Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms.
A simple modification of the Dittmer-Lester reagent is described that allow the detection of phospholipid derivatives at very low concentrations on silica gel and reversed-phase thin-layer plates. This modification, which involves the addition of acetic acid to the mixture, permits the observation o...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
1979-05-01
|
Series: | Journal of Lipid Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520406121 |
id |
doaj-82992b379d8b490c8b62aa46c582204a |
---|---|
record_format |
Article |
spelling |
doaj-82992b379d8b490c8b62aa46c582204a2021-04-24T05:52:53ZengElsevierJournal of Lipid Research0022-22751979-05-01204561563Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms.E K RyuM MacCossA simple modification of the Dittmer-Lester reagent is described that allow the detection of phospholipid derivatives at very low concentrations on silica gel and reversed-phase thin-layer plates. This modification, which involves the addition of acetic acid to the mixture, permits the observation of sharp blue spots on a white background. The specificity and sensitivity of the spray are discussed.http://www.sciencedirect.com/science/article/pii/S0022227520406121 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
E K Ryu M MacCoss |
spellingShingle |
E K Ryu M MacCoss Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms. Journal of Lipid Research |
author_facet |
E K Ryu M MacCoss |
author_sort |
E K Ryu |
title |
Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms. |
title_short |
Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms. |
title_full |
Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms. |
title_fullStr |
Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms. |
title_full_unstemmed |
Modification of the Dittmer-Lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms. |
title_sort |
modification of the dittmer-lester reagent for the detection of phospholipid derivatives on thin-layer chromatograms. |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
1979-05-01 |
description |
A simple modification of the Dittmer-Lester reagent is described that allow the detection of phospholipid derivatives at very low concentrations on silica gel and reversed-phase thin-layer plates. This modification, which involves the addition of acetic acid to the mixture, permits the observation of sharp blue spots on a white background. The specificity and sensitivity of the spray are discussed. |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520406121 |
work_keys_str_mv |
AT ekryu modificationofthedittmerlesterreagentforthedetectionofphospholipidderivativesonthinlayerchromatograms AT mmaccoss modificationofthedittmerlesterreagentforthedetectionofphospholipidderivativesonthinlayerchromatograms |
_version_ |
1721511585594212352 |