin secretion in human primary adipocytes through its intrinsic peptide bioactivity, since the non-peptidic mixture, AA, could not mimic eCH’s effects on adiponectin secretion. Eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and DHA combined with arachidonic acid (ARA) upregulated adiponectin secretion. However, only DHA and DHA/ARA exerted a potentanti-inflammatory effect reflected by prevention of tumor necrosis factor-α (TNF-α) induced NF-κB activation and MCP-1 secretion in human adipocytes. eCH and DHA alone or in combination with ARA, may hold the key for nutritional programming through their anti-inflammatory action to prevent diseases with low-grade chronic inflammation such as obesity or diabetes. |
topic |
milk proteins casein hydrolysate EPA DHA ARA LC-PUFAs adiponectin adipocytes |
url |
http://www.mdpi.com/2072-6643/7/2/865 |
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