In vivo MRS measurement of liver lipid levels in mice
A magnetic resonance spectroscopy (MRS) procedure for in vivo measurement of lipid levels in mouse liver is described and validated. The method uses respiratory-gated, localized spectroscopy to collect proton spectra from voxels within the mouse liver. Bayesian probability theory analysis of these s...
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doaj-82cb9c21566e421d922d948137f083942021-04-27T04:40:45ZengElsevierJournal of Lipid Research0022-22752004-07-0145713641371In vivo MRS measurement of liver lipid levels in miceJ.R. Garbow0X. Lin1N. Sakata2Z. Chen3D. Koh4G. Schonfeld5Department of Chemistry, Washington University, St. Louis, MO 63130; Departments of Radiology, Washington University School of Medicine, St. Louis, MO 63110; Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110; The Alvin J. Siteman Cancer Center, Washington University School of Medicine, St. Louis, MO 63110Department of Chemistry, Washington University, St. Louis, MO 63130; Departments of Radiology, Washington University School of Medicine, St. Louis, MO 63110; Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110; The Alvin J. Siteman Cancer Center, Washington University School of Medicine, St. Louis, MO 63110Department of Chemistry, Washington University, St. Louis, MO 63130; Departments of Radiology, Washington University School of Medicine, St. Louis, MO 63110; Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110; The Alvin J. Siteman Cancer Center, Washington University School of Medicine, St. Louis, MO 63110Department of Chemistry, Washington University, St. Louis, MO 63130; Departments of Radiology, Washington University School of Medicine, St. Louis, MO 63110; Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110; The Alvin J. Siteman Cancer Center, Washington University School of Medicine, St. Louis, MO 63110Department of Chemistry, Washington University, St. Louis, MO 63130; Departments of Radiology, Washington University School of Medicine, St. Louis, MO 63110; Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110; The Alvin J. Siteman Cancer Center, Washington University School of Medicine, St. Louis, MO 63110Department of Chemistry, Washington University, St. Louis, MO 63130; Departments of Radiology, Washington University School of Medicine, St. Louis, MO 63110; Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110; The Alvin J. Siteman Cancer Center, Washington University School of Medicine, St. Louis, MO 63110A magnetic resonance spectroscopy (MRS) procedure for in vivo measurement of lipid levels in mouse liver is described and validated. The method uses respiratory-gated, localized spectroscopy to collect proton spectra from voxels within the mouse liver. Bayesian probability theory analysis of these spectra allows the relative intensities of the lipid and water resonances within the liver to be accurately measured. All spectral data were corrected for measured spin-spin relaxation. A total of 48 mice were used in this study, including wild-type mice and two different transgenic mouse strains. Different groups of these mice were fed high-fat or low-fat diets or liquid diets with and without the addition of alcohol. Proton spectra were collected at baseline and, subsequently, every 4 weeks for up to 16 weeks. Immediately after the last MRS measurement, mice were killed and their livers analyzed for triglyceride level by conventional wet-chemistry methods. The excellent correlation between in vivo MRS and ex vivo wet-chemistry determinations of liver lipids validates the MRS method.These results clearly demonstrate that in vivo MRS will be an extremely valuable technique for longitudinal studies aimed at providing important insights into the genetic, environmental, and dietary factors affecting fat deposition and accumulation within the mouse liver.http://www.sciencedirect.com/science/article/pii/S0022227520317934magnetic resonance spectroscopytriglyceridefatty livercirrhosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
J.R. Garbow X. Lin N. Sakata Z. Chen D. Koh G. Schonfeld |
spellingShingle |
J.R. Garbow X. Lin N. Sakata Z. Chen D. Koh G. Schonfeld In vivo MRS measurement of liver lipid levels in mice Journal of Lipid Research magnetic resonance spectroscopy triglyceride fatty liver cirrhosis |
author_facet |
J.R. Garbow X. Lin N. Sakata Z. Chen D. Koh G. Schonfeld |
author_sort |
J.R. Garbow |
title |
In vivo MRS measurement of liver lipid levels in mice |
title_short |
In vivo MRS measurement of liver lipid levels in mice |
title_full |
In vivo MRS measurement of liver lipid levels in mice |
title_fullStr |
In vivo MRS measurement of liver lipid levels in mice |
title_full_unstemmed |
In vivo MRS measurement of liver lipid levels in mice |
title_sort |
in vivo mrs measurement of liver lipid levels in mice |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2004-07-01 |
description |
A magnetic resonance spectroscopy (MRS) procedure for in vivo measurement of lipid levels in mouse liver is described and validated. The method uses respiratory-gated, localized spectroscopy to collect proton spectra from voxels within the mouse liver. Bayesian probability theory analysis of these spectra allows the relative intensities of the lipid and water resonances within the liver to be accurately measured. All spectral data were corrected for measured spin-spin relaxation. A total of 48 mice were used in this study, including wild-type mice and two different transgenic mouse strains. Different groups of these mice were fed high-fat or low-fat diets or liquid diets with and without the addition of alcohol. Proton spectra were collected at baseline and, subsequently, every 4 weeks for up to 16 weeks. Immediately after the last MRS measurement, mice were killed and their livers analyzed for triglyceride level by conventional wet-chemistry methods. The excellent correlation between in vivo MRS and ex vivo wet-chemistry determinations of liver lipids validates the MRS method.These results clearly demonstrate that in vivo MRS will be an extremely valuable technique for longitudinal studies aimed at providing important insights into the genetic, environmental, and dietary factors affecting fat deposition and accumulation within the mouse liver. |
topic |
magnetic resonance spectroscopy triglyceride fatty liver cirrhosis |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520317934 |
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