The profiling of microbiota in vaginal swab samples using 16S rRNA gene sequencing and IS-pro analysis
Abstract Background 16S rRNA gene sequencing is currently the most common way of determining the composition of microbiota. This technique has enabled many new discoveries to be made regarding the relevance of microbiota to the health and disease of the host. However, compared to other diagnostic te...
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doaj-82b880906eca4e949b5b338cd0763c522021-04-04T11:20:47ZengBMCBMC Microbiology1471-21802021-03-0121111010.1186/s12866-021-02149-7The profiling of microbiota in vaginal swab samples using 16S rRNA gene sequencing and IS-pro analysisM. Singer0R. Koedooder1M. P. Bos2L. Poort3S. Schoenmakers4P. H. M. Savelkoul5J. S. E. Laven6J. D. de Jonge7S. A. Morré8A. E. Budding9Laboratory of Immunogenetics, Department of Medical Microbiology and Infection Control, Amsterdam UMCDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Erasmus University Medical CenterInBiome B.VInBiome B.VDivision Obstetrics, Department of Obstetrics and Gynecology, Erasmus University Medical CentreDepartment of Medical Microbiology, Maastricht University Medical CenterDivision of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Erasmus University Medical CenterARTPred B.VLaboratory of Immunogenetics, Department of Medical Microbiology and Infection Control, Amsterdam UMCInBiome B.VAbstract Background 16S rRNA gene sequencing is currently the most common way of determining the composition of microbiota. This technique has enabled many new discoveries to be made regarding the relevance of microbiota to the health and disease of the host. However, compared to other diagnostic techniques, 16S rRNA gene sequencing is fairly costly and labor intensive, leaving room for other techniques to improve on these aspects. Results The current study aimed to compare the output of 16S rRNA gene sequencing to the output of the quick IS-pro analysis, using vaginal swab samples from 297 women of reproductive age. 16S rRNA gene sequencing and IS-pro analyses yielded very similar vaginal microbiome profiles, with a median Pearson’s R2 of 0.97, indicating a high level of similarity between both techniques. Conclusions We conclude that the results of 16S rRNA gene sequencing and IS-pro are highly comparable and that both can be used to accurately determine the vaginal microbiota composition, with the IS-pro analysis having the benefit of rapidity.https://doi.org/10.1186/s12866-021-02149-7Vaginal microbiotaIS-pro16S rRNA gene sequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M. Singer R. Koedooder M. P. Bos L. Poort S. Schoenmakers P. H. M. Savelkoul J. S. E. Laven J. D. de Jonge S. A. Morré A. E. Budding |
spellingShingle |
M. Singer R. Koedooder M. P. Bos L. Poort S. Schoenmakers P. H. M. Savelkoul J. S. E. Laven J. D. de Jonge S. A. Morré A. E. Budding The profiling of microbiota in vaginal swab samples using 16S rRNA gene sequencing and IS-pro analysis BMC Microbiology Vaginal microbiota IS-pro 16S rRNA gene sequencing |
author_facet |
M. Singer R. Koedooder M. P. Bos L. Poort S. Schoenmakers P. H. M. Savelkoul J. S. E. Laven J. D. de Jonge S. A. Morré A. E. Budding |
author_sort |
M. Singer |
title |
The profiling of microbiota in vaginal swab samples using 16S rRNA gene sequencing and IS-pro analysis |
title_short |
The profiling of microbiota in vaginal swab samples using 16S rRNA gene sequencing and IS-pro analysis |
title_full |
The profiling of microbiota in vaginal swab samples using 16S rRNA gene sequencing and IS-pro analysis |
title_fullStr |
The profiling of microbiota in vaginal swab samples using 16S rRNA gene sequencing and IS-pro analysis |
title_full_unstemmed |
The profiling of microbiota in vaginal swab samples using 16S rRNA gene sequencing and IS-pro analysis |
title_sort |
profiling of microbiota in vaginal swab samples using 16s rrna gene sequencing and is-pro analysis |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2021-03-01 |
description |
Abstract Background 16S rRNA gene sequencing is currently the most common way of determining the composition of microbiota. This technique has enabled many new discoveries to be made regarding the relevance of microbiota to the health and disease of the host. However, compared to other diagnostic techniques, 16S rRNA gene sequencing is fairly costly and labor intensive, leaving room for other techniques to improve on these aspects. Results The current study aimed to compare the output of 16S rRNA gene sequencing to the output of the quick IS-pro analysis, using vaginal swab samples from 297 women of reproductive age. 16S rRNA gene sequencing and IS-pro analyses yielded very similar vaginal microbiome profiles, with a median Pearson’s R2 of 0.97, indicating a high level of similarity between both techniques. Conclusions We conclude that the results of 16S rRNA gene sequencing and IS-pro are highly comparable and that both can be used to accurately determine the vaginal microbiota composition, with the IS-pro analysis having the benefit of rapidity. |
topic |
Vaginal microbiota IS-pro 16S rRNA gene sequencing |
url |
https://doi.org/10.1186/s12866-021-02149-7 |
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