Effect of Adenosine Receptor Agonist and Ascorbic Acid on Ultrastructure of Hippocampal CA1 Neurons after Ischemia-Reperfusion Injury

• Main Authors: Mohammad Zamani, Mansoureh Soleimani, Akram Alizadeh, Majid Katebi
• Format: Article
• Language: fas
• Published: Vesnu Publications 2017-09-01
• Series: مجله دانشکده پزشکی اصفهان
• Subjects: Ischemia; CA1 region; Hippocampal; N6-cyclopentyladenosine; Ascorbic acid; Ultrastructure
• Online Access: http://jims.mui.ac.ir/index.php/jims/article/view/8250

Background: In brain ischemia, blood and oxygen supply decrease and after reperfusion, free radicals and reactive oxygen species (ROS) cause severe damage. As hippocampal injury after ischemia-reperfusion causes some complications, in this study we analyzed the effect of adenosine receptor agonist (N6-cyclopentyladenosine or CPA) and ascorbic acid on ultrastructure of hippocampal CA1 neurons after ischemia-reperfusion. Methods: 35 male rats in 5 groups were used. Ischemia-reperfusion performed by occlusion of common carotids for 15 minutes. CPA and ascorbic acids were intraperitoneally injected for 7 days after ischemia, and 2 weeks before and for 7 days after ischemia, respectively. After 20 days, brain samples were isolated, prepared, and assayed using transmission electron microscopy (TEM). Findings: Ultrastructure assay of hippocampal CA1 neurons after ischemia-reperfusion with transmission electron microscopy showed recovery of intracellular organelles particularly mitochondria of treated groups. In combination therapy, these improvements were better. Conclusion: Intraperitoneal injection of CPA and ascorbic acid after ischemia-reperfusion can reduce neural damage in CA1 region of hippocampus.