INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia lentiscus L.) FROM TURKEY
In this study, intra-specific variations in naturally growing and cultivated mastic tree (Pistacia lentiscus L.) samples obtained from western parts of Turkey were examined using ISSR and IRAP marker techniques. Samples from Crete and Chios were also included in the study. Morphological measurements...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Trakya University
2018-10-01
|
| Series: | Trakya University Journal of Natural Sciences |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/en/pub/trkjnat/issue/39729/433329 |
| Summary: | In this study, intra-specific variations in
naturally growing and cultivated mastic tree (Pistacia lentiscus L.) samples obtained from western parts of
Turkey were examined using ISSR and IRAP marker techniques. Samples from Crete
and Chios were also included in the study. Morphological measurements of some
leaf characteristics of the samples were performed and the measured data was evaluated
statistically with a Pearson Correlation analysis to reveal the correlations
between character pairs. ISSR primers produced 81 bands between 161-1884bp with
96.3% polymorphism and IRAP primers produced 72 bands between
124-2027bp with 91.67% polymorphism. Polymorphism information content (PIC)
values were 0.458 and 0.418 for ISSR and IRAP, respectively. Genetic similarity
matrix was examined with Jaccard’s coefficient. Maximum similarity was found
between the Cretan samples (LG2 and LG3) with the ISSR analysis (0.933) and
between L25A (C1, Bodrum) and L29A (C1, Milas) with the IRAP analysis (0.593).
Unweighted pair group method with arithmetic mean (UPGMA) dendrogram was
divided into 12 and 4 groups by ISSR and IRAP methods, respectively. Specimens
were segregated on 3 main different clusters by the Principal Component
Analysis (PCA) based on the combined marker systems. The results showed that P. lentiscus has very high ratios of
intraspecific variation. The present work is an original study in terms of
large sampling including wild genotypes, cultivated specimen, Chios and Cretan
varieties, use of ISSR and IRAP combination, determination of relations between
culture and wild genotypes and the use of Bagy-1
retrotransposons in intraspecific polymorphism. This study may be considered as
a reference study for studies on gene pools of P. lentiscus and phylogenetic relationships within the species and
may contribute to species concept and agricultural breeding programs. |
|---|---|
| ISSN: | 2147-0294 2528-9691 |