Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms.

A simple modification of the Dittmer-Lester reagent is described that allow the detection of phospholipid derivatives at very low concentrations on silica gel and reversed-phase thin-layer plates. This modification, which involves the addition of acetic acid to the mixture, permits the observation o...

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Main Authors:	E K Ryu, M MacCoss
Format:	Article
Language:	English
Published:	Elsevier 1979-05-01
Series:	Journal of Lipid Research
Online Access:	http://www.sciencedirect.com/science/article/pii/S0022227520406121

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record_format	Article
spelling	doaj-82992b379d8b490c8b62aa46c582204a2021-04-24T05:52:53ZengElsevierJournal of Lipid Research0022-22751979-05-01204561563Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms.E K RyuM MacCossA simple modification of the Dittmer-Lester reagent is described that allow the detection of phospholipid derivatives at very low concentrations on silica gel and reversed-phase thin-layer plates. This modification, which involves the addition of acetic acid to the mixture, permits the observation of sharp blue spots on a white background. The specificity and sensitivity of the spray are discussed.http://www.sciencedirect.com/science/article/pii/S0022227520406121
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	E K Ryu M MacCoss
spellingShingle	E K Ryu M MacCoss Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms. Journal of Lipid Research
author_facet	E K Ryu M MacCoss
author_sort	E K Ryu
title	Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms.
title_short	Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms.
title_full	Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms.
title_fullStr	Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms.
title_full_unstemmed	Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms.
title_sort	modification of the dittmer-lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms.
publisher	Elsevier
series	Journal of Lipid Research
issn	0022-2275
publishDate	1979-05-01
description	A simple modification of the Dittmer-Lester reagent is described that allow the detection of phospholipid derivatives at very low concentrations on silica gel and reversed-phase thin-layer plates. This modification, which involves the addition of acetic acid to the mixture, permits the observation of sharp blue spots on a white background. The specificity and sensitivity of the spray are discussed.
url	http://www.sciencedirect.com/science/article/pii/S0022227520406121
work_keys_str_mv	AT ekryu modificationofthedittmerlesterreagentforthedetectionofphospholipidderivativesonthinlayerchromatograms AT mmaccoss modificationofthedittmerlesterreagentforthedetectionofphospholipidderivativesonthinlayerchromatograms
_version_	1721511585594212352

Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms.

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