Detection of Infectious Agents Causing Neonatal Calf Diarrhea on Two Large Dairy Farms in Yangxin County, Shandong Province, China
Neonatal calf diarrhea (NCD) is one of the most serious health challenges facing the livestock industry and has caused substantial economic losses due to increased morbidity and mortality rates. The present study investigated the main infectious pathogens causing NCD among cattle in Yangxin County,...
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Frontiers Media S.A.
2021-02-01
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Series: | Frontiers in Veterinary Science |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2020.589126/full |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaojuan Wei Xiaojuan Wei Xiaojuan Wei Weiwei Wang Weiwei Wang Weiwei Wang Zhen Dong Zhen Dong Zhen Dong Fusheng Cheng Fusheng Cheng Fusheng Cheng Xuzheng Zhou Xuzheng Zhou Xuzheng Zhou Bing Li Bing Li Bing Li Jiyu Zhang Jiyu Zhang Jiyu Zhang |
spellingShingle |
Xiaojuan Wei Xiaojuan Wei Xiaojuan Wei Weiwei Wang Weiwei Wang Weiwei Wang Zhen Dong Zhen Dong Zhen Dong Fusheng Cheng Fusheng Cheng Fusheng Cheng Xuzheng Zhou Xuzheng Zhou Xuzheng Zhou Bing Li Bing Li Bing Li Jiyu Zhang Jiyu Zhang Jiyu Zhang Detection of Infectious Agents Causing Neonatal Calf Diarrhea on Two Large Dairy Farms in Yangxin County, Shandong Province, China Frontiers in Veterinary Science neonatal calf diarrhea etiological agents China cattle Yangxin County |
author_facet |
Xiaojuan Wei Xiaojuan Wei Xiaojuan Wei Weiwei Wang Weiwei Wang Weiwei Wang Zhen Dong Zhen Dong Zhen Dong Fusheng Cheng Fusheng Cheng Fusheng Cheng Xuzheng Zhou Xuzheng Zhou Xuzheng Zhou Bing Li Bing Li Bing Li Jiyu Zhang Jiyu Zhang Jiyu Zhang |
author_sort |
Xiaojuan Wei |
title |
Detection of Infectious Agents Causing Neonatal Calf Diarrhea on Two Large Dairy Farms in Yangxin County, Shandong Province, China |
title_short |
Detection of Infectious Agents Causing Neonatal Calf Diarrhea on Two Large Dairy Farms in Yangxin County, Shandong Province, China |
title_full |
Detection of Infectious Agents Causing Neonatal Calf Diarrhea on Two Large Dairy Farms in Yangxin County, Shandong Province, China |
title_fullStr |
Detection of Infectious Agents Causing Neonatal Calf Diarrhea on Two Large Dairy Farms in Yangxin County, Shandong Province, China |
title_full_unstemmed |
Detection of Infectious Agents Causing Neonatal Calf Diarrhea on Two Large Dairy Farms in Yangxin County, Shandong Province, China |
title_sort |
detection of infectious agents causing neonatal calf diarrhea on two large dairy farms in yangxin county, shandong province, china |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Veterinary Science |
issn |
2297-1769 |
publishDate |
2021-02-01 |
description |
Neonatal calf diarrhea (NCD) is one of the most serious health challenges facing the livestock industry and has caused substantial economic losses due to increased morbidity and mortality rates. The present study investigated the main infectious pathogens causing NCD among cattle in Yangxin County, China. Sixty-nine fecal samples were collected from diarrheic newborn cattle and tested for infectious agents, including bovine rotavirus, bovine coronavirus, Escherichia coli K99, Cryptosporidium parvum, and Giardia lamblia, that cause NCD, as determined by rapid kit analysis and polymerase chain reaction (PCR) amplification. The PCR results showed that the percentages of samples that were positive for C. parvum, bovine rotavirus A, bovine coronavirus, and G. lamblia were 44.93, 36.23, 17.39, and 13.04%, respectively. The rapid kit analysis results showed that the prevalence of C. parvum, rotavirus, coronavirus, and G. lamblia was 52.17, 31.88, 28.98, and 18.84%, respectively. No E. coli K99 was detected by either method. The total positivity of the samples, as determined by PCR and rapid kit analysis, was 80.00 and 81.16%, respectively. No significant difference between the two methods was observed. The results of this study may help to establish a foundation for future research investigating the epidemiology of NCD in cattle and may facilitate the implementation of measures to control NCD transmission to cattle in Yangxin County, Shandong Province, China. |
topic |
neonatal calf diarrhea etiological agents China cattle Yangxin County |
url |
https://www.frontiersin.org/articles/10.3389/fvets.2020.589126/full |
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doaj-82bd2e75050f43fbaca5d29f2a9ed1e22021-02-05T04:44:55ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-02-01710.3389/fvets.2020.589126589126Detection of Infectious Agents Causing Neonatal Calf Diarrhea on Two Large Dairy Farms in Yangxin County, Shandong Province, ChinaXiaojuan Wei0Xiaojuan Wei1Xiaojuan Wei2Weiwei Wang3Weiwei Wang4Weiwei Wang5Zhen Dong6Zhen Dong7Zhen Dong8Fusheng Cheng9Fusheng Cheng10Fusheng Cheng11Xuzheng Zhou12Xuzheng Zhou13Xuzheng Zhou14Bing Li15Bing Li16Bing Li17Jiyu Zhang18Jiyu Zhang19Jiyu Zhang20Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences (CAAS), Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Lanzhou, ChinaKey Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences (CAAS), Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Lanzhou, ChinaKey Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences (CAAS), Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Lanzhou, ChinaKey Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences (CAAS), Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Lanzhou, ChinaKey Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences (CAAS), Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Lanzhou, ChinaKey Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences (CAAS), Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Lanzhou, ChinaKey Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, ChinaLanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences (CAAS), Lanzhou, ChinaKey Laboratory of New Animal Drug Project of Gansu Province, Lanzhou, ChinaKey Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, ChinaNeonatal calf diarrhea (NCD) is one of the most serious health challenges facing the livestock industry and has caused substantial economic losses due to increased morbidity and mortality rates. The present study investigated the main infectious pathogens causing NCD among cattle in Yangxin County, China. Sixty-nine fecal samples were collected from diarrheic newborn cattle and tested for infectious agents, including bovine rotavirus, bovine coronavirus, Escherichia coli K99, Cryptosporidium parvum, and Giardia lamblia, that cause NCD, as determined by rapid kit analysis and polymerase chain reaction (PCR) amplification. The PCR results showed that the percentages of samples that were positive for C. parvum, bovine rotavirus A, bovine coronavirus, and G. lamblia were 44.93, 36.23, 17.39, and 13.04%, respectively. The rapid kit analysis results showed that the prevalence of C. parvum, rotavirus, coronavirus, and G. lamblia was 52.17, 31.88, 28.98, and 18.84%, respectively. No E. coli K99 was detected by either method. The total positivity of the samples, as determined by PCR and rapid kit analysis, was 80.00 and 81.16%, respectively. No significant difference between the two methods was observed. The results of this study may help to establish a foundation for future research investigating the epidemiology of NCD in cattle and may facilitate the implementation of measures to control NCD transmission to cattle in Yangxin County, Shandong Province, China.https://www.frontiersin.org/articles/10.3389/fvets.2020.589126/fullneonatal calf diarrheaetiological agentsChinacattleYangxin County |